Biogenic amines mediate learning success in appetitive odor conditioning in honeybees

Raza, Muhammad Fahad; Wani, Tanweer; Liaqat, Zahid; Hussain, Naeem; Saed, Sattar; Husain, Arif; Hlaváč, Pavol; Kodrik, Milan; Ali, M. Ajmal; Rady, Ahmed; Su, Songkun

doi:10.1016/j.jksus.2022.101928

Cited by 8 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Improved learning success and might regulate optimal motivational or attentional levels (Raza et al, 2022).…”

Section: Apis Melliferamentioning

confidence: 99%

Invasive ant learning is not affected by seven potential neuroactive chemicals

Galante

Czaczkes

2022

Preprint

View full text Add to dashboard Cite

Nectar-feeding insects are often the victims of psychoactive manipulation, with plants lacing their nectar with secondary metabolites such as alkaloids and non-protein amino acids which often boost learning, foraging, or recruitment. However, the effect of neuroactive chemicals has seldomly been explored in ants. Argentine ants (Linepithema humile) are one of the most damaging invasive alien species worldwide. Enhancing or disrupting cognitive abilities, such as learning, has the potential to improve management efforts, for example by increasing preference for a bait, or improving ants' ability to learn its characteristics or location. Here, we test the effects of seven potential neuroactive chemicals - two alkaloids: caffeine and nicotine; two biogenic amines: dopamine and octopamine, and three non-protein amino acids: β-alanine, GABA and taurine - on the cognitive abilities of invasiveL. humileusing bifurcation mazes. Our results confirm that these ants are strong associative learners, requiring as little as one experience to develop an association. However, we show no short-term effect of any of the chemicals tested on spatial learning, and in addition no effect of caffeine on short-term olfactory learning. This lack of effect is surprising, given the extensive reports of the tested chemicals affecting learning and foraging in bees. This mismatch could be due to the heavy bias towards bees in the literature, a positive result publication bias, or differences in methodology.

show abstract

“…Improved learning success and might regulate optimal motivational or attentional levels (Raza et al, 2022).…”

Section: Apis Melliferamentioning

confidence: 99%

Invasive ant learning is not affected by seven potential neuroactive chemicals

Galante

Czaczkes

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The research suggests that honey bees less than 10 days old show a poorer performance in the PER training tests [34]. In our investigation, all the experimental bees were 12 days of age, did not become foragers, and were suitable for performing the PER experiments upon [54].…”

Section: Discussionmentioning

confidence: 60%

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Huang

Wang

Qiu

et al. 2023

Genes

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) play a vital role in the nerve regulation of honey bees (Apis mellifera). This study aims to investigate the differences in expression of miRNAs in a honey bee’s brain for olfactory learning tasks and to explore their potential role in a honey bee’s olfactory learning and memory. In this study, 12 day old honey bees with strong and weak olfactory performances were utilized to investigate the influence of miRNAs on olfactory learning behavior. The honey bee brains were dissected, and a small RNA-seq technique was used for high-throughput sequencing. The data analysis of the miRNA sequences revealed that 14 differentially expressed miRNAs (DEmiRNAs) between the two groups, strong (S) and weak (W), for olfactory performance in honey bees were identified, which included seven up-regulated and seven down-regulated. The qPCR verification results of the 14 miRNAs showed that four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory learning and memory. The target genes of these DEmiRNAs were subjected to the GO database annotation and KEGG pathway enrichment analyses. The functional annotation and pathway analysis showed that the neuroactive ligand-receptor interaction pathway, oxidative phosphorylation, biosynthesis of amino acids, pentose phosphate pathway, carbon metabolism, and terpenoid backbone biosynthesis may be a great important pathway related to olfactory learning and memory in honey bees. Our findings together further explained the relationship between olfactory performance and the brain function of honey bees at the molecular level and provides a basis for further study on miRNAs related to olfactory learning and memory in honey bees.

show abstract

“…Biogenic amines have been implicated in the regulation of behavior, and they may affect sensory perception 46 , 87 – 90 . We therefore screened our lists of brain and antennal DEGs for biogenic amine pathway genes.…”

Section: Resultsmentioning

confidence: 99%

Task-specific odorant receptor expression in worker antennae indicates that sensory filters regulate division of labor in ants

Caminer,

Libbrecht,

Majoe

et al. 2023

Commun Biol

View full text Add to dashboard Cite

Division of labor (DOL) is a characteristic trait of insect societies, where tasks are generally performed by specialized individuals. Inside workers focus on brood or nest care, while others take risks by foraging outside. Theory proposes that workers have different thresholds to perform certain tasks when confronted with task-related stimuli, leading to specialization and consequently DOL. Workers are presumed to vary in their response to task-related cues rather than in how they perceive such information. Here, we test the hypothesis that DOL instead stems from workers varying in their efficiency to detect stimuli of specific tasks. We use transcriptomics to measure mRNA expression levels in the antennae and brain of nurses and foragers of the ant Temnothorax longispinosus. We find seven times as many genes to be differentially expressed between behavioral phenotypes in the antennae compared to the brain. Moreover, half of all odorant receptors are differentially expressed, with an overrepresentation of the 9-exon gene family upregulated in the antennae of nurses. Nurses and foragers thus apparently differ in the perception of their olfactory environment and task-related signals. Our study supports the hypothesis that antennal sensory filters predispose workers to specialize in specific tasks.

show abstract

Biogenic amines mediate learning success in appetitive odor conditioning in honeybees

Cited by 8 publications

References 35 publications

Invasive ant learning is not affected by seven potential neuroactive chemicals

Invasive ant learning is not affected by seven potential neuroactive chemicals

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Task-specific odorant receptor expression in worker antennae indicates that sensory filters regulate division of labor in ants

Contact Info

Product

Resources

About