Biogenesis and Properties of the Mitochondrial Cyanide-Insensitive Alternative Respiratory Pathway in the Yeast Saccharomycopsis Lipolytica

Henry, Michèle-France; Troostembergh, Jean‐Claude De; Nyns, Edmond‐Jacques

doi:10.1016/b978-0-08-022630-9.50011-6

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…Two alternative antimycin-insensitive oxidase pathways have been described for a number of yeasts (Lloyd, 1974;Goffeau & Crosby, 1978;Henry et al, 1978), and the observations with inhibitors, described in this paper, indicate that Kluyveromyces fragilis shows a similar oxidase pathway to that reported for Kluyveromyces Zactis (Ferrero et al, 1981). Clearly, such alternative pathways (here in lactose-grown yeast) can support active transport, although at a reduced rate, and giving only about a 10-fold concentration of the exogenous substrate, compared with at least 50-fold for yeast free of antimycin.…”

Section: Energetics Of Aerobic Transportsupporting

confidence: 59%

The Requirement of Oxygen for the Active Transport of Sugars into Yeasts

Barnett

Sims

1982

Microbiology

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The inducible systems of uptake of P-D-galactopyranosides into Kluyveromyces fragilis, Kluyveromyces marxianus and Debaryumyces polymorphus were studied under aerobic and anaerobic conditions, using both lactose and the non-metabolized analogue, methyl 1 -thio-P-D-galactopyranoside (TMG). A common carrier served both substrates, and aerobic entry was by active transport, involving proton symport. The rate of uptake and the final equilibrium concentration were decreased on adding N,N'-dicyclohexylcarbodiimide, diethylstilbestrol or antimycin A, and active transport was completely abolished by carbonyl cyanide m-chlorophenylhydrazone. Uptake under anaerobic conditions differed markedly from that occurring aerobically : TMG was not concentrated anaerobically, even by strongly fermenting yeasts. Instead, it was transported into the cells by facilitated diffusion, which could sustain a rate of entry of over half the maximum rate observed aerobically. That this difference between aerobic and anaerobic transport into yeasts might apply to glycosides in general, was suggested by the finding that a strain of Saccharomyces cerevisiae also took up maltose by active transport under aerobic conditions, but by facilitated diffusion anaerobically. By contrast, the amino acid, 2-aminoisobutyric acid, was concentrated by Kluyveromyces fragilis, even under anaerobic conditions. The significance of these findings in relation to the Kluyver effect is discussed.

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Section: Energetics Of Aerobic Transportsupporting

confidence: 59%

The Requirement of Oxygen for the Active Transport of Sugars into Yeasts

Barnett

Sims

1982

Microbiology

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“…Computer simulation of the effect of diffusion barriers on the respiratory oxygen isotherm in avocado fruit predicts that the apparent K^ measured at the surface of a freely accessible cell for the high affinity oxygen response would be approximately 3.0 mmol m~^. This value is to be compared with an apparent K^ at the cell surface of 3.8 mmol m^^ reported for cytoehrome oxidase in yeast (Henry et at., 1977). Thus, it is concluded that the apparent K^ for the high affinity oxygen response in avocado reflects the affinity for oxygen of cytoehrome oxidase.…”

Section: Basis Of the Biphasie Isothermmentioning

confidence: 91%

“…It was neeessary to measure oxygen isotherms at reduced pressure because as noted above, diffusion barriers otherwise make it diflicult to distinguish between two oxidases with a relatively high affinity for oxygen [e.g. A:,,,'S of 3.8 and 11.5 mmol m~' for whole yeast cytochrotne oxidase and alternative oxidase, respectively (Henry, de Troostembergh & Nyns, 1977)]. At reduced pressure the effect of diffusion barriers is minimized, and it becomes possible to distinguish between the two oxidases.…”

Section: (Cl -C2) = [{Q/4np){\/r^)]mentioning

confidence: 99%

“…Thus, it is concluded that the apparent K^ for the high affinity oxygen response in avocado reflects the affinity for oxygen of cytoehrome oxidase. Kinetic analysis of respiratory isotherms of preclimacteric and climacteric avocado fruit both at 25 and 101 KPa in the presence and absence of cyanide indicate that the cyanide-resistant alternative path, which has a lower affinity for oxygen than does cytoehrome oxidase (Solomos, 1977;Henry et al, 1977), does not contribute appreciably to fruit respiration in the absence of the inhibitor.…”

Section: Basis Of the Biphasie Isothermmentioning

confidence: 99%

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The dual role of oxygen in avocado fruit respiration: Kinetic analysis and computer modelling of diffusion‐affected respiratory oxygen isotherms

Tucker

Laties

1985

Plant Cell & Environment

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Oxygen plays a dual role in affecting the rate of respiration of avocado fruit {Pcrsca atncricana Mill, cv. Hass). The respiration rate 1' . oxygen concentr-ation cut-vc for steady state avocado fruit respiration is biphasic. The curve becomes monophasic, however, when measured under conditions of rapidly changing oxygen concentration in a closed circulating system. The results are interpreted as indicating that oxygen at relatively high concentrations modulates respiration independent of its interaction with the terminal oxidasepresutnably cytochrome oxidase.A cotnputer model is presented which takes into account the effect of diffusion barriers on the kinetics of oxygen utilization as a function of concentration in avocado fruit. The model is used to tnake predictions concerning the apparent A^,,, of the terminal oxidase or oxidases in avocado fruit. It is concluded Ihat Ihe apparent A^, , , of the terminal oxidase of uninhibited avocado fruit is that of eytochrome oxidase, and that the alternative, eyanidc-resistant oxidase of avocado fruit does not contribute appreciably to the uninhibited respiration of preclin-iacteric or climacteric avocado fruit,

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