Biofunctional alendronate–Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation

Bigi, Adriana; Boanini, Elisa; Capuccini, Chiara; Fini, Milena; Mihăilescu, I. N.; Sima, Félix; Torricelli, Paola

doi:10.1016/j.biomaterials.2009.07.066

Cited by 66 publications

(43 citation statements)

References 32 publications

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“…38 Unlike the reported controversy regarding the effects of soluble BPs, alendronate loaded either on CaP nanocrystal or CaP coating resulted in increased osteoblast proliferation and higher values of differentiation parameters. 17,47 In vivo studies also indicated that implantation of BP-loaded biomaterials resulted in a significant increase in relative bone content, and an improvement of its micro-architecture and mechanical fixation. 48,49 FIG.…”

Section: Osteoblast Attachment Proliferation Differentiation and Amentioning

confidence: 99%

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AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Neoh

Shi

et al. 2013

Tissue Engineering Part A

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Fibrous encapsulation can impair implant osseointegration and cause implant failure but currently there are limited strategies to address this problem. Since bisphosphonates (BPs), a class of drugs widely used to treat bone diseases, was recently found to induce fibroblast apoptosis, we hypothesize that by loading BPs on titanium (Ti) implant surface, fibrous encapsulation may be inhibited with simultaneous enhancement of implant osseointegration. This strategy of local administration can also be expected to minimize the adverse side effects of BPs, which are associated with intravenous injections. To verify this hypothesis, alendronate was loaded on Ti surface via a hydroxyapatite (CaP) coating, and the effects of the loaded alendronate on fibroblast proliferation and apoptosis, and osteoblast proliferation, alkaline phosphatase (ALP) activity, and apoptosis were investigated in vitro. With a surface density of loaded alendronate 0.046 mg/cm 2 or higher, fibroblast proliferation was suppressed due to increased apoptosis, while osteoblast proliferation and ALP activity increased with minimal apoptosis. In a coculture of fibroblasts and osteoblasts in a 1:1 ratio, *60% of the cells on these alendronate-loaded substrates were osteoblasts 1 day after cell seeding. The percentage of osteoblasts increased to about 75% 4 days after cell seeding. These results suggest that fibroblasts and osteoblasts respond differently toward the alendronate-modified substrates, and this phenomenon can potentially be capitalized to reduce fibrous encapsulation.

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Section: Osteoblast Attachment Proliferation Differentiation and Amentioning

confidence: 99%

“…[16][17][18] In addition to the effects of BPs on osteoclasts and osteoblasts, recent studies indicated that BPs, especially the highly potent nitrogen-containing BPs, can inhibit fibroblast proliferation and increase their apoptosis. [19][20][21][22] Thus, treatment with BPs may help to reduce fibrous encapsulation and enhance implant osseointegration.…”

mentioning

confidence: 99%

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Neoh

Shi

et al. 2013

Tissue Engineering Part A

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“…The typical surface morphology of thin films deposited by MAPLE technique is characterized by an aggregated structure, consisting of micrometer-sized particles [17,18,22,39,119]. It is worth noting that a larger specific surface area was proven to induce an enhanced bioactivity, able to promote osteoblast differentiation, as reported in case of hybrid organic-inorganic thin films deposited by MAPLE [120,121].…”

Section: Hybrid Dextran-iron Oxide Thin Filmsmentioning

confidence: 78%

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Axente¹,

Sima²,

Ristoscu³

et al. 2016

Recent Advances in Biopolymers

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This chapter provides an overview of recent advances in the field of laser-based synthesis of biopolymer thin films for biomedical applications. The introduction addresses the importance of biopolymer thin films with respect to several applications like tissue engineering, cell instructive environments, and drug delivery systems. The next section is devoted to applications of the fabrication of organic and hybrid organic-inorganic coatings. Matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE are introduced and compared with other conventional methods of thin films assembling on solid substrates. Advantages and limitations of the methods are pointed out by focusing on the delicate transfer of bio-macromolecules, preservation of properties and on the prospect of combinatorial libraries' synthesis in a single-step process. The following section provides a brief description of fundamental processes involved in the molecular transfer of delicate materials by MAPLE. Then, the chapter focuses on the laser synthesis of two polysaccharide thin films, namely Dextran doped with iron oxide nanoparticles and Levan, followed by an overview on the MAPLE synthesis of other biopolymers. The chapter ends with summary and perspectives of this fast-expanding research field, and a rich bibliographic database.

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“…The data demonstrated MAPLE fabrication can be applied to synthesize coatings that synergistically couple the bioactivity of HA with the local availability of alendronate to more safely improve bone metabolism for the patients with osteoporosis or fibrous dysplasia. 77 Ideally, biomaterials to be deposited by MAPLE should have minimal laser interaction. Doraiswamy et al used triazine polymer as an intermediate absorbing layer and transferred viable B35 neuroblasts using MAPLE.…”

Section: Biomaterials Deposition Using Matrix Assisted Pulsed Lasementioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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Biofunctional alendronate–Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation

Cited by 66 publications

References 32 publications

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Laser-assisted biofabrication in tissue engineering and regenerative medicine

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