2020
DOI: 10.20944/preprints202011.0195.v1
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Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk in vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction

Abstract: Protein hydrolysates show great promise as bioactive food and feed ingredient and for valorization of side-streams from e.g. the fish processing industry. This study characterizes bulk emulsifying, foaming, and in vitro antioxidative properties of hydrolysates derived from cod frame by application of Alcalase and Neutrase, individually and sequentially as well as the influence of heat-treatment prior to hydrolysis. We present a novel approach that utilizes proteomics data for calculation of weighted mean pepti… Show more

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Cited by 7 publications
(6 citation statements)
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“…Degree of hydrolysis (DH) was determined based on α-amino nitrogen content as previously described (Jafarpour, Gregersen, et al, 2020) with minor modifications, adjusting for the α-amino nitrogen content of the untreated substrate. Briefly, free α-amino content of PPHs was determined using the PFAN-25 free amino nitrogen assay kit (PractiChrom, USA) measured using A 530 on a Picoexplorer (USHIO INC, USA), according to manufacturer guidelines, using glycine as reference for standard curve generation.…”
Section: Methodsmentioning
confidence: 99%
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“…Degree of hydrolysis (DH) was determined based on α-amino nitrogen content as previously described (Jafarpour, Gregersen, et al, 2020) with minor modifications, adjusting for the α-amino nitrogen content of the untreated substrate. Briefly, free α-amino content of PPHs was determined using the PFAN-25 free amino nitrogen assay kit (PractiChrom, USA) measured using A 530 on a Picoexplorer (USHIO INC, USA), according to manufacturer guidelines, using glycine as reference for standard curve generation.…”
Section: Methodsmentioning
confidence: 99%
“…Emulsifying activity index (EAI) and emulsion stability index (ESI) were determined using the method described by (Pearce & Kinsella, 1978) with slight modifications, previously described (Jafarpour, Gregersen, et al, 2020). Briefly, 15 mL PPH in distilled water (2 mg/mL) was mixed with 5 mL rapeseed oil by an ultraturax homogenizer (IKA, Germany) at speed of 9,500 rpm for 60 s without pH adjustment.…”
Section: Methodsmentioning
confidence: 99%
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