Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro

Mata, Rani; Nakkala, Jayachandra Reddy; Rani, Sapna

doi:10.1016/j.colsurfb.2015.01.052

Cited by 135 publications

(53 citation statements)

References 48 publications

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“…These findings confirm the cytotoxic potential of FSNPs which mediates its activity through induction of apoptosis. Our AO/EB results were also supported by the earlier report on biogenic silver nanoparticles from Abutilon indicum leaf extract which emphasize the apoptosis mediated cytotoxicity in COLO 205 cells [46].…”

Section: Resultssupporting

confidence: 92%

Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract

Mata

Nakkala

Rani

2015

Materials Science and Engineering: C

Self Cite

103

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Section: Resultssupporting

confidence: 92%

Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract

Mata

Nakkala

Rani

2015

Materials Science and Engineering: C

Self Cite

103

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“…ions may contribute to the production of superoxide and hydrogen peroxide in the membranes of mitochondria (Yang et al 2012). Our results are in good accordance with an earlier report on the H 2 O 2 scavenging effect of leaf extract of Abutilon indicum (Mata et al 2015).…”

Section: Measurement Of H 2 O 2 Scavenging Assaysupporting

confidence: 82%

Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity

Bhakya¹,

Muthukrishnan

Sukumaran³

et al. 2015

Appl Nanosci

368

178

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Nanomedicine utilizes biocompatible nanomaterials for diagnostic and therapeutic purposes. The present study reports the use of Helicteres isora root extract for the synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were initially noticed through visual color change from yellow to reddish brown and further confirmed by surface plasmonic resonance (SPR) band at 450 nm using UV-visible spectroscopy. Morphology and size of AgNPs were determined by transmission electron microscopy (TEM) analysis. X-ray diffraction (XRD) study revealed crystalline nature of AgNPs. The prolonged stability of AgNPs was due to capping of oxidized polyphenols and carboxyl protein which was established by Fourier transform infrared spectroscopy (FTIR) study. In addition, the synthesized AgNPs were tested for antioxidant and antibacterial activities. It showed good antioxidant activity as compared to butylated hydroxytoluene (BHT) and ascorbic acid as standard antioxidant. It could be concluded that H. isora root extract can be used efficiently in the production of potential antioxidant and antibacterial AgNPs for commercial application.

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“…Plant-assisted AgNPs showed greater anti-proliferative effects against HCT116 cells at a minimum concentration of approximately 450 nM than synthetic AgNPs and AgNO 3 [36]. Abutilon indicum -assisted AgNPs showed dose-dependent anti-proliferative effects against COLO 205 (human colon cancer) and MDCK (normal) cells [37]. …”

Section: Resultsmentioning

confidence: 99%

“…Miethling-Graff et al [34] reported the size-dependent toxicity by generation of ROS in the human LoVo cell line, in which smaller-sized particles produced more ROS than larger-sized particles. Mata et al [37] found that COLO205 cells exposed to AgNPs produced significant levels of ROS compared to untreated control cells. The major anti-cancer effect of AgNPs involves the induction of apoptosis and autophagy through various molecular mechanisms, including by significantly decreasing cell viability and motility, impairing matrix metalloproteinase-2 and -9 activity, and promoting ROS generation, all of which induce cell death through apoptosis and autophagy [47].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

Gurunathan

Qasim

Park

et al. 2018

IJMS

137

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Silver nanoparticles (AgNPs) have gained attention for use in cancer therapy. In this study, AgNPs were biosynthesized using naringenin. We investigated the anti-colon cancer activities of biogenic AgNPs through transcriptome analysis using RNA sequencing, and the mechanisms of AgNPs in regulating colon cancer cell growth. The synthesized AgNPs were characterized using UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The AgNPs were spherical with sizes of 2–10 nm. Cytotoxicity assays indicated that the AgNPs in HCT116 colorectal cancer cells were very effective at low concentrations. The viability and proliferation of colon cancer cells treated with 5 µg/mL biogenic AgNPs were reduced by 50%. Increased lactate dehydrogenase leakage (LDH), reactive oxygen species (ROS) generation, malondialdehyde (MDA), and decreased dead-cell protease activity and ATP generation were observed. This impaired mitochondrial function and DNA damage led to cell death. The AgNPs upregulated and downregulated the most highly ranked biological processes of oxidation–reduction and cell-cycle regulation, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that AgNPs upregulated GADD45G in the p53 pathway. Thus, the AgNP tumor suppressive effects were mediated by cell apoptosis following DNA damage, as well as by mitochondrial dysfunction and cell-cycle arrest following aberrant regulation of p53 effector proteins. It is of interest to mention that, to the best of our knowledge, this study is the first report demonstrating cellular responses and molecular pathways analysis of AgNPs in HCT116 colorectal cancer cells.

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Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro

Cited by 135 publications

References 48 publications

Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract

Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract

Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity

Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

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