Biogenic ZnO Nanoparticles Synthesized from Origanum vulgare Abrogates Quorum Sensing and Biofilm Formation in Opportunistic Pathogen Chromobacterium violaceum

Kamli, Majid Rasool; Malik, Maqsood Ahmad; Srivastava, Vartika; Sabir, Jamal S. M.; Mattar, Ehab H.; Ahmad, Aijaz

doi:10.3390/pharmaceutics13111743

Cited by 14 publications

(9 citation statements)

References 111 publications

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“…In conclusion, treatment with Ag-Cu NPs inhibited biofilm development by 10–40%, with the most considerable inhibition observed at a dose of 50 µg/mL ( Figure 7 ). Our results are consistent with our previous studies in which ZnO NPs altered biofilm formation by C. violaceum [ 62 ]. Biofilm inhibition by metallic nanoparticles has been reported in many studies.…”

Section: Resultssupporting

confidence: 94%

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Biosynthesis of Novel Ag-Cu Bimetallic Nanoparticles from Leaf Extract of Salvia officinalis and Their Antibacterial Activity

Malik

Albeladi

Al-Maaqar

et al. 2023

Life

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Bimetallic nanoparticles exhibit bifunctional or synergistic effects prevailing between two metals with the capabilities of enhanced electronic, catalytic, and optical properties. Green synthetic routes have gained tremendous interest because of the noninvolvement of toxic and harmful chemical reagents in preparation. Therefore, we develop bimetallic Ag-Cu nanoparticles (Ag-Cu NPs) through an eco-friendly and biocompatible preparation method. In this study, Ag-Cu NPs have been synthesized from leaf extracts of the commonly known sage, S. officinalis. The extract has a rich phytochemical composition, including bioreducing polyphenols, flavonoids, and capping/stabilizing agents. An array of well-known spectroscopic and microscopic techniques were used to characterize the as-prepared Ag-Cu bimetallic nanoparticles, including X-ray diffraction (XRD), ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The size of the Ag-Cu NPs was found to be 50 nm with a spherical shape and an almost uniform distribution. The antibacterial effect was further evaluated using agar well diffusion and disc diffusion assays. Ag-Cu NPs exhibit antibacterial and antibiofilm properties against Gram-positive and Gram-negative bacteria strains. The minimum inhibitory concentration (MIC) of Ag-Cu NPs was between 5 g/mL and 15 g/mL. The Ag-Cu NPs inhibit biofilm formation at 25 g/mL and 50 g/mL. The results of biogenic Ag-Cu NPs provide novel antibacterial activity against Gram-positive and Gram-negative bacteria, as well as antibiofilm activity. Hence, Ag-Cu NPs might serve as a novel antibacterial agent with potential antibacterial and antibiofilm properties.

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Section: Resultssupporting

confidence: 94%

“…The MIC results showed that Ag-Cu NPs have potent antibacterial activity. The results follow previous studies in which synthesized ZnO NPs showed activity against an opportunistic pathogen, Chromobacterium violaceum [ 62 ].…”

Section: Resultssupporting

confidence: 90%

Biosynthesis of Novel Ag-Cu Bimetallic Nanoparticles from Leaf Extract of Salvia officinalis and Their Antibacterial Activity

Malik

Albeladi

Al-Maaqar

et al. 2023

Life

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“…XRD analysis revealed the average size (nm) for the three samples from hydroethanolic extrats of Artemisia annua (A-@1 mgL -1 , B-@3 mgL -1 , C-@5 mgL -1 ) as 21.34, 24.00 and 24.71, respectively. The particle average sizes obtained for the synthesized ZnONPs were in agreement with the previous findings [50].…”

Section: Xrd Analysissupporting

confidence: 91%

“…4.A) as reported for Eichhornia crassipes leaf extract [51], H. rosa-sinensis [52], Calotropis gigantean [53] and Zingiber officinale [54]. The carbon peak observed could result from some carbon deposition during the calcination of the ZnONPs [50]. The purity, elemental composition and stoichiometry of the synthesized ZnONPs were performed by using EDS analysis.…”

