Bioflocculants’ production from a cellulase-free xylanase-producing Pseudomonas boreopolis G22 by degrading biomass and its application in cost-effective harvest of microalgae

Guo, Haipeng; Hong, Chuntao; Zhang, Cheng; Zheng, Bingsong; Jiang, Dean; Qin, Wensheng

doi:10.1016/j.biortech.2018.01.082

Cited by 38 publications

(22 citation statements)

References 45 publications

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“…were evaluated. Similarly, Guo et al (2018) investigated bioflocculant production of Pseudomonas boreopolis G22 under different pHs (5 -10). In the present study, sucrose was the most favorable carbon source for bioflocculant production by B. megaterium LBPMA-APFSG3Isox, and B. toyonensis LBPMA-ACOPR1.Isox, respectively, with 41% and 39% bioflocculating activity after 24 h. For B. pumilus LBPMA-BLD07 and B. thuringiensis LBPMA-EFIII, maltose was the best source of carbon to stimulate this bioflocculant activity (13% and 25%, respectively) ( Figures 2 and 3).…”

Section: Optimization Of Culture Medium For Bioflocculant Productionmentioning

confidence: 99%

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Gouveia

Silva

Santos

et al. 2019

Braz. J. Chem. Eng.

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Biological flocculants are advantageous due to their biodegradability and safety to living beings. In this work, four bacterial strains, isolated from sugarcane cropped soil (Bacillus megaterium LBPMA-APFSG3Isox and B. toyonensis LBPMA-ACOPR1.Isox) and sludge of an agroindustrial effluent (B. pumilus LBPMA-BLD07 and B. thuringiensis LBPMA-EFIII), were studied. It was found that all of them secreted bioflocculants, at 37 ± 1 °C, with no pH changes over time and the flocculant activity increased during the time course of incubation. These results stimulated the optimization of the culture conditions to improve flocculation rates, such as the pH, nitrogen sources and carbon. For B. toyonensis LBPMA-ACOPR1.Isox and B. thuringiensis LBPMA-EFIII, the best pH for the bioflocculant production was 5.0. Sucrose and maltose were the best sources of carbon, while urea was the preferred source of nitrogen for two of the tested isolates (B. pumilus LBPMA-BLD07 and B. toyonensis LBPMA-ACOPR1.Isox), followed by (NH 4)2SO 4 (B. megaterium LBPMA-APFSG3Isox) and peptone (B. thuringiensis LBPMA-EFIII). The FTIR-ATR spectra of each extracted material responsible for the flocculant activities of the strains displayed carboxyl, hydroxyl and methoxy functional groups characteristic of polysaccharides.

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Section: Optimization Of Culture Medium For Bioflocculant Productionmentioning

confidence: 99%

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Gouveia

Silva

Santos

et al. 2019

Braz. J. Chem. Eng.

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“…The strain G22, which produces a cellulase-free xylanase, was isolated from paper mill sludge in our previous study [ 30 ]. To evaluate its ability to degrade hemicellulose in a wheat straw pretreatment, the xylanase activity of G22 was assayed.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, a cellulase-free xylanase-producing bacterial strain, Pseudomonas boreopolis G22, was obtained from paper mill sludge and used to produce bioflocculants though directly using untreated biomass as the sole carbon source. Of our interest, the cellulose contents of certain biomasses were significantly increased along with the removal of hemicellulose after 6 days of incubation by G22 [ 30 ]. In this study, we aim to confirm whether a G22 pretreatment could increase the digestibility of wheat straw by incubating wheat straw biomass with G22 for different days, followed by evaluating its digestibility using enzymatic hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

Improving enzymatic digestibility of wheat straw pretreated by a cellulase-free xylanase-secreting Pseudomonas boreopolis G22 with simultaneous production of bioflocculants

