Biogas production and microbial community properties during anaerobic digestion of corn stover at different temperatures

Liu, Chunmei; Wachemo, Akiber Chufo; Tong, Huan; Shi, SiHui; Zhang, Liang; Yuan, Hairong; Li, Xiujin

doi:10.1016/j.biortech.2017.12.076

Cited by 129 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The pattern of SH resembled that of CH. In this case three putative species of the genus Methanobacterium represented more than 78% of the community (Barret et al, 2013;Wintsche et al, 2016;Liu et al, 2018). The diversity of sample CNw12 was strikingly low.…”

Section: T-rflp Analysis Of Archaeal Mcra Genementioning

confidence: 87%

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

et al. 2019

View full text Add to dashboard Cite

The biological conversion of hydrogen (H 2) and carbon dioxide (CO 2) to methane (CH 4), is accomplished by the hydrogenotrophic methanogens (HM). HMs are difficult to cultivate in pure culture, but they are readily available in the mixed culture of effluents from the anaerobic degradation of organic matter, i.e., the fermentation effluent of biogas plants. The rate-limiting step in the work of CH 4-forming microbial communities is the low solubility of H 2 in the aqueous environment. In our approach, the simple fed-batch fermentation technique was selected to supply the gaseous substrates for the microbial community at laboratory scale and mesophilic temperature. Periodically withdrawn samples were analyzed for process parameters and the microbial communities were studied using Terminal Restriction Fragment Length Polymorphism (T-RFLP) of the mcrA gene and Ion Torrent whole metagenome DNA sequencing. The metagenome data were evaluated by both read-based and genome-centric bioinformatics tools. The rearrangements in the mixed microbial communities, triggered by switching the operating conditions to biological power-to-biomethane (bio-P2M), have been established. The production rates were 6.30 mL CH 4 L −1 h −1 during the acclimation phase and 9.21 mL CH 4 L −1 h −1 by the fully adapted community, respectively. The diversity of the anaerobic microbiota decreased as the bio-P2M process progressed. Feeding the community with H 2 apparently promoted the abundance of several genera, in particular Candidatus Cloacimonas and Herbinix. The diversity of the Archaea community decreased considerably upon daily feeding with H 2 and CO 2. The predominant Archea genus was Methanobacterium in every reactor, Methanothrix persisted for the first 4 weeks, while the initially less abundant genus Methanoculleus gained advantage during the adaptation to the sustained bio-P2M process. The accumulation of acetate indicated a strong involvement of homoacetogenic bacteria.

show abstract

Section: T-rflp Analysis Of Archaeal Mcra Genementioning

confidence: 87%

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Therefore, in [13] optimal conditions for anaerobic digestion at thermophilic temperatures could be determined. Many studies confirm the increasing efficiency and degradation rate of the process with rising temperatures [14][15][16].…”

Section: Introductionmentioning

confidence: 88%

Effect of Different Operating Temperatures on the Biological Hydrogen Methanation in Trickle Bed Reactors

Lemmer

Ullrich

2018

Energies

View full text Add to dashboard Cite

To improve the reactor efficiency, this study investigated the influence of temperature on the biological hydrogen methanation (BHM) in trickle-bed reactors (TBR). Rising temperatures increase the metabolic activity of methanogenic microorganisms, thus leading to higher reactor specific methane formation rates (MFR). In order to quantify the potential for improved performance, experiments with four different operating temperatures ranging from 40 to 55 • C were carried out. Methane content increased from 88.29 ± 2.12 vol % at 40 • C to 94.99 ± 0.81 vol % at 55 • C with a stable biological process. Furthermore, a reactor specific methane formation rate (MFR) of up to 8.85 ± 0.45 m 3 m −3 d −1 was achieved. It could be shown that the microorganisms were able to adapt to higher temperatures within hours. The tests showed that TBR performance with regard to BHM can be significantly increased by increasing the operating temperature.

show abstract

“…In the effluent of each system, a positive correlation was observed between loading rates (volumetric, organic and solids) and the relative abundance of Firmicutes and Euryarchaeota in system effluent; however, no consistent relationship was observed between those phyla in the sludge of each system and loading rates (Supplementary Materials 5). In sludge samples from each system, strong positive correlations were observed between system temperature and the relative abundance of the phyla Firmicutes and Bacteroidetes (Supplementary Materials 5); organisms in these phyla are associated with hydrolysis in full-scale anaerobic digestion [32,33]. Thus, assuming that the microbial community in the retained sludge is predominantly responsible for driving treatment in each system, the capacity to increase temperature in the SST could enable improved breakdown of solids, as was observed in this study, by temperature-associated selection of those phyla.…”

Section: Correlating System Operation and Microbial Composition In Slmentioning

confidence: 99%

Solar Septic Tank: Next Generation Sequencing Reveals Effluent Microbial Community Composition as a Useful Index of System Performance

Connelly

Pussayanavin

Randle-Boggis

et al. 2019

Water

View full text Add to dashboard Cite

Septic tanks are widely deployed for off-grid sewage management but are typified by poor treatment performance, discharge of polluting effluents and the requirement for frequent de-sludging. The Solar Septic Tank (SST) is a novel septic tank design that uses passive heat from the sun to raise in-tank temperatures and improves solids degradation, resulting in a cleaner effluent. Treatment has been shown to exceed conventional systems, however, the underlying biology driving treatment in the system is poorly understood. We used next generation sequencing (Illumina Miseq (San Diego, CA, USA), V4 region 16S DNA) to monitor the microbiology in the sludge and effluent of two mature systems, a conventional septic tank and an SST, during four months of routine operation in Bangkok, Thailand, and evaluated the ecology against a suite of operating and performance data collected during the same time period. Significant differences were observed between the microbiome of the sludge and effluent in each system and the dominant taxa in each appeared persistent over time. Furthermore, variation in the microbial community composition in the system effluents correlated with effluent water quality and treatment performance parameters, including the removal of chemical and biochemical oxygen demand and the concentration of fecal and total coliforms in the effluent. Thus, we propose that a wide-scale survey of the biology underlying decentralised biotechnologies for sewage treatment such as the SST could be conducted by sampling system effluent rather than sampling sludge. This is advantageous as accessing sludge during sampling is both hazardous and potentially disruptive to the anaerobic methanogenic consortia underlying treatment in the systems.

show abstract

Biogas production and microbial community properties during anaerobic digestion of corn stover at different temperatures

Cited by 129 publications

References 34 publications

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

Effect of Different Operating Temperatures on the Biological Hydrogen Methanation in Trickle Bed Reactors

Solar Septic Tank: Next Generation Sequencing Reveals Effluent Microbial Community Composition as a Useful Index of System Performance

Contact Info

Product

Resources

About