Biofilms in the Food Industry

Agle, Meredith E.

doi:10.1002/9780470277782.ch1

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…Unlike Coquet et al (1998), we simply used the alginate matrix without bacterial growth and thereby avoided interference with MNV-1 quantification by the cell culture method. By preparing a thinner biofilm (1 mm or less) than those generally used (3 mm) (Coquet et al 1998;Jouenne et al 1994), we examined biofilm conditions closer to those found in the food industry, where thicknesses are usually less than 50 lm (Agle 2007). Our use of 90 % matrix and 10 % MNV-1 lysate reproduced conditions described previously (85 % matrix, 15 % microorganisms) for imitating natural biofilms (Agle 2007).…”

Section: Discussionmentioning

confidence: 90%

“…By preparing a thinner biofilm (1 mm or less) than those generally used (3 mm) (Coquet et al 1998;Jouenne et al 1994), we examined biofilm conditions closer to those found in the food industry, where thicknesses are usually less than 50 lm (Agle 2007). Our use of 90 % matrix and 10 % MNV-1 lysate reproduced conditions described previously (85 % matrix, 15 % microorganisms) for imitating natural biofilms (Agle 2007). We thus demonstrated that PAA and PPA diffused unhindered into the alginate matrix and inactivated the virus lodged within the film.…”

Section: Discussionmentioning

confidence: 99%

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Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

2014

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This study was conducted to evaluate the efficacy of four different peroxyacids, namely peracetic (PAA), perpropionic (PPA), perlactic (PLA), and percitric (PCA) for inactivating viruses in suspension or attached to stainless steel or polyvinyl chloride surfaces. The test virus was a proxy for human norovirus, namely murine norovirus 1. Plaque-forming units in suspension (10(7) per mL) were treated with 50-1,000 mg L(-1) peroxyacid (equilibrium mixture of organic acid, hydrogen peroxide, peroxyacid, and water) for 1-10 min. Inactivation was measured by plaque assay. PAA and PPA were the most effective, with a 5 min treatment at 50 mg L(-1) being sufficient to reduce viral titer by at least 3.0 log10, whether the virus was in suspension or attached to stainless steel or polyvinyl chloride disks under clean or fouled conditions. Combinations of organic acid and hydrogen peroxide were found ineffective. Similar inactivation was observed in the case of virus in artificial biofilm (alginate gel). These short super-oxidizers could be used for safe inactivation of human noroviruses in water or on hard surfaces.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

2014

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“…Attachment of Salmonella to food-processing surfaces and subsequent development of biofilms may have significant economic and public health consequences (Donlan & Costerton, 2002;Burmolle et al, 2006;Agle, 2007). It is suggested that Salmonella biofims adapt structurally to changes in the medium osmolarity and nutrients (Lapidot et al, 2006;Mangalappalli-Illathu et al, 2008).…”

Section: Opgs and Biofilm Formationmentioning

confidence: 99%

Osmoregulated periplasmic glucans are needed for competitive growth and biofilm formation bySalmonella entericaserovar Typhimurium in leafy-green vegetable wash waters and colonization in mice

Liu

Tan

Jun

et al. 2009

FEMS Microbiology Letters

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Osmoregulated periplasmic glucans (OPGs) are major periplasmic constituents of Gram-negative bacteria. The role of OPGs has been postulated in symbiotic as well as pathogenic host-microorganism interactions. Here, we report the role of OPGs from Salmonella enterica serovar Typhimurium during growth and biofilm formation in leafy-green vegetable wash water. The opgGH mutant strain, which was defective in OPG biosynthesis, initiated the growth at a slower rate in wash waters obtained from spinach, lettuce and green collard and severely impaired biofilm formation. The lack of OPG synthesis did not influence biofilm formation by the opgGH mutant in low-nutrient low-osmolarity laboratory media. In coculture experiments initiated with equal proportions of cells, the opgGH mutant was outnumbered by the wild-type strain under the planktonic as well as the biofilm growth conditions. The opgGH mutant strain poorly colonized mouse organs when introduced orally along with the wild-type strain. This is the first report demonstrating the role of OPGs of Salmonella in competitive colonization of biofilms, planktonic cultures and mouse organs.

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“…The developed microbial biofilms are generally difficult to remove and inactivate, since the structural characteristics of biofilms constituted by the microbial cells and the produced extracellular polymeric substance (EPS) during biofilm formation after cell attachment play protecting roles against various chemical and physical stresses (Agle, 2007;Costerton, Lewandowski, Caldwell, Korber, & Lappin-Scott, 1995;Kumar & Anand, 1998). Therefore, clarification of structural characteristics of biofilm is extremely important for conducting more effective removal and inactivation treatment.…”

Section: Introductionmentioning

confidence: 99%

Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm

et al. 2012

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Biofilms in the Food Industry

Cited by 11 publications

References 31 publications

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

Osmoregulated periplasmic glucans are needed for competitive growth and biofilm formation bySalmonella entericaserovar Typhimurium in leafy-green vegetable wash waters and colonization in mice

Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm

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