Biofunctionalized PEDOT-coated microfibers for the treatment of spinal cord injury

Alves-Sampaio, Alexandra; García-Rama, Concepción; Collazos‐Castro, Jorge E.

doi:10.1016/j.biomaterials.2016.02.037

Cited by 48 publications

(55 citation statements)

References 73 publications

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“…A focus of this study was to develop a low‐temperature, simple imprinting method to reproduce lithographically fabricated well‐defined and ordered topographies into PEDOT:PTS electrodeposited electrode coatings. Critically this approach preserves the ability to introduce coupled biochemical functionalization—an approach which has been explored extensively with PEDOT functionalized microelectrodes . Arrays of pits possessing a diameter of ≈1 µm, a pitch of ≈2 µm, and depth of ≈1 µm were initially fabricated via photolithography, which was used as a mask for a Ni (Nickel) electroforming process as described previously .…”

Section: Resultsmentioning

confidence: 99%

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Vallejo-Giraldo

Krukiewicz

Calaresu

et al. 2018

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Following implantation, neuroelectrode functionality is susceptible to deterioration via reactive host cell response and glial scar-induced encapsulation. Within the neuroengineering community, there is a consensus that the induction of selective adhesion and regulated cellular interaction at the tissue-electrode interface can significantly enhance device interfacing and functionality in vivo. In particular, topographical modification holds promise for the development of functionalized neural interfaces to mediate initial cell adhesion and the subsequent evolution of gliosis, minimizing the onset of a proinflammatory glial phenotype, to provide long-term stability. Herein, a low-temperature microimprint-lithography technique for the development of micro-topographically functionalized neuroelectrode interfaces in electrodeposited poly(3,4-ethylenedioxythiophene):p-toluene sulfonate (PEDOT:PTS) is described and assessed in vitro. Platinum (Pt) microelectrodes are subjected to electrodeposition of a PEDOT:PTS microcoating, which is subsequently topographically functionalized with an ordered array of micropits, inducing a significant reduction in electrode electrical impedance and an increase in charge storage capacity. Furthermore, topographically functionalized electrodes reduce the adhesion of reactive astrocytes in vitro, evident from morphological changes in cell area, focal adhesion formation, and the synthesis of proinflammatory cytokines and chemokine factors. This study contributes to the understanding of gliosis in complex primary mixed cell cultures, and describes the role of micro-topographically modified neural interfaces in the development of stable microelectrode interfaces.

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Section: Resultsmentioning

confidence: 99%

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Vallejo-Giraldo

Krukiewicz

Calaresu

et al. 2018

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“… 13 In line with these findings, Collazos-Castro et al recently demonstrated the utility of carbon microfibers (7 μm in diameter) coated with PEDOT and biofunctionalized (by using a multimolecular complex of PLL, heparin, basic fibroblast growth factor, and fibronectin) for promoting tissue healing and enhancing angiogenesis and axonal regeneration, without increasing inflammation, in the injured rat spinal cord. 50 Further studies at longer implantation times (chronic state) will be necessary to demonstrate the ability of rGO microfibers to enhancing neural repair in the injured spinal cord and to identifying the origin of the cells chronically invading the microfibers. Specifically, current studies in our laboratory are focused on the implementation of the implantation design by modifying the composition and degradation rate of the supportive hydrogel and the functionalization of the microfibers to better favor guidance effects.…”

Section: Results and Discussionmentioning

confidence: 99%

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

et al. 2017

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Neural tissue engineering approaches show increasing promise for the treatment of neural diseases including spinal cord injury, for which an efficient therapy is still missing. Encouraged by both positive findings on the interaction of carbon nanomaterials such as graphene with neural components and the necessity of more efficient guidance structures for neural repair, we herein study the potential of reduced graphene oxide (rGO) microfibers as substrates for neural growth in the injured central neural tissue. Compact, bendable, and conductive fibers are obtained. When coated with neural adhesive molecules (poly-l-lysine and N-cadherin), these microfibers behave as supportive substrates of highly interconnected cultures composed of neurons and glial cells for up to 21 days. Synaptic contacts close to rGO are identified. Interestingly, the colonization by meningeal fibroblasts is dramatically hindered by N-cadherin coating. Finally, in vivo studies reveal the feasible implantation of these rGO microfibers as a guidance platform in the injured rat spinal cord, without evident signs of subacute local toxicity. These positive findings boost further investigation at longer implantation times to prove the utility of these substrates as components of advanced therapies for enhancing repair in the damaged central neural tissue including the injured spinal cord.

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“…SCI causes motor and sensory neuronal deficits induced by the crush of nerve fibers . The restoration and regeneration of axons are still under the way .…”

Section: Discussionmentioning

confidence: 99%

3D printing collagen/chitosan scaffold ameliorated axon regeneration and neurological recovery after spinal cord injury

Sun

Yang

Zhu

et al. 2019

J Biomedical Materials Res

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Spinal cord injury (SCI) is a disaster that can cause severe motor, sensory, and functional disorders. Implanting biomaterials have been regarded as hopeful strategies to restore neurological function. However, no optimized scaffold has been available. In this study, a novel 3D printing technology was used to fabricate the scaffold with designed structure. The composite biomaterials of collagen and chitosan were also adopted to balance both compatibility and strength. Female Sprague–Dawley rats were subjected to a T8 complete‐transection SCI model. Scaffolds of C/C (collagen/chitosan scaffold with freeze‐drying technology) or 3D‐C/C (collagen/chitosan scaffold with 3D printing technology) were implanted into the lesion. Compared with SCI or C/C group, 3D‐C/C implants significantly promoted locomotor function with the elevation in Basso–Beattie–Bresnahan (BBB) score and angle of inclined plane. Decreased latency and increased amplitude were observed both in motor‐evoked potential and somatosensory‐evoked potential in 3D‐C/C group compared with SCI or C/C group, which further demonstrated the improvement of neurological recovery. Fiber tracking of diffusion tensor imaging (DTI) showed the most fibers traversing the lesion in 3D‐C/C group. Meanwhile, we observed that the correlations between the locomotor (BBB score or angle of inclined plane) and the DTI parameters (fractional anisotropy values) were positive. Although C/C implants markedly enhanced biotin dextran amine (BDA)‐positive neural profiles compared with SCI group, rats implanted with 3D‐C/C scaffold displayed the largest degree of BDA profiles regeneration. Collectively, our 3D‐C/C scaffolds demonstrated significant therapeutic effects on rat complete‐transected spinal cord model, which provides a promising and innovative therapeutic approach for SCI. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1898–1908, 2019.

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Biofunctionalized PEDOT-coated microfibers for the treatment of spinal cord injury

Cited by 48 publications

References 73 publications

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4‐Ethylenedioxythiophene):P‐Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

3D printing collagen/chitosan scaffold ameliorated axon regeneration and neurological recovery after spinal cord injury

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