Biofilms in chronic infections – a matter of opportunity – monospecies biofilms in multispecies infections

Burmølle, Mette; Thomsen, Trine Rolighed; Fazli, Mustafa; Dige, Irene; Christensen, Lise; Homøe, Preben; Tvede, Michael; Nyvad, Bente; Tolker‐Nielsen, Tim; Givskov, Michael; Moser, Claus; Kirketerp‐Møller, Klaus; Johansen, Helle Krogh; Høiby, Niels; Jensen, Peter Østrup; Sørensen, Søren J.; Bjarnsholt, Thomas

doi:10.1111/j.1574-695x.2010.00714.x

Cited by 369 publications

(300 citation statements)

References 87 publications

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“…Second, the defect in biofilm formation was another in vitro phenotype exhibited by the purL mutant (Figure 2c). Bacterial biofilm formation is one of the adaptation mechanisms to various environments and has been reported to be required for chronic infections and symbioses (Costerton et al, 2003;Ramey et al, 2004;Visick, 2009;An and Grewal, 2010;Burmølle et al, 2010;Morris and Visick, 2010;Rinaudi and Giordano, 2010;Maltz et al, 2012). In squid-Vibrio symbiosis, Vibrio fischeri uses a transient biofilm to colonize its host, Euprymna scolope (Visick, 2009;Morris and Visick, 2010).…”

Section: Discussionmentioning

confidence: 99%

Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug

et al. 2013

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The Riptortus–Burkholderia symbiotic system represents a promising experimental model to study the molecular mechanisms involved in insect–bacterium symbiosis due to the availability of genetically manipulated Burkholderia symbiont. Using transposon mutagenesis screening, we found a symbiosis-deficient mutant that was able to colonize the host insect but failed to induce normal development of host’s symbiotic organ. The disrupted gene was identified as purL involved in purine biosynthesis. In vitro growth impairment of the purL mutant and its growth dependency on adenine and adenosine confirmed the functional disruption of the purine synthesis gene. The purL mutant also showed defects in biofilm formation, and this defect was not rescued by supplementation of purine derivatives. When inoculated to host insects, the purL mutant was initially able to colonize the symbiotic organ but failed to attain a normal infection density. The low level of infection density of the purL mutant attenuated the development of the host’s symbiotic organ at early instar stages and reduced the host’s fitness throughout the nymphal stages. Another symbiont mutant-deficient in a purine biosynthesis gene, purM, showed phenotypes similar to those of the purL mutant both in vitro and in vivo, confirming that the purL phenotypes are due to disrupted purine biosynthesis. These results demonstrate that the purine biosynthesis genes of the Burkholderia symbiont are critical for the successful accommodation of symbiont within the host, thereby facilitating the development of the host’s symbiotic organ and enhancing the host’s fitness values.

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Section: Discussionmentioning

confidence: 99%

Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug

et al. 2013

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“…2) and this might explain how the bacteria survive inside the wound bed. In chronic wounds individual micro colonies might exist that only consist of single species, and it is very likely that mono-and polymicrobial biofilms can be found in the same ulcer, but the importance and relevance of this has yet to be established (Kirketerp-Moller et al, 2008;Burmølle et al, 2010). There are also biofilms in healing wounds, and other factors like host response, virulence and antibiotic resistance seem important for the fate of the wound.…”

Section: Microbial Diversitymentioning

confidence: 99%

Influence of Microorganisms on the Healing of Skin Grafts from Chronic Venous Leg Wounds

Wolff¹,

Bjarnsholt²,

Kirketerp‐Møller³

et al. 2011

Skin Grafts - Indications, Applications and Current Research

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“…Chronic wounds infecting bacteria are generally present in biofilm form 32 .Therefore pharmaceutical products with biofilm disrupting activity are extremely necessary for chronic wound treatment. It was demonstrated that the biofilm disruption showed by LAPS 7,8 is due to synergism between surfactants (Tween 80, 1-mono-linolein, 1,2-dipalmitin and distearin) 8,33 , an extracellular DNAase 8,19 and variations in the ionic strength (Na, K and Mg salts) 8,34 .…”

Section: Supernatants Biofilm Disruption Activitymentioning

confidence: 99%

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Lindon¹,

Cruz²,

Cabral³

et al. 2016

IJPSR

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ABSTRACT:Lactobacillus plantarum culture supernatants (LAPS) have antimicrobial, pro-healing and anesthetic properties, so our medical team applied whole cultures and LAPS in chronic wounds with encouraging results. Metabolites responsible for LAPS properties and its action mechanism were previously determined. Objective: to design methods and culture media to increase the production of LAPS metabolites and optimize it´s therapeutic effectiveness. Modifications in MRS broth composition were made and L. plantarum was cultivated in the resulting different media with different physicochemical conditions to obtain modified supernatants (LAPS m ). Antimicrobial and anti-biofilm activity (crystal violet) of LAPS m on strains typically isolated from chronic wounds (Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis and Serratiamarcescens), was tested. Concentration of barbiturates in LAPS m was quantified by GCMS and its relative anesthetic potency in healthy volunteers was evaluated. DNAase and auto inducers type 2biological activity in LAPS m were quantified. In addition, a safety assessment of LAPS m application on healthy skin of 200 volunteers was performed. LAPS m obtained from media with high concentrations of yeast extract possessed the greatest anesthetic power. Those containing greater amount of proteins, cations and surfactants had the highest capacity of biofilm disruption (p<0.01). When glucose and galactose concentration were increased in media, LAPS m had the greatest antimicrobial power (p<0.05). Finally, there is great safety in the application of LAPS m as no edema or erythema was observed. The results obtained will allow the manufacture of LAPS m with greater therapeutic effectiveness and even custom properties for each type of wound.

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Biofilms in chronic infections – a matter of opportunity – monospecies biofilms in multispecies infections

Cited by 369 publications

References 87 publications

Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug

Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug

Influence of Microorganisms on the Healing of Skin Grafts from Chronic Venous Leg Wounds

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