Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa

Abstract

Bacterial infections are the key cause of death in patients suffering from burns and diabetic wounds while the use of traditional antibiotics has been growing steadily. Thus, in the present study, we are trying to introduce a paradigm shift strategy to improve chronic wound healing of bacterial infection. To that end, we have biologically synthesized silver nanoparticles (AgNPs) using Arthrospira sp polysaccharides, and evaluated their antibacterial efficacy with their safety pattern. Scanning electron micrographs showed spherical AgNPs coated with algal polysaccharides with an approximate size of 9.7 nm. Treatment of Pseudomonas aeruginosa with the AgNPs (0.5–1 μg/mL) resulted in a significant disruption in P. aeruginosa outer membrane, reduction in biofilm formation, and a significant decrease of production of alginate and pyocyanin along with a concentration-dependent reduction in β-lactamase activity. In addition, at the in vivo level, AgNPs displayed substantial activity to control P. aeruginosa infections in rat skin wounds with significant reduction in in COX-2 enzyme in both rat skin homogenate and serum samples. Furthermore, AgNPs facilitated wound curative in the P. aeruginosa infected model by reducing the hemorrhagic areas number and the infiltrated inflammatory cells. Taken all together, these biogenic nanoparticles showed unique properties in controlling bacterial wound infections and improving the healing process of damaged tissues via its direct and indirect effects.

NFκB-Activated COX2/PGE2/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues

“…And finally, the inflammatory mediators’ primers are IDO1 (#Hs00984148_m1), IL-10 (#Hs00961622_m1), NOS2 (#Hs01075529_m1) and TNFα (#Hs00174128_m1). HPRT1 primer (#Hs02800695_m1) was used as a housekeeping gene [ 7 , 34 , 72 ]. All TaqMan primers were obtained from Thermo Fisher Scientific Life Technologies, Cambridge, MA, USA.…”

Nanomaterials in Skin Regeneration and Rejuvenation

“…Moreover, the new nanomaterial was less cytotoxic to fibroblasts than SSD alone and showed faster wound healing compared to other SSD and SSD-SLNs preparations. Of course, other nanomaterials showed to be effective against biofilms in wounds, but they are all manly based on silver nanoparticles, either alone or in combination [130][131][132][133].…”

Biotechnological Applications of Polymeric Nanofiber Platforms Loaded with Diverse Bioactive Materials

“…Many studies have investigated electrospun NFs with antimicrobial activity produced using biopolymers such as chitosan and its derivatives, or by blending chitosan with other polymers such as polyvinyl alcohol (PVA) [99] and poly (ethylene oxide) (PEO) [100,101]. In order to confer antimicrobial properties to NFs, different antimicrobial agents can be incorporated into the nanofibrous structure [125][126][127][128][129][130][131][132][133][134][135][136]. Antimicrobial agents such as antibiotics (e.g., tetracycline, ciprofloxacin, gentamicin etc.…”