Biogenesis of mammalian mitochondria in the renoprival kidney

Factors Affecting Protein and Glycoprotein Synthesis by Rat Kidney Mitochondria <i>in vitro</i>

“…All animals survived and appeared healthy when sacrificed 24 h after surgery. Total activity for liCleucine and llC-mannose incorporation ex pressed as mitochondria per kidney or per gram kidney wet weight was increased in the uninephrectomized animals compared to sham-operated controls thereby confirming in part the results of Devlin and Cli'ill [3] (table I). The increase in specific activity, CPM/mg protein, for 14C-leucine incorporation by mitochondria following uninephrectomy re ported by the latter investigators was not ob served.…”

“…Alterations in mitochondrial macromolecular synthesis may be a feature of various types of renal injury though the importance of mitochondrial biosynthetic activity to the overall cell response remains to be established. inhibition of intrinsic mitochondrial pro tein synthesis leads to structural and functional alterations of these organelles [5,17], Inas much as mitochondrial protein synthesis is required for the formation of new mitochon dria and is enhanced during the compensatory hyperplastic response which follows uninephrcctomy [3], factors interfering with this process may limit biogenesis and decrease the cellular mitochondrial population. In view of…”

“…In vitro protein synthesis by mitochondria can be assayed by measuring the incorporation of radioactively labeled amino acids [1,3,5,11], In parallel with studies on the effect of in jected nephrotoxins on mitochondrial protein synthesis, we have evaluated the influence of several compounds on amino acid and carbo hydrate incorporation by isolated mitochon dria. The results indicate the potential for cer tain acknowledged nephrotoxins to impair macromolecular synthesis by mitochondria in vitro.…”

Cycloheximide as a probe of fibrinogen synthesis

“…Supernatants of the fractions from which ribonucleoprotein complexes were isolated were titrated with anti-fibrinogen, and, at the equivalence point, precipitated antibody-fibrinogen complexes were collected by centrifugation (5000g for 10min). Radioactivity was determined as described by Devlin & Ch'ih (1974). Protein was measured by the method of Lowry et al (1951), with bovine serum albumin as standard.…”

Alterations of Mitochondrial Properties in Folate Nephropathy

“…Stimu-lation of renal mitochondrial protein synthesis has been documented by 24 h after uninephrectomy [7] and may be essential for the increase in the number of mitochondria per cell reported by Cuppage et ai. [6] to occur during the first 3 days after uninephrectomy.…”