Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

Abstract

Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light-and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm 2 : each p < 0.05; branching points per mm 2 : each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

“…A vacutainer system and specific sterile plain vacuum tubes with additional silicone within their coating surface were, respectively, used for solid (A-PRF+, Mectron, Carasco, Italy) and liquid PRF (iPRF, Mectron, Carasco, Italy). PRF was directly manufactured with a fixed angle rotor with a radius of 110 mm at 1200 rpm and a relative centrifugal force of 177 g for 8 min (Duo centrifuge, Mectron, Carasco, Italy) following the manufacturer’s instructions [ 7 ].…”

“…In contrast, autologous platelet concentrates such as platelet-rich fibrin (PRF) that are now broadly used in dental and craniomaxillofacial regenerative medicine may potentially overcome this limitation [ 5 ]. Thus far, the pro-angiogenic effect of the PRF has been demonstrated to mainly impact soft tissue regeneration procedures [ 6 , 7 ], but the complex interplay of different cytokines and growth factors leads to an increased proliferation and differentiation of different cell lines, inter alia, osteoblasts [ 8 , 9 ]. In detail, vascular endothelial growth factor (VEGF), tissue growth factor ß (TGFß), and platelet-derived growth factor (PDGF) have been discussed to trigger vasoformative responses [ 10 ].…”

“…Recently, our working group showed a positive effect of PRF in combination with allogeneic and xenogeneic BSMs that enhanced osteoblast activity compared to a native BSM in vitro at later time points [ 7 ]. Thus far, no comparative study has analyzed the early angiogenetic interactions of PRF with different BSMs in vitro nor translated these results to in vivo experiments.…”

A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

“…These membranes mostly consist of porcine or bovine collagen and are characterized by a good barrier function [3,6]. As a biomaterial, collagen has a number of properties including low immunogenicity, fast vascularization, promotion of wound healing and even bone regeneration [7][8][9][10][11]. It was shown that the material allows sufficient diffusion of nutrients for cellular proliferation and differentiation [12].…”

A Collagen Membrane Influences Bone Turnover Marker in Vivo After Bone Augmentation With Xenogenic Bone

“…These membranes mostly consist of porcine or bovine collagen and are characterized by a good barrier function [3,6]. As a biomaterial, collagen has a number of properties including low immunogenicity, fast vascularization, promotion of wound healing and even bone regeneration [7][8][9][10][11]. It was shown that the material allows su cient diffusion of nutrients for cellular proliferation and differentiation [12].…”