“…The microbial colonization on glass windows is related to the climate and to a high concentration of air pollutants, among other factors. The main effects of biogenic attack on historical glass, include etching, pitting, leaching, discoloration, glass degradation, formation of gel layers, and intense soiling on the original glass surface, as reported in the literature (Drewello et al, 2000;Lombardo et al, 2005;Piñar et al, 2013;Rodrigues et al, 2014;Shirakawa et al, 2016). Most of these effects induce the loss of transparency, which directly affects not only the stained glass aesthetics, but also its iconographic function (Carmona et al, 2006).…”