Biofilms possibly harbor occult SARS-CoV-2 may explain lung cavity, re-positive and long-term positive results

He, Daqian; Fu, Chaojiang; Ning, Mingjie; Hu, Xiaoxin; Li, Shanshan; Chen, Ying

doi:10.3389/fcimb.2022.971933

Cited by 3 publications

(3 citation statements)

References 83 publications

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“…Studies have been performed to establish the hypothesis that SARS-CoV-2 may co-exist in biofilms with other micro-organisms, which needs more evidence and clinical observation [ 17 ]. Most biofilms are surface-associated with biotic (e.g.…”

Section: Discussionmentioning

confidence: 99%

Post-COVID-19 cavitary lung lesion due to Aspergillus flavus and Enterobacter cloacae in a patient suffering from COVID-19 pneumonia – a case report

Rajpal

Kumar

Saurabh

et al. 2023

Access Microbiology

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The coronavirus disease 2019 (COVID-19) pandemic has manifested as a multifaceted paradigm but has primarily affected the respiratory system. Though a rare sequela after-COVID-19, we present a case of cavitary lung lesion in an adult patient, which manifested with common symptoms such as fever, cough and dyspnoea during the post-COVID-19 recovery period. Aspergillus flavus and Enterobacter cloacae were found to be the main causative organisms. Fungal and bacterial coinfection may be thought of in similar situations and appropriate treatment may be given to prevent further morbidity and mortality.

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Section: Discussionmentioning

confidence: 99%

Post-COVID-19 cavitary lung lesion due to Aspergillus flavus and Enterobacter cloacae in a patient suffering from COVID-19 pneumonia – a case report

Rajpal

Kumar

Saurabh

et al. 2023

Access Microbiology

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“…Diffuse alveolar damage is the main histopathological manifestation of lung cavities and can result in persistent SARS-CoV-2 RNA in the lungs. Regular screening for bacterial and fungal co-infections is recommended for COVID-19 patients [11]. Therefore, it is essential for physicians to be aware of the possibility of lung cavity formation in COVID-19 patients with respiratory symptoms, as it can indicate a more severe disease course and require appropriate management.…”

Section: Introductionmentioning

confidence: 99%

Pulmonary Cavitation as a Complication of COVID-19: Case Series and a Brief Review of the Literature

Aghamohammadi,

Ghodrati,

Etemadi Sh

et al. 2024

AMH

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The COVID-19 pandemic has led to an unprecedented surge in respiratory illness cases worldwide. Although uncommon, pulmonary cavitation has been reported as a potential complication of COVID-19. This case series report describes four cases of COVID-19 patients with lung cavities, highlighting the various causes and clinical manifestations of this complication, and a review of the literature on the presence of lung cavities in COVID-19 patients. In two cases, the cavities were most likely due to secondary bacterial superinfections, with one case being complicated by multi-drug-resistant bacteria. Another case developed cavities secondary to a fungal infection, while the third case was directly caused by SARS-CoV-2 invasion in the lungs. The presence of cavities with or without air-fluid level or pneumothorax in COVID-19 patients should be considered as a potential complication of this infection, especially in those with respiratory symptoms. Physicians should remain vigilant for the development of pulmonary cavitation in COVID-19 patients, particularly those receiving high doses of steroids. Additionally, spontaneous pneumothorax should be considered an alarming sign in COVID-19 patients.

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“…Moreover, bacterial communities and biofilms can impact the infection of mammals by viruses. For example, biofilms found in bat caves allow for the protection and spread of SARS-CoV-2 among the bat population [ 38 , 42 , 43 ]. Furthermore, from a biophysics perspective, viral stability could also be enhanced by the thin liquid extracellular polymeric substances (EPS) produced by bacterial biofilms [ 33 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Delta variant remains viable in environmental biofilms found in meat packaging plants

Featherstone,

Mathijssen,

Brown

et al. 2024

PLoS ONE

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To determine why SARS-CoV-2 appears to thrive specifically well in meat packaging plants, we used SARS-CoV-2 Delta variant and meat packaging plant drain samples to develop mixed-species biofilms on materials commonly found within meat packaging plants (stainless steel (SS), PVC, and ceramic tile). Our data provides evidence that SARS-CoV-2 Delta variant remained viable on all the surfaces tested with and without an environmental biofilm after the virus was inoculated with the biofilm for 5 days at 7°C. We observed that SARS-CoV-2 Delta variant was able to remain infectious with each of the environmental biofilms by conducting plaque assay and qPCR experiments, however, we detected a significant reduction in viability post-exposure to Plant B biofilm on SS, PVC, and on ceramic tile chips, and to Plant C biofilm on SS and PVC chips. The numbers of viable SARS-CoV-2 Delta viral particles was 1.81–4.57-fold high than the viral inoculum incubated with the Plant B and Plant C environmental biofilm on SS, and PVC chips. We did not detect a significant difference in viability when SARS-CoV-2 Delta variant was incubated with the biofilm obtained from Plant A on any of the materials tested and SARS-CoV-2 Delta variant had higher plaque numbers when inoculated with Plant C biofilm on tile chips, with a 2.75-fold difference compared to SARS-CoV-2 Delta variant on tile chips by itself. In addition, we detected an increase in the biofilm biovolume in response to SARS-CoV-2 Delta variant which is also a concern for food safety due to the potential for foodborne pathogens to respond likewise when they come into contact with the virus. These results indicate a complex virus-environmental biofilm interaction which correlates to the different bacteria found in each biofilm. Our results also indicate that there is the potential for biofilms to protect SARS-CoV-2 from disinfecting agents and remaining prevalent in meat packaging plants.

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Biofilms possibly harbor occult SARS-CoV-2 may explain lung cavity, re-positive and long-term positive results

Cited by 3 publications

References 83 publications

Post-COVID-19 cavitary lung lesion due to Aspergillus flavus and Enterobacter cloacae in a patient suffering from COVID-19 pneumonia – a case report

Post-COVID-19 cavitary lung lesion due to Aspergillus flavus and Enterobacter cloacae in a patient suffering from COVID-19 pneumonia – a case report

Pulmonary Cavitation as a Complication of COVID-19: Case Series and a Brief Review of the Literature

SARS-CoV-2 Delta variant remains viable in environmental biofilms found in meat packaging plants

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