Biogeochemical implications of comparative growth rates of <i>Emiliania huxleyi</i> and <i>Coccolithus</i> species

Abstract

Abstract. Coccolithophores, a diverse group of phytoplankton, make important contributions to pelagic calcite production and export, yet the comparative biogeochemical role of species other than the ubiquitous Emiliania huxleyi is poorly understood. The contribution of different coccolithophore species to total calcite production is controlled by interspecies differences in cellular calcite, growth rate and relative abundance within a mixed community. In this study we examined the relative importance of E. huxleyi and two Coccolithus species in terms of daily calcite production. Culture experiments compared growth rates and cellular calcite content of E. huxleyi (Arctic and temperate strains), Coccolithus pelagicus (novel Arctic strain) and Coccolithus braarudii (temperate strain). Despite assumptions that E. huxleyi is a fast-growing species, growth rates between the three species were broadly comparable (0.16-0.85 d −1 ) under identical temperature and light conditions. Emiliania huxleyi grew only 12 % faster on average than C. pelagicus, and 28 % faster than C. braarudii. As the cellular calcite content of C. pelagicus and C. braarudii is typically 30-80 times greater than E. huxleyi, comparable growth rates suggest that Coccolithus species have the potential to be major calcite producers in mixed populations. To further explore these results we devised a simplistic model comparing daily calcite production from Coccolithus and E. huxleyi across a realistic range of relative abundances and a wide range of relative growth rates. Using the relative differences in growth rates from our culture studies, we found that C. pelagicus would be a larger source of calcite if abundances of E. huxleyi to C. pelagicus were below 34 : 1. Relative abundance data collected from North Atlantic field samples (spring and summer 2010) suggest that, with a relative growth rate of 88 %, C. pelagicus dominated calcite production at 69 % of the sites sampled. With a more extreme difference in growth rates, where C. pelagicus grows at 1 / 10th of the rate of E. huxleyi, C. pelagicus still dominated calcite production in 14 % of the field. These results demonstrate the necessity of considering interactions between inter-species differences in growth rates, cellular calcite and relative abundances when evaluating the contribution of different coccolithophores to pelagic calcite production. In the case of C. pelagicus, we find that there is strong potential for this species to make major contributions to calcite production in the North Atlantic, although estimates of relative growth rates from the field are needed to confirm our conclusions.

Physiology regulates the relationship between coccosphere geometry and growth phase in coccolithophores

“…We also present results from a previously unanalysed data set of exponential-phase coccosphere geometry in C. braarudii strain RCC 1198 and C. pelagicus strain RCC 4092, originally published as a data report by and available from http://www.pangaea.de (doi:10.1594/PANGAEA.836841). For that study, batch culture experiments were undertaken at multiple temperatures (6-12 • C in C. pelagicus and 12-19 • C in C. braarudii), and samples for coccosphere geometry analysis were collected on a single mid-exponential-phase experiment day (further details in Daniels et al, 2014). …”

“…Calcite production per cell per day (pmol C cell −1 d −1 ) can be calculated by multiplying cellular calcite (pmol C cell −1 ) by growth rate (d −1 ) (e.g. Daniels et al, 2014Daniels et al, , 2016. Calcite production in these four species is 6 to 20 times higher than in E. huxleyi at a comparable growth rate ( Fig.…”

“…For comparison purposes, we include data for C. braarudii and C. pelagicus that can be found in Gibbs et al (2013) and accompanying Daniels et al (2014).…”

“…All LM analysis was performed using a cross-polarised light microscope (Olympus BX51) with a colour camera attached (Olympus DP71). Coccosphere geometry data were obtained through LM following the same techniques applied by Gibbs et al (2013) and Daniels et al (2014) and described in detail here. Random transects across the widest section of the filter hemisphere were performed until 30 individual coccospheres per slide were located from slides corresponding to alternate day or, Figure 1.…”

Characterization of a Time-Domain Dual Lifetime Referencing pCO2 Optode and Deployment as a High-Resolution Underway Sensor across the High Latitude North Atlantic Ocean

“…The phytoplankton Emiliania huxleyi (coccolithophore, Strain RCC1228 from the Roscoff Collection) was cultured in artificial seawater at 16 • C, under light conditions of 100 µE. The chlorophyll concentration in the stock solution was calculated based on a chlorophyll concentration per cell (Daniels et al, 2014), and the counted cell concentration of the stock solution. The culture was diluted with artificial seawater to 100 ml, with final chlorophyll concentrations of 0.1, 1, 2, 2.5, 5, 10 µg L −1 .…”