“…Therefore, the biochemical processes include disintegration from homogeneous particulates to carbohydrates, proteins and lipids, extracellular hydrolysis of these particulate substrates to sugars, amino acids, and long-chain fatty acids, acidogenesis from sugars and amino acids to volatile fatty acids and hydrogen, acetogenesis of long-chain fatty acids and volatile fatty acids to acetate, and methanogenesis from acetate and hydrogen, Fig. 1 (Batstone et al 2002;Scholwin et al 2009). The composition and location of the wax layer makes its conversion into methane by these enzymes likely to be easier and faster than the lignocellulosic parts of the plant material for two reasons: first, the components of the wax layer are builtup of quite small molecules, which allow their conversion into methane without depolymerisation, and second, they are not linked to lignin (Tong and McCarty 1991).…”