Biofilms and their impact on the food industry

Olanbiwoninu, A.A.; Popoola, B.M.

doi:10.1016/j.sjbs.2022.103523

Cited by 14 publications

(3 citation statements)

References 61 publications

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“…In the context of hospital infection control, biofilms formed on medical devices and wound surfaces greatly impact patient health [ 57 ]. Furthermore, within the food industry, microbial biofilms often develop on food packaging, posing risks to food safety [ 58 , 59 ]. Thankfully, numerous studies have reported the antimicrobial biofilm activity of various nanoparticles [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

Liu,

Huang,

Chen

et al. 2023

J Nanobiotechnol

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Background Carbapenem-resistant Enterobacteriaceae (CRE) present substantial challenges to clinical intervention, necessitating the formulation of novel antimicrobial strategies to counteract them. Nanomaterials offer a distinctive avenue for eradicating bacteria by employing mechanisms divergent from traditional antibiotic resistance pathways and exhibiting reduced susceptibility to drug resistance development. Non-caloric artificial sweeteners, commonly utilized in the food sector, such as saccharin, sucralose, acesulfame, and aspartame, possess structures amenable to nanomaterial formation. In this investigation, we synthesized gold nanoparticles decorated with non-caloric artificial sweeteners and evaluated their antimicrobial efficacy against clinical CRE strains. Results Among these, gold nanoparticles decorated with aspartame (ASP_Au NPs) exhibited the most potent antimicrobial effect, displaying minimum inhibitory concentrations ranging from 4 to 16 µg/mL. As a result, ASP_Au NPs were chosen for further experimentation. Elucidation of the antimicrobial mechanism unveiled that ASP_Au NPs substantially elevated bacterial reactive oxygen species (ROS) levels, which dissipated upon ROS scavenger treatment, indicating ROS accumulation within bacteria as the fundamental antimicrobial modality. Furthermore, findings from membrane permeability assessments suggested that ASP_Au NPs may represent a secondary antimicrobial modality via enhancing inner membrane permeability. In addition, experiments involving crystal violet and confocal live/dead staining demonstrated effective suppression of bacterial biofilm formation by ASP_Au NPs. Moreover, ASP_Au NPs demonstrated notable efficacy in the treatment of Galleria mellonella bacterial infection and acute abdominal infection in mice, concurrently mitigating the organism's inflammatory response. Crucially, evaluation of in vivo safety and biocompatibility established that ASP_Au NPs exhibited negligible toxicity at bactericidal concentrations. Conclusions Our results demonstrated that ASP_Au NPs exhibit promise as innovative antimicrobial agents against clinical CRE. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

Liu,

Huang,

Chen

et al. 2023

J Nanobiotechnol

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“…In the context of hospital infection control, bio lms formed on medical devices and wound surfaces greatly impact patient health [52] . Furthermore, within the food industry, microbial bio lms often develop on food packaging, posing risks to food safety [53,54] . Thankfully, numerous studies have reported the antimicrobial bio lm activity of various nanoparticles [55] .…”

Section: In Vitro Bio Lm Inhibition Study Of Asp_au Npsmentioning

confidence: 99%

A Potential Strategy against Clinical Carbapenem-Resistant Enterobacteriaceae: Antimicrobial Activity Study of Sweetener-Decorated Gold Nanoparticles in Vitro and in Vivo

Liu,

Huang,

Chen

et al. 2023

Preprint

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Background Carbapenem-resistant Enterobacteriaceae (CRE) present substantial challenges to clinical intervention, necessitating the formulation of novel antimicrobial strategies to counteract them. Nanomaterials offer a distinctive avenue for eradicating bacteria by employing mechanisms divergent from traditional antibiotic resistance pathways and exhibiting reduced susceptibility to drug resistance development. Non-caloric artificial sweeteners, commonly utilized in the food sector, such as saccharin, sucralose, acesulfame, and aspartame, possess structures amenable to nanomaterial formation. In this investigation, we synthesized gold nanoparticles decorated with non-caloric artificial sweeteners and evaluated their antimicrobial efficacy against clinical CRE strains. Results Among these, gold nanoparticles decorated with aspartame (ASP_Au NPs) exhibited the most potent antimicrobial effect, displaying minimum inhibitory concentrations ranging from 4 to 16 µg/mL. As a result, ASP_Au NPs were chosen for further experimentation. Elucidation of the antimicrobial mechanism unveiled that ASP_Au NPs substantially elevated bacterial reactive oxygen species (ROS) levels, which dissipated upon ROS scavenger treatment, indicating ROS accumulation within bacteria as the fundamental antimicrobial modality. Furthermore, findings from membrane potential assessments suggested that ASP_Au NPs may represent a potential antimicrobial modality via depolarization of bacterial membranes. In addition, experiments involving crystal violet demonstrated effective suppression of bacterial biofilm formation by ASP_Au NPs. Moreover, ASP_Au NPs demonstrated notable efficacy in the treatment of Galleria mellonella bacterial infection and acute abdominal infection in mice, concurrently mitigating the organism's inflammatory response. Crucially, evaluation of in vivo safety and biocompatibility established that ASP_Au NPs exhibited negligible toxicity at bactericidal concentrations. Conclusions Our results demonstrated that ASP_Au NPs exhibit promise as innovative antimicrobial agents against clinical CRE.

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“…Phylogenetic analyses showed the close evolutionary relationships of C. maltosa with C. tropicaiis , C. viswanathii , C. aibicans , C. parapsiiosis and C. guiiiiermondii , forming a subgroup within this genus [ 42 ]. Biofilms are also used as a structure-forming matrix for the food industry and sorption of heavy metals in bioremediation [ 43 , 44 , 45 , 46 ]. These materials have good stability and biocompatibility [ 47 ] and have significant potential for various biomedical and biotechnological applications [ 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

Zvonarev,

Trilisenko,

Farofonova

et al. 2023

JoX

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The cell wall of Candida yeast grown on presence of hexadecane as a sole carbon source undergoes structural and functional changes including the formation of specific supramolecular complexes—canals. The canals contain specific polysaccharides and enzymes that provide primary oxidization of alkanes. In addition, inorganic polyphosphate (polyP) was identified in Candida maltosa canals. The aim of the work was a comparative study of the features of cell walls and extracellular structures in yeast C. maltosa, C. albicans and C. tropicalis with special attention to inorganic polyphosphates as possible part of these structures when grown on the widely used xenobiotic hexadecane (diesel fuel). Fluorescence microscopy with DAPI has shown an unusual localization of polyP on the cell surface and in the exovesicles in the three yeast species, when growing on hexadecane. Electron-scanning microscopy showed that the exovesicles were associated with the cell wall and also presented in the external environment probably as biofilm components. Treatment of hexadecane-grown cells with purified Ppx1 polyphosphatase led to the release of phosphate into the incubation medium and the disappearance of polyP in vesicles and cell wall observed using microscopic methods. The results indicate the important role of polyP in the formation of extracellular structures in the Candida yeast when consuming hexadecane and are important for the design of xenobiotic destructors based on yeast or mixed cultures.

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Biofilms and their impact on the food industry

Cited by 14 publications

References 61 publications

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

A Potential Strategy against Clinical Carbapenem-Resistant Enterobacteriaceae: Antimicrobial Activity Study of Sweetener-Decorated Gold Nanoparticles in Vitro and in Vivo

The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

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