Biofilm removal from silicone tubing: an assessment of the efficacy of dialysis machine decontamination procedures using an in vitro model

Marion-Ferey, Karine; Pasmore, Mark; Stoodley, Paul; Wilson, Suzanne; Husson, G.; Costerton, J. William

doi:10.1053/jhin.2002.1320

Cited by 83 publications

(51 citation statements)

References 19 publications

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“…Nonenzymatic detergents showed a better inhibition of biofilm formation than enzymatic detergents (368,370). The most efficient methods for biofilm removal were autoclaving and treatment with a concentrated bleach solution (49). High-temperature treatments (80°C to 90°C) were not effective for biofilm removal.…”

Section: Impact Of Biofilm On Endoscope Reprocessingmentioning

confidence: 94%

“…biofilms (47). According to the literature, peracetic acid has the ability to fixate biofilms, and therefore, it can show limited efficacy in biofilm removal from the abiotic surfaces and in killing bacteria within the biofilm (48,49). Vigorous cleaning with brushes or agitated liquids is important to remove as much biofilm as possible (4).…”

Section: Relevance Of Cleaning and Disinfectionmentioning

confidence: 99%

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Transmission of Infection by Flexible Gastrointestinal Endoscopy and Bronchoscopy

et al. 2013

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SUMMARY Flexible endoscopy is a widely used diagnostic and therapeutic procedure. Contaminated endoscopes are the medical devices frequently associated with outbreaks of health care-associated infections. Accurate reprocessing of flexible endoscopes involves cleaning and high-level disinfection followed by rinsing and drying before storage. Most contemporary flexible endoscopes cannot be heat sterilized and are designed with multiple channels, which are difficult to clean and disinfect. The ability of bacteria to form biofilms on the inner channel surfaces can contribute to failure of the decontamination process. Implementation of microbiological surveillance of endoscope reprocessing is appropriate to detect early colonization and biofilm formation in the endoscope and to prevent contamination and infection in patients after endoscopic procedures. This review presents an overview of the infections and cross-contaminations related to flexible gastrointestinal endoscopy and bronchoscopy and illustrates the impact of biofilm on endoscope reprocessing and postendoscopic infection.

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Section: Impact Of Biofilm On Endoscope Reprocessingmentioning

confidence: 94%

Section: Relevance Of Cleaning and Disinfectionmentioning

confidence: 99%

Transmission of Infection by Flexible Gastrointestinal Endoscopy and Bronchoscopy

et al. 2013

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“…18,19 No other technique can provide these two attributes simultaneously, which are vitally important for investigating biofilm formation and removal. 20 The large area is necessary because the simultaneous movement of hundreds or thousands of cells, as well as the insoluble polysaccharides and proteins excreted by the cells, in a biofilm must be tracked during each stage of the biofilm life cycle. 14 Several techniques have been developed to address these unique challenges.…”

Section: A Biofilm Analysismentioning

confidence: 99%

Using surface plasmon resonance imaging to study bacterial biofilms

et al. 2014

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This paper describes the use of Surface Plasmon Resonance imaging (SPRi) as an emerging technique to study bacterial physiology in real-time without labels. The overwhelming majority of bacteria on earth exist in large multicellular communities known as biofilms. Biofilms are especially problematic because they facilitate the survival of pathogens, leading to chronic and recurring infections as well as costly industrial complications. Monitoring biofilm accumulation and removal is therefore critical in these and other applications. SPRi uniquely provides label-free, high-resolution images of biomass coverage on large channel surfaces up to 1 cm 2 in real time, which allow quantitative assessment of biofilm dynamics. The rapid imaging capabilities of this technique are particularly relevant for multicellular bacterial studies, as these cells can swim several body lengths per second and divide multiple times per hour. We present here the first application of SPRi to image Escherichia coli and Pseudomonas aeruginosa cells moving, attaching, and forming biofilms across a large surface. This is also the first time that biofilm removal has been visualized with SPRi, which has important implications for monitoring the biofouling and regeneration of fluidic systems. Initial images of the removal process show that the biofilm releases from the surface as a wave along the direction of the fluid flow. V C 2014 AIP Publishing LLC. [http://dx

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“…Current peracetic acid disinfectants are not used in high concentrations for endoscope reprocessing because of corrosive properties (Gastroenterological Society of Australia, 2006). According to Marion-Ferey et al (2003), peracetic acid had a limited efficacy in biofilm removal from the silicone tubing in haemodialysis systems. Other studies demonstrated the ability of peracetic acid disinfectants to fix the E. coli biofilm and blood on to artificial materials (Henoun Loukili et al, 2004;Kampf et al, 2004) Glutaraldehyde is also an effective agent in endoscope disinfection.…”

Section: Removal Of Biofilmsmentioning

confidence: 99%