Biofilm thickness variability investigated with a laser triangulation sensor

Okkerse, W.J.H.; Ottengraf, Spp Simon; Osinga-Kuipers, B Baukje

doi:10.1002/1097-0290(20001220)70:6<619::aid-bit3>3.0.co;2-4

Cited by 31 publications

(13 citation statements)

References 37 publications

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“…The biofilms were investigated with a cyrosectioning method after embedding of the biofilm. Okkerse et al (2000) used the laser triangulation method and found a value of 0.13 for a 41-day-old dichloromethane-degrading biofilm. Both methods operated with drained biofilms.…”

Section: Structure Parametersmentioning

confidence: 99%

Measuring local flow velocities and biofilm structure in biofilm systems with Magnetic Resonance Imaging (MRI)

Manz

Volke

Goll

et al. 2003

Biotech & Bioengineering

117

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Abstract:The characterization of substrate transport in the bulk phase and in the biofilm matrix is one of the problems which has to be solved for the verification of biofilm models. Additionally, the surface structure of biofilms has to be described with appropriate parameters. Magnetic resonance imaging (MRI) is one of the promising methods for the investigation of transport phenomena and structure in biofilm systems. The MRI technique allows the noninvasive determination of flow velocities and biofilm structures with a high resolution on the sub-millimeter scale. The presented investigations were carried out for defined heterotrophic biofilms which were cultivated in a tube reactor at a Reynolds number of 2000 and 8000 and a substrate load of 6 and 4 g /m 2 d glucose. Magnetic resonance imaging provides both structure data of the biofilm surface and flow velocities in the bulk phase and at the bulk/biofilm interface. It is shown that the surface roughness of the biofilms can be determined in one experiment for the complete cross section of the test tubes both under flow and stagnant conditions. Furthermore, the local shear stress was calculated from the measured velocity profiles. In the investigated biofilm systems the local shear stress at the biofilm surface was up to 3 times higher compared to the mean wall shear stress calculated on the base of the mean flow velocity.

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Section: Structure Parametersmentioning

confidence: 99%

Measuring local flow velocities and biofilm structure in biofilm systems with Magnetic Resonance Imaging (MRI)

Manz

Volke

Goll

et al. 2003

Biotech & Bioengineering

117

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“…Additionally, dehydration may lead to underestimation of the biofilm thickness due to shrinkage. The nondestructive optical methods available are light microscopy (1), a scanner with an image acquisition system (13), a laser triangulation sensor (14), confocal laser scanning microscopy (CLSM) (16), and two-photon excitation microscopy (17), which uses visible, laser, or infrared light to elucidate the three-dimensional structure of a biofilm. For light microscopy, the refractive index of a biofilm is required, which is mostly assumed to be the refractive index of water (1).…”

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confidence: 99%

Low-Load Compression Testing: a Novel Way of Measuring Biofilm Thickness

Paramonova

Jong

Krom

et al. 2007

Appl Environ Microbiol

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Biofilms are complex and dynamic communities of microorganisms that are studied in many fields due to their abundance and economic impact. Biofilm thickness is an important parameter in biofilm characterization. Current methods of measuring biofilm thicknesses have several limitations, including application, availability, and costs. Here, we present low-load compression testing (LLCT) as a new method for measuring biofilm thickness. With LLCT, biofilm thicknesses are measured during compression by inducing small loads, up to 5 Pa, corresponding to 0.1% deformation, making LLCT essentially a nondestructive technique. Comparison of the thicknesses of various bacterial and yeasts biofilms obtained by LLCT and by using confocal laser scanning microscopy (CLSM) resulted in the conclusion that CLSM underestimates the biofilm thickness due to poor penetration of different fluorescent dyes, especially through the thicker biofilms, whereas LLCT does not suffer from this thickness limitation.

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“…The method is rather similar to the method described by Okkerse et al [13]. It is worth noting that the technique adapted is non-destructive and precise, and allows biofilm profiles to be measured over lengths of up to 14 cm in our particular application.…”

Section: Biofilm Thickness Measurementmentioning

confidence: 99%

Biological waste gas treatment with a modified rotating biological contactor. Ι. Control of biofilm growth and long-term performance

Vinage

Rohr

2003

Bioprocess Biosyst Eng

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In this work, we introduce a modified rotating biological contactor (RBC) system and demonstrate its feasibility by applying the newly devised process to the biological treatment of artificial waste gas. In the proposed system, the waste gas is introduced to the bioreactor in the spacings between the rotating discs through a hollow shaft, thus allowing for intimate gas-liquid contact. A 91-l modified RBC containing 20 biofilm support discs 40 cm in diameter was used in the experiments. Toluene was used as the model pollutant, and the system was operated under standard operating conditions for more than one year in order to investigate its long-term performance and assess its ability to control the growth of the biofilm. It was demonstrated that the proposed system allows to efficiently control the growth of the biofilm, thus overcoming the clogging problem inherent in most conventional methods for the biological treatment of waste gas. Moreover, the system was shown to exhibit stationary long-term performance for a period of more than one year, hence indicating its feasibility for industrial application.

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Biofilm thickness variability investigated with a laser triangulation sensor

Cited by 31 publications

References 37 publications

Measuring local flow velocities and biofilm structure in biofilm systems with Magnetic Resonance Imaging (MRI)

Measuring local flow velocities and biofilm structure in biofilm systems with Magnetic Resonance Imaging (MRI)

Low-Load Compression Testing: a Novel Way of Measuring Biofilm Thickness

Biological waste gas treatment with a modified rotating biological contactor. Ι. Control of biofilm growth and long-term performance

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