Biogenic gas emissions from soils measured using a new automated laboratory incubation system

Cardenas, L. M.; Hawkins, J. M. B.; Chadwick, David R.; Scholefield, D.

doi:10.1016/s0038-0717(03)00092-0

Cited by 79 publications

(87 citation statements)

References 13 publications

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“…The groups (treatments 1-3) of cores were used for three separate N-loss incubation assays (Jahangir et al 2012). Briefly, the base of each core was leveled out avoiding smearing and all vegetation was removed, so that the corer and soil fit exactly inside cylindrical incubation vessels in a specialized incubation chamber (Cardenas et al 2003;Jahangir et al 2012). The vessels were sealed to exclude atmospheric N 2 , preincubated at 15°C and purged from the bottom with a He/O 2 mixture (80:20) for 24 h (known as the flow-through method with a flow rate of 20 ml min −1 ) to replace most of the original atmosphere, and then for another 72 h across the headspace in flow-over mode at 10 ml min −1 to remove the remaining N 2 (Cardenas et al 2003;Jahangir et al 2012).…”

Section: N-loss Assaysmentioning

confidence: 99%

“…For real-time N-loss analysis, temporal N 2 and N 2 O evolution was measured using dedicated gas chromatographs as described by Jahangir et al (2012) in an automated incubation system ( Fig. 1; Cardenas et al 2003;Scholefield et al 1997a;Scholefield et al 1997b) at Rothamsted Research, North Wyke, Devon, UK. Sampling times for microbial analysis were chosen to reflect nitrogenous emissions; therefore, sampling times changed according to the behavior in the emission signal.…”

Section: N-loss Assaysmentioning

confidence: 99%

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Carbon amendment and soil depth affect the distribution and abundance of denitrifiers in agricultural soils

Barrett

Khalil

Jahangir

et al. 2016

Environ Sci Pollut Res

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The nitrite reductase (nirS and nirK) and nitrous oxide reductase-encoding (nosZ) genes of denitrifying populations present in an agricultural grassland soil were quantified using real-time polymerase chain reaction (PCR) assays. Samples from three separate pedological depths at the chosen site were investigated: horizon A (0-10 cm), horizon B (45-55 cm), and horizon C (120-130 cm). The effect of carbon addition (treatment 1, control; treatment 2, glucose-C; treatment 3, dissolved organic carbon (DOC)) on denitrifier gene abundance and N2O and N2 fluxes was determined. In general, denitrifier abundance correlated well with flux measurements; nirS was positively correlated with N2O, and nosZ was positively correlated with N2 (P < 0.03). Denitrifier gene copy concentrations per gram of soil (GCC) varied in response to carbon type amendment (P < 0.01). Denitrifier GCCs were high (ca. 10(7)) and the bac:nirK, bac:nirS, bac:nir (T) , and bac:nosZ ratios were low (ca. 10(-1)/10) in horizon A in all three respective treatments. Glucose-C amendment favored partial denitrification, resulting in higher nir abundance and higher N2O fluxes compared to the control. DOC amendment, by contrast, resulted in relatively higher nosZ abundance and N2 emissions, thus favoring complete denitrification. We also noted soil depth directly affected bacterial, archaeal, and denitrifier abundance, possibly due to changes in soil carbon availability with depth.

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Section: N-loss Assaysmentioning

confidence: 99%

Section: N-loss Assaysmentioning

confidence: 99%

Carbon amendment and soil depth affect the distribution and abundance of denitrifiers in agricultural soils

Barrett

Khalil

Jahangir

et al. 2016

Environ Sci Pollut Res

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“…In agriculture, one of the major pathways of manure N loss to the environment are gaseous emission of ammonia (NH 3 ) (Webb et al, 2005), N 2 O (Chadwick et al, 2011), N 2 (Cardenas et al, 2003), and the leaching of nitrate through soil . After NH 3 is volatilized, it combines with other chemicals in the atmosphere to form particulates that can adversely affect human health (Erisman et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Improving manure nutrient management towards sustainable agricultural intensification in China

Chadwick

Jia

Tong

et al. 2015

Agriculture, Ecosystems & Environment

283

154

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“…Hauck and Melsted, 1956;Rolston et al, 1978Rolston et al, , 1982Mosier et al, 1986), (3) direct N 2 quantification by completely replacing N 2 in the soil samples with, for example, He (e.g. Scholefield et al, 1997;Butterbach-Bahl et al, 2002;Cardenas et al, 2003), and (4) mass balance approaches (e.g. Allison, 1955).…”

Section: Introductionmentioning

confidence: 99%

Theoretical and practical limitations of the acetylene inhibition technique to determine total denitrification losses

et al. 2012

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Abstract. The loss of N 2 from intensively managed agroecosystems is an important part of the N budget. Flux monitoring of N 2 emissions at the field scale, e.g., by eddy correlation or aerodynamic gradient method, is impossible due to the large atmospheric N 2 background (78 %). The acetylene (C 2 H 2 ) inhibition technique (AIT) is a rather simple and frequently used, albeit imperfect, method to determine N 2 losses from intact soil cores. In principle, AIT allows an estimation of total denitrification at high temporal resolution and on small spatial scales, with limited workload and costs involved. To investigate its potential and limitations, a laboratory system with two different detection systems (photoacoustic IR spectroscopy and gas chromatography) is presented, which allowed simultaneous measurements of up to 7 intact soil cores in air-tight glass tubes in a temperature controlled cabinet (adjusted to field conditions) with automated C 2 H 2 injection.A survey of total denitrification losses (N 2 + N 2 O) over 1.5 yr in soil cores from an intensively managed, cut grassland system in central Switzerland supports previous reports on severe limitations of the AIT, which precluded reliable estimates of total denitrification losses. Further, the unavoidable sampling and transfer of soil samples to the laboratory causes unpredictable deviations from the denitrification activity in the field.

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Biogenic gas emissions from soils measured using a new automated laboratory incubation system

Cited by 79 publications

References 13 publications

Carbon amendment and soil depth affect the distribution and abundance of denitrifiers in agricultural soils

Carbon amendment and soil depth affect the distribution and abundance of denitrifiers in agricultural soils

Improving manure nutrient management towards sustainable agricultural intensification in China

Theoretical and practical limitations of the acetylene inhibition technique to determine total denitrification losses

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