Biogenesis of Gold Nanoparticles Using Plant Powders and Assessment of In Vitro Cytotoxicity in 3T3-L1 Cell Line

Kalpana, Duraisamy; Pichiah, P.B. Tirupathi; Arunachalam, Sankarganesh; Park, Whoa Shig; Lee, Seok Myon; Wahab, Rizwan; Soo, Youn; Lee, Yang Soo

doi:10.1007/s12247-013-9166-x

Cited by 14 publications

(3 citation statements)

References 42 publications

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“…17 The TEM micrographs of GA-AuNPs proved its shape as spherical with sizes <55 nm. Our results were corroborated with the nding of Kalpana et al 18 who have observed the spherical morphology with sizes <80 nm for the plant powder reduced gold nanoparticles. The size and shape of nanoparticles are mostly determined by various factors such as the nature of plant extract and its concentration, metal salt, pH, temperature, extent of reaction time and the mixing ratio of plant extract and metal salt.…”

Section: Discussionsupporting

confidence: 92%

Synthesis and characterization of biocompatible gymnemic acid–gold nanoparticles: a study on glucose uptake stimulatory effect in 3T3-L1 adipocytes

Rajarajeshwari¹,

Shivashri²,

Rajasekar³

2014

RSC Adv.

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Section: Discussionsupporting

confidence: 92%

Synthesis and characterization of biocompatible gymnemic acid–gold nanoparticles: a study on glucose uptake stimulatory effect in 3T3-L1 adipocytes

Rajarajeshwari¹,

Shivashri²,

Rajasekar³

2014

RSC Adv.

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“…Mishra et al (2013) [53] found that, at a concentration of 2.5 ng/mL, gold nanoparticles (size range of 10–60 nm with near spherical morphology) synthesised using the extract of Hibiscus sabdariffa caused a decrease in viability of ∼30 and 95% in 293 normal cells and U87 GBM malignant cells, respectively, after 48 h of exposure. Gold nanoparticles (range of 8–42 nm) synthesised using Torreya nucifera at a concentration of 1 ng/mL showed cytotoxicity of 14.85% to 3T3‐L1 cells after exposure for 24 h [54]. Valodkar et al (2011b) [55] showed that silver NPs synthesised using latex from E. nivulia were cytotoxic to A549 cells in a dose‐dependent manner and that this cytotoxicity was related to the internalisation of these NPs and generation of oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

Green synthesis of colloidal gold nanoparticles using latex from Hevea brasiliensis and evaluation of their in vitro cytotoxicity and genotoxicity

Santos

Gomes

Cavalcante

et al. 2019

IET nanobiotechnol.

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Latex extracted from Hevea brasiliensis tree has been used as a green alternative for preparing gold nanoparticles (Au NPs); however, no study evaluating the cytotoxic and genotoxic potential of Au NPs synthesised using H. brasiliensis has been published. The present study aimed to synthesise and characterise colloidal Au NPs using latex from H. brasiliensis and to evaluate their in vitro cytotoxicity and genotoxicity. Ideal conditions for the green synthesis of Au NPs were studied. In vitro cytotoxicity and genotoxicity of Au NPs in CHO-K1 cells was also evaluated. Our findings indicated that the ideal synthesis conditions of pH, temperature, reduction time, and concentrations of latex and HAuCl 4 were 7.0, 85°C, 120 min, 3.3 mg/mL, and 5.0 mmol/L, respectively. LC50 24 h of Au NPs was 119.164 ± 5.31 μg/mL. Lowest concentration of Au NPs tested presented minimal cytotoxicity and genotoxicity. However, high concentrations of Au NPs promoted DNA damage and cell death via apoptosis. On the basis of these findings, the authors optimised the use of an aqueous solution of H. brasiliensis latex as a reducing/stabilising agent for the green synthesis of Au NPs. Low concentrations of these NPs are biocompatible in normal cell types, suggesting that these NPs may be used in biological applications.

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“…Gold nanoparticles prepared by combining three different leaf powders of T. nucifera , C. japonicum , and N. indicum were also evaluated on 3T3-L1 cell line. Particles absorption was remarkable but the cells exhibited some dose dependent cytotoxicity [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs

Alhaji

Chowdhury

Rosli

et al. 2014

BioMed Research International

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Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells. Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs. This study aimed to (1) assess the gene delivery efficiency of CO3Ap in vitro and in mouse lungs, (2) evaluate the cytotoxicity effect of CO3Ap/pDNA in vitro, and (3) characterize the CO3Ap/pDNA complex formulations. A significantly high level of reporter gene expression was detected from the lung cell line transfected with CO3Ap/pDNA complex prepared in both serum and serum-free medium. Cytotoxicity analysis revealed that the percentage of the viable cells treated with CO3Ap to be almost similar to the untreated cells. Characterization analyses showed that the CO3Ap/pDNA complexes are in a nanometer range with aggregated spherical structures and tended to be more negatively charged. In the lung of mice, highest level of transgene expression was observed when CO3Ap (8 μL) was complexed with 40 μg of pDNA at day 1 after administration. Although massive reduction of gene expression was seen beyond day 1 post administration, the level of expression remained significant throughout the study period.

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Biogenesis of Gold Nanoparticles Using Plant Powders and Assessment of In Vitro Cytotoxicity in 3T3-L1 Cell Line

Cited by 14 publications

References 42 publications

Synthesis and characterization of biocompatible gymnemic acid–gold nanoparticles: a study on glucose uptake stimulatory effect in 3T3-L1 adipocytes

Synthesis and characterization of biocompatible gymnemic acid–gold nanoparticles: a study on glucose uptake stimulatory effect in 3T3-L1 adipocytes

Green synthesis of colloidal gold nanoparticles using latex from Hevea brasiliensis and evaluation of their in vitro cytotoxicity and genotoxicity

Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs

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