Biofilms of Candida albicans and Streptococcus sanguinis and their susceptibility to antimicrobial effects of photodynamic inactivation

Palma, Ana Luiza do Rosário; Ramos, Lucas de Paula; Domingues, Nádia; Back-Brito, Graziella Nuernberg; Oliveira, Luciane Dias de; Pereira, Cristiane Aparecida; Jorge, Antônio Olavo Cardoso

doi:10.1016/j.pdpdt.2018.07.003

Cited by 12 publications

(5 citation statements)

References 34 publications

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“…18 Some scholars also use incandescent lamp as the light source and believe that PDT has a signi¯cant sterilization e®ect only when the energy density is above 500 J/cm 2 . 19,20 The energy density of di®erent light sources is mainly due to the di®erent absorption e±ciency of photosensitizer to di®erent wavelengths of light. In this experiment, a more stable and more e±cient laser was used as the light source, so the 15.2 J/cm 2 group and the 30.4 J/cm 2 group were set up to compare the e®ect of aPDT therapy under di®erent energy densities.…”

Section: Discussionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

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Section: Discussionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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“…Regarding therapeutic responses applying light, Pourhajibagher et al [131] reported that an acrylic resin containing Undaria pinnatifida, using photo-activation with LED, has antimicrobial properties against planktonic and mixed biofilms forms of cariogenic microorganisms (S. mutans, S. sanguinis, and Lactobacillus acidophilus) and C. albicans, even at the lowest concentration. Correspondingly, photodynamic inactivation (PDI) on single-and multi-species biofilms of C. albicans and S. sanguinis showed reductions of 1.07 (single) and 0.39 log 10 (mixed), demonstrating that PDI is a possible way to control these clinically important microorganisms [137]. In another work, Diogo et al used photodynamic therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e 6 Me) activated by red light against monospecies and mixed-species biofilms of E. faecalis and C. albicans.…”

Section: Oral Disease Managementmentioning

confidence: 99%

Candida spp./Bacteria Mixed Biofilms

Rodrigues

Gomes

Rodrigues

2019

JoF

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The ability to form biofilms is a common feature of microorganisms, such as bacteria or fungi. These consortiums can colonize a variety of surfaces, such as host tissues, dentures, and catheters, resulting in infections highly resistant to drugs, when compared with their planktonic counterparts. This refractory effect is particularly critical in polymicrobial biofilms involving both fungi and bacteria. This review emphasizes Candida spp.-bacteria biofilms, the epidemiology of this community, the challenges in the eradication of such biofilms, and the most relevant treatments.

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“…Although the example above suggests that PDDI efficacy may increase as the molecular weight of the photosensitizer decreases, the modest photoinactivations observed with methylene blue (PS 18 ), curcumin (PS 14 ) [ 229 , 230 ], and erythrosine (PS 44 ) [ 231 ], advise against generalizations. These small photosensitizers are monocationic, neutral and dianionic, respectively.…”

Section: Fungi and Biofilmsmentioning

confidence: 99%

Photodynamic disinfection and its role in controlling infectious diseases

Aroso

Schaberle

Arnaut

et al. 2021

Photochem Photobiol Sci

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Photodynamic therapy is witnessing a revival of its origins as a response to the rise of multi-drug resistant infections and the shortage of new classes of antibiotics. Photodynamic disinfection (PDDI) of microorganisms is making progresses in preclinical models and in clinical cases, and the perception of its role in the clinical armamentarium for the management of infectious diseases is changing. We review the positioning of PDDI from the perspective of its ability to respond to clinical needs. Emphasis is placed on the pipeline of photosensitizers that proved effective to inactivate biofilms, showed efficacy in animal models of infectious diseases or reached clinical trials. Novel opportunities resulting from the COVID-19 pandemic are briefly discussed. The molecular features of promising photosensitizers are emphasized and contrasted with those of photosensitizers used in the treatment of solid tumors. The development of photosensitizers has been accompanied by the fabrication of a variety of affordable and customizable light sources. We critically discuss the combination between photosensitizer and light source properties that may leverage PDDI and expand its applications to wider markets. The success of PDDI in the management of infectious diseases will ultimately depend on the efficacy of photosensitizers, affordability of the light sources, simplicity of the procedures, and availability of fast and efficient treatments. Graphic abstract

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Biofilms of Candida albicans and Streptococcus sanguinis and their susceptibility to antimicrobial effects of photodynamic inactivation

Cited by 12 publications

References 34 publications

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Candida spp./Bacteria Mixed Biofilms

Photodynamic disinfection and its role in controlling infectious diseases

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