Bioflocs protects copper-induced inflammatory response and oxidative stress in Rhynchocypris lagowski Dybowski through inhibiting NF-κB and Nrf2 signaling pathways

Yu, Zhe; Zheng, Ya-Guang; Du, Hong‐Lin; Li, Hong-Jin; Wu, Li‐Fang

doi:10.1016/j.fsi.2020.01.048

Cited by 45 publications

(22 citation statements)

References 51 publications

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“…At the end of the feeding trial, remaining fish were added with anhydrous copper sulphate (CuSO 4 ) mother liquor to carry out acute waterborne copper exposure test. According to our previous study (Yu, Zheng, Du, Li, et al, 2020), the semi-lethal concentration of waterborne copper exposure to R. lagowski for 96 hr was 1.03 mg/L.…”

Section: Challenge Testmentioning

confidence: 83%

“…Amylase (AMS) activity , lipase (LPS) activity (Long, Yang, Li, Guan, & Wu, 2015), protease (PRS) activity (Xu et al, 2013), cellulase (CES) activity ; lysozyme (LZM) activity , complement C3, C4 (Li, Zhu, Tian, Liu, & Wang, 2019) and alkaline phosphatase (AKP) activity ; total superoxide dismutase (T-SOD) activity (Chen, Ren, Wang, Xia, & Li, 2018), total antioxidant capacity (T-AOC) activity (Yu, Li, Zhu, Li, Duan, et al, 2020), catalase (CAT) activity (Yu, Zheng, Du, Li, et al, 2020), ascorbic acid (ASA) activity (Feng et al, 2016), glutathione peroxidase (GSH-PX) activity (Li et al, 2020)…”

Section: Digestion Immunity and Antioxidation Parametersmentioning

confidence: 99%

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Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

Huang

et al. 2020

Aquacult. Nutr.

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This study compared the influences of differential protein levels of feed on growth, copper-induced digestive enzyme activity, immune response, oxidative stress and Nrf2 signalling molecules genes expression of Rhynchocypris lagowski in a bioflocbased system and traditional autotrophic system. Four hundred and fifty R. lagowski were fed four experimental dietary containing graded levels of protein from 25% to 37% (referred as biofloc technology [BFT]25, BFT29, BFT33 and BFT37, respectively) in a biofloc-based system, and 37% crude protein level (control group) were cultured in an autotrophic system in 8 weeks. At the end of the trial, there was an improvement of 50.35%-61.64% in terms of growth performance (weight gain rate); 0.25-0.53 (U/mg prot) amylase; 6.9-8.02 (U/ml) lysozyme (LZM); and 7.98-8.12 (U/ ml) total superoxide dismutase (T-SOD) in R. lagowski cultured in BFT37 groups as compared to control. Obviously, the growth performance, digestive enzymes activity, immune response, antioxidant statue and antioxidant-related genes expression of R. lagowski fed with the same protein level in BFT system were significantly higher than those in the control group (p < .05). Furthermore, the level of T-SOD in liver and kidney and interferon-γ in liver of BFT33 group were also significantly higher than that of control group (p < .05). Moreover, there was no notable difference of LZM, alkaline phosphatase, complement C3, C4, heat shock proteins 70, heat shock proteins 90, insulin-like growth factor I, glutathione peroxidase, catalase, total antioxidant capacity, malondialdehyde, Ascorbic acid in gill, liver, kidney, gut and serum among the BFT29 groups and those in the control (p > .05). Besides, bioflocs assists in regulating the expression of Nrf2 signalling molecules including Nrf2, Keap1, Maf, HO-1, CAT, CuZn-SOD, GPX and GCLC. We summarized that despite the low protein content in feed, BFT system has the potential to promote growth performance, alleviate copper-induced immunosuppression and oxidative stress of juvenile R. lagowski.

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Section: Challenge Testmentioning

confidence: 83%

Section: Digestion Immunity and Antioxidation Parametersmentioning

confidence: 99%

Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

Huang

et al. 2020

Aquacult. Nutr.

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“…Amylase (AMS) activity, lipase (LIS) activity, protease (PRS) activity, lysozyme (LSZ) activity, alkaline phosphatase (AKP) activity, superoxide dismutase (SOD) activity, total antioxidant capacity (T‐AOC), catalase (CAT) activity, malondialdehyde (MDA), ascorbic acid (ASA), glutamic pyruvic transaminase (GPT) activity and glutamic oxaloacetic transaminase (GOT) activity were determined using commercial assay kits (Nanjing Jiancheng Institute) according to the protocol of Li, Li, et al (2019), Li, Liu, et al (2019), Li, Zhu, et al (2019), Yu, Li, Zhu, Li, Duan, et al (2020), Yu, Li, Zhu, Li, and Wu (2020), Yu, Wu, et al (2020), and Yu, Zheng, et al (2020).…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation of microbial floc heightens growth and improves digestive, immune, antioxidant enzymes activity and ammonia resistance in Opsariichthys kaopingensi s

