Biofunctionalization of Selenium Nanoparticle with Dictyophora Indusiata Polysaccharide and Its Antiproliferative Activity through Death-Receptor and Mitochondria-Mediated Apoptotic Pathways

Abstract

Bio-functionalized nanoparticles with semiconducting/metallic core encapsulated in a bio- or bio-derived materials are promising for applications in biology and especially in cancer diagnostic and healing. In this report, we report a facile, single-step, first-time synthesis and in-situ functionalization strategy for the preparation of monodispersed selenium nanoparticles (SeNPs) functionalized using a novel polysaccharide (DP1) extracted from Dictyophora indusiata (a fungus). The DP1 functionalized SeNPs (DP1-SeNPs), where DP1 is attached to the surface via Se-O bond as well as physic-sorption had, an average diameter of 89 nm, and were highly uniform, extremely stable compared to bare SeNPs. Detailed investigation of the biological properties of DP1-SeNP illustrated that they exhibit unprecedented, enhanced, and selective antiproliferative activity through inducing cell apoptosis confirmed by nuclear condensation, DNA cleavage, and accumulation of S phase cell arrest. The mechanism of the induced apoptosis was found to be a combination of the activation of caspases 3, 8, and 9, the Fas-associated death domain protein (FADD), reactive oxygen species (ROS) overproduction, as well as mitochondrial dysfunction. It is envisioned that the reported DP1-SeNPs will offer a new phase space for high-efficiency anticancer treatment with little side effect.

Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

“…The microplate was oscillated for 10 min. Measure the value of absorbance by enzymelinked immunosorbent assay at the wavelength of 490 nm [37]. Calculate the relative degree of proliferation of the cells called relative growth rate (RGR) according to the formula.…”

Scaffold of Selenium Nanovectors and Honey Phytochemicals for Inhibition of Pseudomonas aeruginosa Quorum Sensing and Biofilm Formation

“…Among these nanomaterials, selenium nanoparticles (SeNPs) have garnered a great deal of attention as efficient drug carriers (Liu et al, 2012; Yu et al, 2014; Xia et al, 2015). As a special Se species, the SeNPs are also recognized because of their excellent antioxidant property and disease preventive effects (Torres et al, 2012; Liao et al, 2015). Abundant evidences also support the better biocompatibility, bio-efficacy and lower toxicity of the SeNPs by comparing with inorganic and organic seleno-compounds (Husen and Siddiqi, 2014).…”

Nanoparticles Carrying Biological Molecules: Recent Advances and Applications

“…For example, the nature-evolved multiple binding sites of biomolecules in addition with its catalytic properties could facilitate the development of multifunctional nanoparticles (Katz E. and Willner I., 2004). Recently, substantial research efforts were directed towards developing and extending the applications of biomolecules by integrating the biomolecules with biological nanoparticles such as polysaccharides and lipids (Liao W. et al, 2016;Rassu G. et al, 2015) and other types of nanoparticles including metals (Chinen A.B. et al, 2015;Politi J. et al, 2015), metal oxides (Cao Y. et al, 2016;Shahrestani H. et al, 2016) and polymers (Cavalli R. et al, 2011;Lin T.-T. et al, 2016) through various conjugation strategies ( Table 2).…”

“…These challenges can be potentially solved by conjugation of biomolecules with biological nanoparticle through strategic functionalisation. Liao W. et al (2016) reported that selenium nanoparticles functionalised with a Dictyophora indusiata polysaccharide formed monodispersed nanoparticles with high stability attributed to the high electrostatic repulsion between hydrophilic moieties on the surface of the polysaccharide in comparison with the bare selenium. Moreover, the nanoparticle conjugate exhibited enhanced selectivity and antiproliferative activity by inducing cell apoptosis.…”