Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance

Abstract

Background Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Methods The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. Results Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-β1 , Fak, and Src . These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype ( Runx2 , Alp , Bsp ). Conclusion Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field.

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

“…Moreover, cells grown on chondroitin sulfate-based samples exhibited noticeably higher ALP activity. In another study, albumin coating on Ti material contributed to increased osteoblast viability [76]. Whereas 3D-polylactic acid (PLA) scaffold coated with gelatin and mucic acid supported cell adhesion, promoted osteogenic differentiation of murine embryonic fibroblast cell line (C3H10T1/2 cell line), and ECM mineralization, as shown by Ashwin et al [77].…”

Surface Functionalisation of Dental Implants with a Composite Coating of Alendronate and Hydrolysed Collagen: DFT and EIS Studies

“…The IR spectra of the alendronate and the implant/alendronate samples ( Figure 2 a) exhibit a characteristic P−O and P=O region (~1200–900 cm −1 ), and the phosphate band at 1520 (1516) cm −1 characteristic for the alendronate [ 37 , 38 ]. The bands are slightly altered by the interaction between alendronate and implant’s surface.…”

Multifunctional Photocatalytic Filter Paper Based on Ultralong Nanowires of the Calcium-Alendronate Complex for High-Performance Water Purification

“…Figure g shows the C 1s fine XPS pattern of the sample synthesized using a Ca/P molar ratio of 1:4.0, which can be divided into the main chain C–C peak at 284.8 eV, C–NH 2 at 285.6 eV, and C–H at 288.0 eV. Figure h shows the O 1s fine XPS pattern of the sample synthesized using a Ca/P molar ratio of 1:4.0, which can be divided into peaks of P–O– (530.5 eV), PO (532.4 eV), and C–O–H (535.3 eV). , Figure i shows the XPS survey spectrum of the sample synthesized using a Ca/P molar ratio of 1:2.5; the analysis results are similar to those of Figure d; and the atomic contents of C, N, O, P, and Ca are approximately 30.37, 3.61, 26.27, 9.05, and 30.71%, respectively.…”