Biofilmicidal Efficacy of Five Disinfectants against Campylobacter jejuni on Different Poultry Farm Surfaces

Abstract

Biofilm formation is a complex process, consists of a cord of molecular and physiological events that take place through several stages including adherence, formation of microcolonies, tridimensional structuring, and maturation. Biofilmacts as a reservoir not only for food production facilities but also for animal and poultry husbandry environment and considers a source of unceasing supplier of zoonotic and foodborne microorganisms infecting both hosts and consumers. Among these organisms, Campylobacter jejuni (C. jejuni) colonizes in bird hindgut with high counts. C. jejuni is widely considered as a main cause of gastroenteritis in poultry products consumers and has the ability to form biofilm on surfaces inside poultry farms and slaughterhouses or food contact surfaces. Previous studies detected the biofilm formation and efficacy of sanitizers on food contact surfaces, but fewer examined within and or on the farm components. This study aims to study the ability of C. jejuni to form biofilm on plastic, galvanized wire and concrete surfaces as simulating in poultry farms and evaluate the efficacy of five commercial disinfectants; Pyam ® , Klorsept ® , Calcium hypochlorite ® , PronTech ® and Virukill ®. All which used at two concentrations 0.5% and 1%. The used surfaces showed biofilm formation by different degrees. Our results revealed that Pyam ® , Klorsept ® and Ca. hypochlorite ® showed the highest logarithmic reductions of biofilm count followed by potassium peroxymonosulfate and halide NaCl combination Virukill ® while the lowest reductions were obtained using QAC (Alkyl Dimethyl Benzyl Ammonium Chloride 40% with 60 % Urea, PronTech ®). In conclusions, the use of efficient disinfectants reduces the risk of C. jejuni infections through direct and indirect routes of infection.