Biogenic Amines in<i>Drosophila virilis</i>under Stress Conditions

Hirashima, Akinori; Sukhanova, Madina; Rauschenbach, I. Yu.

doi:10.1271/bbb.64.2625

Cited by 81 publications

(64 citation statements)

References 30 publications

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“…Octopamine is often loosely characterised as a 'stress hormone' (Davenport and Evans, 1984a;Birmingham and Tauck, 2003), and levels have been shown to increase in the haemolymph by up to eightfold in response to conditions such as food deprivation (Davenport and Evans, 1984b) or heat stress (Hirashima et al, 2000). Indeed, the widespread changes in brain chemicals we report here, whilst clearly correlated with both solitarization and gregarization, do not necessarily have a causative role.…”

Section: Rapid Changes On Crowding or Isolationmentioning

confidence: 54%

Substantial changes in central nervous system neurotransmitters and neuromodulators accompany phase change in the locust

Rogers

Matheson

Sasaki

et al. 2004

Journal of Experimental Biology

123

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SUMMARY Desert locusts (Schistocerca gregaria) can undergo a profound transformation between solitarious and gregarious forms, which involves widespread changes in behaviour, physiology and morphology. This phase change is triggered by the presence or absence of other locusts and occurs over a timescale ranging from hours, for some behaviours to change, to generations,for full morphological transformation. The neuro-hormonal mechanisms that drive and accompany phase change in either direction remain unknown. We have used high-performance liquid chromatography (HPLC) to compare amounts of 13 different potential neurotransmitters and/or neuromodulators in the central nervous systems of final instar locust nymphs undergoing phase transition and between long-term solitarious and gregarious adults. Long-term gregarious and solitarious locust nymphs differed in 11 of the 13 substances analysed: eight increased in both the brain and thoracic nerve cord (including glutamate,GABA, dopamine and serotonin), whereas three decreased (acetylcholine,tyramine and citrulline). Adult locusts of both extreme phases were similarly different. Isolating larval gregarious locusts led to rapid changes in seven chemicals equal to or even exceeding the differences seen between long-term solitarious and gregarious animals. Crowding larval solitarious locusts led to rapid changes in six chemicals towards gregarious values within the first 4 h(by which time gregarious behaviours are already being expressed), before returning to nearer long-term solitarious values 24 h later. Serotonin in the thoracic ganglia, however, did not follow this trend, but showed a ninefold increase after a 4 h period of crowding. After crowding solitarious nymphs for a whole larval stadium, the amounts of all chemicals, except octopamine, were similar to those of long-term gregarious locusts. Our data show that changes in levels of neuroactive substances are widespread in the central nervous system and reflect the time course of behavioural and physiological phase change.

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Section: Rapid Changes On Crowding or Isolationmentioning

confidence: 54%

Substantial changes in central nervous system neurotransmitters and neuromodulators accompany phase change in the locust

Rogers

Matheson

Sasaki

et al. 2004

Journal of Experimental Biology

123

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“…The increase in DA content is followed by a sharp decrease in the activity of the key enzyme of its synthesis, tyrosine hydroxylase (TH), and this decrease continues for up to 60 min after the onset of stress exposure (Rauschenbach et al, 1995b). A similar pattern is observed with the OA system, the only difference being that the decrease in activity of tyrosine decarboxylase, the first enzyme involved in OA synthesis, continues for up to 240 min of the stress exposure Hirashima et al, 2000b). The activity of alkaline phosphatase [ALP, the enzyme that regulates the pool of the DA and OA precursor tyrosine (Wright, 1987)] also decreases abruptly under stress conditions (Sukhanova et al, 1996).…”

Section: Introductionmentioning

confidence: 78%

“…In these species, DA content increases sharply as early as 15 min after the beginning of stress exposure (38°C) (Rauschenbach et al, 1993;Hirashima et al, 2000b;Gruntenko et al, 2004). The increase in DA content is followed by a sharp decrease in the activity of the key enzyme of its synthesis, tyrosine hydroxylase (TH), and this decrease continues for up to 60 min after the onset of stress exposure (Rauschenbach et al, 1995b).…”

Section: Introductionmentioning

confidence: 99%

Disruption of insulin signalling affects the neuroendocrine stress reaction in Drosophila females