et al. 2020

Aquac Res

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The present study was conducted to evaluate the effects of dietary supplementation of microbial floc on growth, digestive and immune enzymes activity, antioxidant status and ammonia resistance in Opsariichthys kaopingensis. Four hundred fifty healthy O. kaopingensis juveniles (6.21 ± 0.32 g) were randomly assigned to 15 tanks. Juvenile fish were fed five experimental diets containing microbial floc 0 (B0 control), 4% (B4), 8% (B8), 12% (B12) and 16% (B16), respectively, for 30 days. Results showed that B12 could significantly enhance weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER), reduce feed conversion rate (FCR) (p < .05), compared to the control. The activity of protease, lipase and amylase in hepatopancreas and gut of B12 group were higher than that of the control (p < .05). Also, significant increases in lysozyme (LSZ), glutamic pyruvic transaminase (GPT) and alkaline phosphatase (AKP) activities of O. kaopingensis were found at B12 group (p < .05). Comparing the antioxidant capacity, the activities of superoxide dismutase (SOD), total antioxidant capacity (T‐AOC), catalase (CAT) and ascorbic acid (ASA) of juveniles in B12 group were significant highly (p < .05) than control, and the content of malondialdehyde (MDA) was much lower than control in gill, kidney, hepatopancreas, intestine and serum under this experiment condition. After ammonia challenge, survival rate in all dietary supplementation of microbial floc treatment groups was significantly increased (p < .05). Overall, these findings suggest that dietary supplementation of microbial floc enhanced growth, digestion, antioxidant and immune response, and increased ammonia stress resistance in O. kaopingensis. The suitable supplemental level of approximately 12% microbial floc was recommended in the present study.

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“… 17 , 18 On the other hand, unnecessarily high additions of Cu in fish can cause toxicity in the tissue structure, leading to changes in osmotic response and acid–base regulatory system. 19 , 20 In an aquaculture system, dietary intake is the major way of Cu acquisition for fish. 21 , 22 Accordingly, it is necessary for aquaculture producers to give priority to Cu sources with higher bioavailability in the intensification of the breeding system.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Efficacy of the N-carbamoyl-methionine Copper on the Growth Performance, Tissue Mineralization, Immunity, and Enzymatic Antioxidant Capacity of Nile tilapia (Oreochromis niloticus)

et al. 2020

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Immunogenic, methionine copper-induced response had proven to be precedent in providing resistance against certain diseases in fish. This study allocates the fitness strategy for Oreochromis niloticus by introducing and incorporating the well-designed, stabilized, and biocompatible N -carbamoyl-methionine copper (NCM-Cu) as a Cu potent source in diet that enhances the bioavailability and fitness. The synchronized NCM-Cu complex was characterized by directing ultraviolet and visible spectrophotometry (UV–vis), Fourier-transform infrared (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA), and single-crystal X-ray diffraction. Results revealed blue columnar crystalline, NCM-Cu complex with an empirical formula as C 12 H 30 CuN 4 O 10 S 2 . Anonymously, the overall growth performance of the fish remained unaltered with NCM-Cu adjunct feed. NCM-Cu significantly raised the Cu accumulation in the fish muscles, liver, gill, and intestine in contrast to the basic Cu-rich feed. The serum antioxidant enzyme activity elevated up to (ceruloplasmin: 19.38 U/L) and the lowest liver malondialdehyde (MDA) content (8.81 nmol/mg prot.) and triglyceride content (0.39 nmol/g prot.) were observed in the NCM-Cu group as compared to the basic Cu and CuSO 4 groups, suggesting that NCM-Cu promoted antioxidative responses and alleviated lipid peroxidation of O. niloticus . Overweening, the synthesized complex, NCM-Cu significantly regulated the expression levels of lysozyme, immunoglobulin M, complement 4, and complement 3 up to 10.93 U/mL, 0.72, 0.77, and 1.18 mg/mL in serum, respectively. Thus, such endorsed results reveal the preeminence of NCM-Cu-supplemented diet for the fitness in O. niloticus .

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Bioflocs protects copper-induced inflammatory response and oxidative stress in Rhynchocypris lagowski Dybowski through inhibiting NF-κB and Nrf2 signaling pathways

Cited by 45 publications

References 51 publications

Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

Dietary supplementation of microbial floc heightens growth and improves digestive, immune, antioxidant enzymes activity and ammonia resistance in Opsariichthys kaopingensi s

Synthesis and Efficacy of the N-carbamoyl-methionine Copper on the Growth Performance, Tissue Mineralization, Immunity, and Enzymatic Antioxidant Capacity of Nile tilapia (Oreochromis niloticus)

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