Section: Sem-eds Analysismentioning

confidence: 77%

Green synthesis and characterization of zinc oxide nanoparticles using extracts of Artemisia annua l. grown in Togo

Sesime¹,

Dzagli²,

Afoudji³

2021

J. Optoelectron. Biomed. M.

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Plant-mediated synthesis of ZnO nanoparticles (ZnONPs) is preferable than the conventional methods with many applications in medicine and biology. Artemisia annua is recognized to have antiplasmodial, antimicrobial activities, so the nanoformulation based on its extracts and zinc oxide would be of therapeutic benefit. This study aims to investigate properties of ZnONPs based on Artemisia annua leaves and stems extracts and zinc acetate. Spectroscopy techniques, XRD, SEM and Energy Dispersive X-ray Spectroscopy (EDS) were used to investigate the structural and optical properties of the ZnONPs. The characteristic absorption peak was at 375.5 nm and the excitation at 365 nm showed wide spectra in visible (450-700 nm). XRD, SEM and EDS analysis confirmed a pure ZnONPs in wurtzite hexagonal structure with a size of 21.34 - 24.71 nm. These nanoformulations would be multifunctional and are candidate for treatment of malaria, cosmetics and optoelectronic.

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“…The biogenic ZnO NPs possess the ability to penetrate through the biofilm and act on the cells by bringing degradation in the genomic DNA and RNA content of the cell (Kamli et al, 2021 ). The biogenic ZnO NPs brought a marked reduction in the genomic DNA content of A. faecalis and P. gingivalis by 94.26 ± 1.09% and 96.23 ± 1.89%, respectively.…”

Section: Characterization Of the Green Synthesized Zno Npsmentioning

confidence: 99%

Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study

et al. 2022

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The development of biofilm on the biotic and abiotic surfaces is the greatest challenge for health care sectors. At present times, oral infection is a common concern among people with an unhealthy lifestyle and most of these biofilms-associated infections are resistant to antibiotics. This has increased a search for the development of alternate therapeutics for eradicating biofilm-associated infection. Nanobiotechnology being an effective way to combat such oral infections may encourage the use of herbal compounds, such as bio-reducing and capping agents. Green-synthesis of ZnO nanoparticles (ZnO NP) by the use of the floral extract of Clitoria ternatea, a traditionally used medicinal plant, showed stability for a longer period of time. The NPs as depicted by the TEM image with a size of 10 nm showed excitation spectra at 360 nm and were found to remain stable for a considerable period of time. It was observed that the NPs were effective in the eradication of the oral biofilm formed by the major tooth attacking bacterial strains namely Porphyromonsas gingivalis and Alcaligenes faecalis, by bringing a considerable reduction in the extracellular polymeric substances (EPS). It was observed that the viability of the Porphyromonsas gingivalis and Alcaligenes faecalis was reduced by NP treatment to 87.89 ± 0.25% in comparison to that of amoxicillin. The results went in agreement with the findings of modeling performed by the use of response surface methodology (RSM) and artificial neural network (ANN). The microscopic studies and FT-IR analysis revealed that there was a considerable reduction in the biofilm after NP treatment. The in silico studies further confirmed that the ZnO NPs showed considerable interactions with the biofilm-forming proteins. Hence, this study showed that ZnO NPs derived from Clitoria ternatea can be used as an effective alternative therapeutic for the treatment of biofilm associated oral infection.

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Biogenic ZnO Nanoparticles Synthesized from Origanum vulgare Abrogates Quorum Sensing and Biofilm Formation in Opportunistic Pathogen Chromobacterium violaceum

Cited by 14 publications

References 111 publications

Biosynthesis of Novel Ag-Cu Bimetallic Nanoparticles from Leaf Extract of Salvia officinalis and Their Antibacterial Activity

Biosynthesis of Novel Ag-Cu Bimetallic Nanoparticles from Leaf Extract of Salvia officinalis and Their Antibacterial Activity

Green synthesis and characterization of zinc oxide nanoparticles using extracts of Artemisia annua l. grown in Togo

Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study

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