Biofuels: Bioethanol, Biodiesel, Biogas, Biohydrogen from Plants and Microalgae

Gharabaghi, Mahdi; Amrei, Hossein Delavai; Zenooz, Alireza Moosavi; Guzullo, Jafar Shahrivar; Ashtiani, Farzin Zokaee

doi:10.1007/978-3-319-11906-9_6

Cited by 11 publications

(5 citation statements)

References 54 publications

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“…Current oil production derives mainly from oleaginous crops and non-renewable fossil reserves, whose utilization causes a negative environmental impact (Alptekin et al, 2014; Moody et al, 2014; Majidian et al, 2018). It has been shown that the use of biodiesel over fossil fuels decreases greenhouse gas emissions and crop cultivation for fuel production creates a direct competition with food and feed production (Gharabaghi et al, 2015; Hanaki and Portugal-Pereira, 2018). Additionally, agricultural crop cultivation for oil production requires large amounts of arable lands and fresh-water.…”

Section: Introductionmentioning

confidence: 99%

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans

et al. 2019

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Microalgal lipids are promising feedstocks for food and biofuels. Since lipid production by microalgae is not yet economically feasible, genetic engineering is becoming a promising strategy to achieve higher lipid accumulation and productivities. Enzymes involved in the Kennedy pathway such as glycerol-3-phosphate acyltransferase (GPAT), lysophosphatidic acid acyltransferase (LPAT), and diacylglycerol acyltransferase (DGAT) catalyze key steps in the formation of triacylglycerol, which is the main constituent of lipids in N. oleoabundans. The overexpression of these enzymes in the targeted strain has a great potential to further increase their triacylglycerol content. We overexpressed single and multiple encoding genes for LPAT, GPAT, and DGAT from Acutodesmus obliquus in N. oleoabundans. Strains overexpressing single genes produced up to 52% and 45% g · gDW-1, which corresponds to 1.3- and 1.4-fold increase in total fatty acids and triacylglycerols, respectively. The orchestrated expression of the three genes resulted in 49% and 39% g · gDW-1, which is 1.2-folds increase in total fatty acids and triacylglycerols. Single expression of LPAT, GPAT, and DGAT genes resulted in higher lipid productivities during starvation without a significant effect on growth and photosynthetic activity during replete conditions. On the other hand, the simultaneous expression of LPAT, GPAT, and DGAT genes resulted in 52% lower growth rate, 14% lower photosynthetic activity and 4-folds increase in cell diameter. Moreover, the multigene expressing line showed a decrease in carbohydrates and protein content and an increase in pigments during nitrogen starved condition. The single and multiple expression of heterologous genes LPAT, GPAT, and DGAT showed to significantly enhanced the lipid accumulation in N. oleoabundans. Single gene expression resulted in higher lipid production and productivities without having a significant impact in the physiological status of the strains. This approach shows the potential for the generation of microalgal strains with higher economical potential for the production of lipids.

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Section: Introductionmentioning

confidence: 99%

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans

et al. 2019

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“…According to some research, more carbon dioxide will be produced due to growing manufacturing industry, forms of transport and human activity (Belbute and Pereira, 2015;Monastersky). Despite the fact that the utilization of fossil fuels (oil and coal) is the decisive factor in global warming and climate change, the widespread use of an alternative renewable source of energies can play an important role in reducing environmental pollution and preventing using non-renewable resources, which are quite limited in the world (Gharabaghi et al, 2015;Amin, 2009;McKendry, 2002;Sawayama et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

CARBON DIOXIDE BIOFIXATION BY Chlorella sp. IN A BUBBLE COLUMN REACTOR AT DIFFERENT FLOW RATES AND CO2 CONCENTRATIONS

Pourjamshidian

Abolghasemi

Esmaili

et al. 2019

Braz. J. Chem. Eng.

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CO 2 biofixation of the microalgae Chlorella sp. for different CO 2 concentrations and gas flow rates in a bubble column reactor has been investigated in this study. Microalgae were cultivated under different CO 2 concentrations (at 1.75% and 9.45% v/v) and gas flow rates (at 30, 50 and 70 ml/min). The maximum specific growth rate of Chlorella sp. was obtained for the CO 2 concentration of 1.75 % and the gas flow rate of 50 mL/min. The highest biomass productivity rate (at 0.17 g L-1 day-1) was for a sample with 1.75 % CO 2 at a flow rate of 70 ml/min. Moreover, the results have shown that the specific growth rate and CO 2 biofixation have a direct relation with culturing of Chlorella sp. Also, limiting CO 2 supplementation noticeably decreased biomass concentration. Therefore, the results have shown that a high flow rate and low concentration of CO 2 might promote a decrease in CO 2 fixation efficiency by Chlorella sp.

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“…Current oil production derives mainly from oleaginous crops and non-renewable fossil reserves, whose utilization causes a negative environmental impact (Alptekin et al, 2014;Majidian et al, 2018;Moody et al, 2014). It has been shown that the use of biodiesel over fossil fuels decreases greenhouse gas emissions and crop cultivation for fuel production creates a direct competition with food and feed production (Gharabaghi et al, 2015;Hanaki & Portugal-Pereira, 2018). Additionally, agricultural crop cultivation for oil production requires large amounts of arable lands and freshwater.…”

Section: Introductionmentioning

confidence: 99%

Genetic engineering of microalgae for enhanced lipid production

Muñoz

Südfeld

Naduthodi

et al. 2021

Biotechnology Advances

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Genetic transformation of microalgae remains a challenge due to poor intracellular delivery of exogenous molecules. This limitation is caused by the structure and composition of the cell wall and cell membrane of each species.Moreover, successful delivery of proteins or nucleic acids cannot be assessed by determining transformability since their functionality is not always known in the studied microorganism. We propose a quick and effective screening tool for the prediction and optimization of electroporation settings by monitoring cell permeability and viability using Sytox Green and propidium iodide respectively.We determined voltage settings for the microalgae Chlamydomonas reinhardtii, Chlorella vulgaris, Neochloris oleoabundans and Acutodesmus obliquus. To evaluate the predicted settings, we delivered labelled DNA and proteins into the cells. We demonstrated that high transformation efficiencies can be accomplished when predicted values were applied with functional plasmids.Additionally, we increased transformation efficiencies by testing cell concentrations, light intensities and fragment sizes. This method can be used to determine suitable transformation conditions for non-transformed microalgae species and to increase the insight on established transformation protocols.

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Biofuels: Bioethanol, Biodiesel, Biogas, Biohydrogen from Plants and Microalgae

Cited by 11 publications

References 54 publications

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans

CARBON DIOXIDE BIOFIXATION BY Chlorella sp. IN A BUBBLE COLUMN REACTOR AT DIFFERENT FLOW RATES AND CO2 CONCENTRATIONS

Genetic engineering of microalgae for enhanced lipid production

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