Biofunctionalization of silica microspheres for protein separation

Li, Binjie; Zou, Xueyan; Zhao, Yanbao; Sun, Lei; Li, Shulian

doi:10.1016/j.msec.2013.02.030

Cited by 20 publications

(11 citation statements)

References 23 publications

(22 reference statements)

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“…In the FT-IR spectrum, the peak at 3436 cm −1 is attributed to the OH stretching vibration of hollow silica spheres. The peaks around 1129, 801, and 473 cm −1 are due to Si O Si stretching vibration [27,28]. The intense peak at 2550 cm −1 corresponds to S H stretching vibration.…”

Section: Bet Analysismentioning

confidence: 97%

Functionalized hollow silica nanospheres for His-tagged protein purification

Yin¹,

Wei²,

Zou³

et al. 2015

Sensors and Actuators B: Chemical

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Section: Bet Analysismentioning

confidence: 97%

Functionalized hollow silica nanospheres for His-tagged protein purification

Yin¹,

Wei²,

Zou³

et al. 2015

Sensors and Actuators B: Chemical

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“…Fluorescent microspheres/nanoparticles have been widely used for biosensing and metal ion detection [34][35][36][37]. The entrapped microspheres in a macroporous scaffold could provide a free functional surface with enhanced mass transport and easy removal [3,9,10,38].…”

Section: Synthesis Of Fluorescent Spheres In Emulsion-templated Scaffoldmentioning

confidence: 99%

Silica Microspheres-in-Pores Composite Monoliths with Fluorescence and Potential for Water Remediation

2021

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Water pollution is a severe worldwide issue. Constructing advanced porous composite materials has been an efficient route to water remediation via adsorption. In this study, a unique microspheres-in-pores monolithic structure was fabricated. An emulsion-templated polymer monolith was first prepared and silica microspheres were subsequently formed in the porous polymer. A silica precursor was modified with a fluorescent dye and co-condensed with other precursors to fabricate porous composites with fluorescent properties, which were enhanced by the presence of Ag nanoparticles in the polymer matrix. This unique material showed good promise in water remediation by removing organic dyes and heavy metal ions from wastewater via a flowing filter or monolithic column separation.

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“…5) [64]. These functionalized surfaces can be further modified with antibodies and proteins [64][65][66][67]. Hikosaka et al [64] developed an Immunoglobulin G (IgG) purification affinity material based on this strategy, in which four mesoporous silica materials with two morphologies (spherical (MCM-41s and MCM-48s) and polyhedral (MCM-41p and MCM-48p)) were used.…”

Section: Silica (Nano)particlesmentioning

confidence: 99%

Recovery and Purification of (Bio)Pharmaceuticals Using (Nano)Materials

Tavares¹,

Neves²,

Trindade³

et al. 2020

Recent Advances in Analytical Techniques: Volume 4

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Biopharmaceuticals main classes comprise recombinant proteins, antibodies, and nucleic-acid-derived products, while synthetic pharmaceuticals include a wide variety of organic compounds. The purification of (bio)pharmaceuticals, as part of downstream processing, is mainly carried out by chromatographic processes, which are responsible for the therapeutics high cost. Therefore, there is a crucial need on the development of novel and more efficient separation/purification processes or on the improvement of the current chromatographic-based ones, aiming at producing pharmaceuticals of high quality and at a lower cost. Amongst alternative techniques, it has been demonstrated that inorganic or organic (nano)particles, used in solid-phase extraction approaches, may be promising for the purification of pharmaceuticals. This chapter provides an overview on solid-liquid separation/purification processes used to obtain high purity and high quality pharmaceuticals. The main purification processes are described and summarized. The areas where there has been a sustainable progress, combined with improved therapeutic characteristics, are highlighted. Materials based on silica (nano)particles, carbon-based (nano)particles (carbon nanotubes, graphene and activated carbon) and magnetic (nano)particles are overviewed. Based on the reported results, nanotechnology may play a key role in future pharmaceutical developments and manufacturing, where the design of suitable functionalized (nano)particles is a crucial factor to enhance the selectivity and to obtain high purification and recovery yields of (bio)pharmaceuticals.

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Biofunctionalization of silica microspheres for protein separation

Cited by 20 publications

References 23 publications

Functionalized hollow silica nanospheres for His-tagged protein purification

Functionalized hollow silica nanospheres for His-tagged protein purification

Silica Microspheres-in-Pores Composite Monoliths with Fluorescence and Potential for Water Remediation

Recovery and Purification of (Bio)Pharmaceuticals Using (Nano)Materials

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