Biogenic amines and β-endorphin responses in the rat vas deferens

Miranda, Hugo F.; Vergara, Jorge; Rosselot, F.; Fernàndez, E.

doi:10.1016/0306-3623(82)90009-x

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…The results obtained with the bioassay used in the present investigation led us to postulate that gonadectomy and/or adrenal ectomy were not able to alter the capacity of (3-endorphin to activate a specific opioid re ceptor present in the rat vas deferens, the so called 'e-receptor' [Miranda et al, 1979;Wuster et al, 1979;Schulz et al, 1979;Was ter et al, 1981;Miranda et al, 1982], Ac cording to the data obtained from the iso lated rat vas deferens that was electrically stimulated, changes in the sensitivity and reactivity to (3-cndorphin were not delected.…”

Section: Discussionmentioning

confidence: 92%

Androgens and Effects of Opioids and Catecholamines on the Smooth Muscle of the Rat Vas deferens

Miranda¹,

Pérez

Fernàndez

et al. 1985

Pharmacology

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In the neuronal electrically stimulated vas deferens of the rat, the responses evoked by morphine, β-endorphin and catecholamines were studied. The tissue was obtained from rats at various times (7–60 days) following castration, adrenalectomy and castration with adrenalectomy. Pharmacological data are presented which verify an increased reponsiveness to morphine after castration with or without adrenalectomy. In contrast, decreased responsiveness was observed after adrenalectomy alone. Decreased responsiveness of the vas deferens to catecholamines was observed after castration, adrenalectomy and castration with adrenalectomy. Since the responsiveness of the electrically stimulated vas deferens to β-endorphin was unchanged by this treatment (castration, adrenalectomy), it is suggested that the ‘ε-opioid’ receptor is unaffected by androgens.

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Section: Discussionmentioning

confidence: 92%

Androgens and Effects of Opioids and Catecholamines on the Smooth Muscle of the Rat Vas deferens

Miranda¹,

Pérez

Fernàndez

et al. 1985

Pharmacology

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Presynaptic Regulation by ACh of the NE Mediated Responses in the Rat Vas Deferens

Miranda¹,

Wolstenholme

1985

Journal of Receptor Research

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In the isolated electrically stimulated vas deferens preparation the effect of exogenous acetylcholine (ACh) was studied. It was possible to differentiate two separate sites for the action of ACh. A postsynaptic effect (M1) which is revealed as a sudden decrease in the basal tension of the muscular twitch, antagonized competitively by atropine (pA2 = 8.44 +/- 0.79), potentiated at a ratio of 10.24 by neostigmine, and not altered by hexamethonium, yohimbine, clonidine, theophylline or prazosin. Treatment with reserpine or 6-OH-DA induced a supersensitivity of this effect. The second action is a presynaptic effect (M2), which is manifested by a rapid increase in the muscular twitch, is dose-dependent to ACh, and is potentiated significantly by reserpine, neostigmine and clonidine. This latter effect was not modified by atropine, hexamethonium, yohimbine, prazosin or theophylline. The normal efficacy of ACh is 185 times greater in presynaptic over postsynaptic receptors. The increase in the twitch is not considered to be by the release of endogenous ACh or ATP. The evidence presented here indicates that the presynaptic effect of ACh (M2) is due to participation of alpha 2-adrenoceptors in enhancement of the release of norepinephrine (NE) from the nerve terminals.

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Biogenic amines and β-endorphin responses in the rat vas deferens

Cited by 2 publications

References 26 publications

Androgens and Effects of Opioids and Catecholamines on the Smooth Muscle of the Rat Vas deferens

Androgens and Effects of Opioids and Catecholamines on the Smooth Muscle of the Rat Vas deferens

Presynaptic Regulation by ACh of the NE Mediated Responses in the Rat Vas Deferens

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