Guo¹,

Hong²,

Zheng³

et al. 2018

Biotechnol Biofuels

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BackgroundXylan removal by bacterial pretreatments has been confirmed to increase the digestibility of biomass. Here, an effective xylan removal technique has been developed to enhance the digestibility of wheat straw and simultaneously produce bioflocculants by a cellulase-free xylanase-secreting strain, Pseudomonas boreopolis G22.ResultsThe results indicated that P. boreopolis G22 is an alkaliphilic strain which can secrete abundant amounts of xylanase. This xylanase had activity levels of 2.67–1.75 U mL−1 after an incubation period of 5–25 days. The xylanase showed peak activity levels at pH 8.6, and retained more than 85% relative activity in the pH range of 7.2–9.8. After 15 days of cultivation, the hemicellulose contents of the wheat straw were significantly decreased by 32.5%, while its cellulose contents were increased by 27.3%, compared to that of the control. The maximum reducing sugars released from the 15-day-pretreated wheat straw were 1.8-fold higher than that of the untreated wheat straw, under optimal enzymatic hydrolysis conditions. In addition, a maximum bioflocculant yield of 2.08 g L−1 was extracted from the fermentation broth after 15 days of incubation. The aforementioned bioflocculants could be used to efficiently decolorize a dye solution.ConclusionsThe results indicate that the cellulase-free xylanase-secreting P. boreopolis G22 may be a potential strain for wheat straw pretreatments. The strain G22 does not only enhance the enzymatic digestibility of wheat straw, but also simultaneously produces a number of bioflocculants that can be used for various industrial applications.

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“…While using untreated wood dust, the strain produced a 3.982×10 5 Da polysaccharidic polymer (63.3%). This polymer flocculates kaolin suspension (FA: 97.1% obtained at a concentration of 3.5 mg l −1 ) and microalgal biomass (FA: 95.7% obtained at 80 mg l −1 ) (Guo et al 2018a). Likewise, Pseudomonas veronii L918, with its enzymatic system produced in the presence of peanut hull hydrolysate a bioflloculant useful for ash-flushing wastewater treatment (Liu et al 2016).…”

Section: Agro-industrial Materials For Microbial Bioflocculant Producmentioning

confidence: 99%

Agro-industrial waste materials and wastewater as growth media for microbial bioflocculants production: a review

Siddeeg

Tahoon

Rebah

2019

Mater. Res. Express

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Various microbial strains (bacteria, fungi, and microalgae) produced polymers variable in composition (protein, cellulose, polysaccharide, etc) with interesting flocculation properties such as the ability to remove large spectrum of pollutants (organic and inorganic materials, etc) from wastewater and the stability over a wide range of temperature, pHs and salt concentrations. These bioflocculants have been characterized and successfully tested in wastewater treatment and sludge dewatering. The production of microbial bioflocculants involves the culture step of the bioflocculant-producing microorganism in an appropriate medium, followed by polymer extraction. The production processing is mostly controlled by the microbial growth medium cost. Agro-industrial wastes including agricultural by-products (rice hull, rice stover potato by-products, peanut hull, corn cob, wheat bran, etc), sugar processing wastes and fermentation liquors contain nutrients such as nitrogen and carbon, which can sustain the microbial growth and bioflocculant production. Recently, the potential use of wastewater and sludge as growth media for various bioflocculant-producing microorganisms has been demonstrated. Interestingly, waste pre-treatments may be essential to enhance the microbial growth and the bioflocculant production. Bioflocculant properties (polymer yield, polymer composition, flocculating activity, etc) are controlled by the growth conditions. Moreover, the produced materials showed acceptable results for wastewater treatment and sludge dewatering. This new strategy reported in this review can decrease to some extent the environmental problems related to the disposal of agro-industrial wastes and wastewater sludges. At the same time, this could reduce the cost of microbial bioflocculant production.

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Bioflocculants’ production from a cellulase-free xylanase-producing Pseudomonas boreopolis G22 by degrading biomass and its application in cost-effective harvest of microalgae

Cited by 38 publications

References 45 publications

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Improving enzymatic digestibility of wheat straw pretreated by a cellulase-free xylanase-secreting Pseudomonas boreopolis G22 with simultaneous production of bioflocculants

Agro-industrial waste materials and wastewater as growth media for microbial bioflocculants production: a review

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