IntroductionJusticia schimperiana (Hochst.ex A. Nees) T. Anders is a perennial herb or shrub with many branched stems, up to 0.8-3 m tall with slightly unpleasant smell. Leaves blade up to 15-24 × 8-12 cm, ovateelliptic, broadest near the base, base cuneate to attenuate entire along the margins, apex acuminate, pubescent along veins on both surfaces, or rarely pubescent all over both surfaces; lateral veins 5-9 pairs; petiole up to 10-40 mm long; pubescent with non-glandular hairs to nearly glabrous [1,2]. The Ethiopian government under NBPE in collaboration with the Netherland Development Organization (SNV Ethiopia), an international NGO, has embarked on an ambitious biogas program to construct biogas plants to address the rural energy crisis and indoor pollution caused by the burning of traditional biomass. The first phase program is being implemented in Amhara, SNNPRS, Oromia and Tigray regions [9]. However, the only targeted raw material being used for the biogas production is manure which has lower energy content than green shrubs since animals that produce it have already digested the substrate [10,11]. So, co-digestion using leaves of JS could be important for sustainable and uniform feeding of the digesters and therefore relatively higher biogas production and slurry use. It may also open the opportunity for those households who have less than four heads of cattle to be included in the program [12]. This study is