Biogenetic Acellular Dermal Matrix Maintaining Rich Interconnected Microchannels for Accelerated Tissue Amendment

Abstract

Given that many people suffer from extensive skin damage, wound repair has drawn tremendous attention in research. Among the various assistant dressing materials that promote healing, a porcine acellular dermal matrix (PADM), as a skin substitute, can efficiently accelerate healing by promoting cell migration and proliferation. However, a simple, low-cost preparation process remains a challenge facing PADM development, particularly because of the inferior elasticity. To overcome these drawbacks, a CaCl2–ethanol–H2O solution (ternary solution) combined with an additional enzyme treatment was used to obtain a transparent, porous, and elastic PADM that retained the major extracellular matrix composition of the dermis. Our results indicated that alterations in the fiber organization and secondary structural changes in the collagen occurred after treatment. Furthermore, the in vivo wound healing and histological analyses clearly revealed an extremely expedited wound repair process following the application of the biocompatible PADM. In conclusion, this study provides new insights into the development of a transparent PADM with a porous structure and good elasticity that can be used as a skin substitute to accelerate the wound healing process. Moreover, this effective technique could potentially be used to extrapolate other decellularized materials in the future.

Bioprinting a skin patch with dual-crosslinked gelatin (GelMA) and silk fibroin (SilMA): An approach to accelerating cutaneous wound healing

“…Currently, three-dimensional (3D) bioprinting has emerged as an efficient technique for the healing of cutaneous wounds [ [5] , [6] , [7] ]. As a result of its accessibility, cost-effectiveness and ability to reproduce tissue complexity, extrusion-based bioprinting integrated with cell-loaded hydrogel has become the most suitable technique for treating cutaneous wounds.…”

Heparinized Collagen Scaffolds Based on Schiff Base Bonds for Wound Dressings Accelerate Wound Healing without Scar

“…10 pADM also contains bioactive macromolecules such as growth factors and glycosaminoglycans. 11 It can regulate the extracellular matrix microenvironment during wound healing and stimulate the growth of granulation tissue in wounds. While accelerating wound healing, it can also reduce the scarring and other problems caused by the lack of dermal tissue during the healing process of deeply injured wounds.…”

“…It can provide physical support for cell growth and guide cell infiltration, proliferation, differentiation, and migration . pADM also contains bioactive macromolecules such as growth factors and glycosaminoglycans . It can regulate the extracellular matrix microenvironment during wound healing and stimulate the growth of granulation tissue in wounds.…”

Membranous Extracellular Matrix-Based Scaffolds for Skin Wound Healing

“…Although the use of traditional ECM membranes for skin wound healing can go back decades, new types of decellularized membranes, such as those produced from forestomach, chorion, pleura, and peritoneum, are emerging [20,59,174]. The development of new convenient cell extraction strategies, such as the use of supercritical carbon dioxide for scaffold decellularization, has been reported in the literature [175][176][177][178]. This progress offers more technical options and raw ECM materials for the development of new wound dressings.…”