Rauschenbach

Karpova

Adonyeva

et al. 2014

Journal of Experimental Biology

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Juvenile hormone (JH) and dopamine are involved in the stress response in insects. The insulin/insulin-like growth factor signalling pathway has also recently been found to be involved in the regulation of various processes, including stress tolerance. However, the relationships between the JH, dopamine and insulin signalling pathways remain unclear. Here, we study the role of insulin signalling in the regulation of JH and dopamine metabolism under normal and heat stress conditions in Drosophila melanogaster females. We show that suppression of the insulin-like receptor (InR) in the corpus allatum, a specialised endocrine gland that synthesises JH, causes an increase in dopamine level and JH-hydrolysing activity and alters the activities of enzymes that produce as well as those that degrade dopamine [alkaline phosphatase (ALP), tyrosine hydroxylase (TH) and dopamine-dependent arylalkylamine N-acetyltransferase (DAT)]. We also found that InR suppression in the corpus allatum modulates dopamine, ALP, TH and JH-hydrolysing activity in response to heat stress and that it decreases the fecundity of the flies. JH application restores dopamine metabolism and fecundity in females with decreased InR expression in the corpus allatum. Our data provide evidence that the insulin/insulin-like growth factor signalling pathway regulates dopamine metabolism in females of D. melanogaster via the system of JH metabolism and that it affects the development of the neuroendocrine stress reaction and interacts with JH in the control of reproduction in this species.

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“…Tyrosine acts as a precursor of several stress hormones in insects, including dopamine, octopamine, and tyramine (19,37). In the present study, we found that tyrosine was markedly elevated in the S flies, whereas the levels were intermediate in the H flies and close to control in the HS flies.…”

Section: Fig 4 Dendrograms (A-c) and Pls-da Plots (D-f) Showing Simmentioning

confidence: 99%

Metabolomic profiling of heat stress: hardening and recovery of homeostasis in Drosophila

Malmendal¹,

Overgaard²,

Bundy³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

185

135

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Frequent exposure of terrestrial insects to temperature variation has led to the evolution of protective biochemical and physiological mechanisms, such as the heat shock response, which markedly increases the tolerance to heat stress. Insight into such mechanisms has, so far, mainly relied on selective studies of specific compounds or characteristics or studies at the genomic or proteomic levels. In the present study, we have used untargeted NMR metabolomic profiling to examine the biological response to heat stress in Drosophila melanogaster. The metabolite profile was analyzed during recovery after exposure to different thermal stress treatments and compared with untreated controls. Both moderate and severe heat stress gave clear effects on the metabolite profiles. The profiles clearly demonstrated that hardening by moderate heat stress led to a faster reestablishment of metabolite homeostasis after subsequent heat stress. Several metabolites were identified as responsive to heat stress and could be related to known physiological and biochemical responses. The time course of the recovery of metabolite homeostasis mirrored general changes in gene expression, showing that recovery follows the same temporal pattern at these two biological levels. Finally, our data show that heat hardening permits a quicker return to homeostasis, rather than a reduction of the acute metabolic perturbation and that the reestablishment of homeostasis is important for obtaining maximal heat-hardening effect. The results display the power of NMR metabolomic profiling for characterization of the instantaneous physiological condition, enabling direct visualization of the perturbation of and return to homeostasis. metabolomics; heat shock protein; nuclear magnetic resonance spectroscopy; insect ECTOTHERMIC ANIMALS EXPERIENCE fluctuating temperatures on both a daily and seasonal scale. In some cases, these fluctuations may become severely stressful or even lethal, and so these animals possess a number of physiological, biochemical, or behavioral adaptations that allow them to overcome such stressful situations. For instance, the fruitfly (Drosophila melanogaster) has the capacity to survive transient exposures to temperatures ranging from less than Ϫ10°C to above 40°C, and hence this species has been widely used as a model to investigate different aspects of the temperature stress response (21).Physiological stress can have effects on many biological levels. Because metabolites are downstream of both gene transcripts and proteins, changes in metabolite levels can provide an indication of the overall integrated response of an organism. To obtain a better understanding of the effects of heat stress on metabolite concentrations, it is relevant to adopt an integrative approach, such as simultaneous measurement of the response to stress situations of all metabolites present (above a concentration threshold) in the organism, e.g., by NMR spectroscopy. This approach, termed metabolomics, is a holistic approach complementary to genomics and prote...

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Biogenic Amines inDrosophila virilisunder Stress Conditions

Cited by 81 publications

References 30 publications

Substantial changes in central nervous system neurotransmitters and neuromodulators accompany phase change in the locust

Substantial changes in central nervous system neurotransmitters and neuromodulators accompany phase change in the locust

Disruption of insulin signalling affects the neuroendocrine stress reaction in Drosophila females

Metabolomic profiling of heat stress: hardening and recovery of homeostasis in Drosophila

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