Biofilm Quantification on Nasolacrimal Silastic Stents After Dacryocystorhinostomy

Murphy, Jae; Ali, M. Javed; Psaltis, Alkis J.

doi:10.1097/iop.0000000000000361

Cited by 35 publications

(26 citation statements)

References 24 publications

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“…Although biofilms on the surface of the lacrimal stents is a well-known phenomenon and may increased the probability of infection,21, 22, 23 in the presence of the biofilms the culture may be negative 24, 25. Murphy J and colleagues reported that biofilm was present in nasolacrimal stents after 4 weeks, however we suggested that it's better to postponed the intraocular surgery until the removal of silicone tube.…”

Section: Discussionmentioning

confidence: 73%

Conjunctival bacterial flora in fellow eyes of patients with unilateral nasolacrimal duct obstruction and its changes after successful dacryocystorhinostomy surgery

Eshraghi

Alemzadeh

Abedinifar

2017

Journal of Current Ophthalmology

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PurposeTo evaluate the results of conjunctival culture in fellow eyes of patients with unilateral nasolacrimal duct obstruction (NLDO) and its changes after successful dacryocystorhinostomy (DCR) surgery.MethodsIn this prospective study, 71 adult patients with unilateral NLDO and 41 age and sex-matched controls without NLDO were evaluated. The patients were divided into 2 groups based on clinical examination; group A with purulent regurgitation and group B without purulent regurgitation. They all underwent DCR. Before DCR surgery, microbiologic specimens were taken bilaterally from the conjunctiva of both eyes. Postoperative conjunctival sampling was continued weekly until the culture became negative or the colony count reached to the range of the control group.ResultsThere were 38 and 33 patients in groups A and B, respectively. Silicone tube was inserted for 17 patients (23.9%). The culture was positive for bacterial growth in 56 fellow eyes (79%). The conjunctival culture in the control group was positive in 17 eyes (41.4%). The mean count of colonies in a sample unit was 624.73 ± 2412.31, 195.75 ± 407.56, and 9.5 ± 1.5 for group A, group B, and controls, respectively. The mean time of normalization of specimens was 1.43 ± 0.69 weeks (range 1–4). Higher colony count at baseline and presence of silicone tube in infected eye were significantly associated with longer normalization time for fellow eye (P < 0.001 and P = 0.003 respectively).ConclusionsThis study suggests that after successful DCR surgery, a waiting period of 4 weeks is needed for conjunctival bacterial cultures to become negative or reach the level of the normal eyes, in the fellow eyes of patients with unilateral NLDO.

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Section: Discussionmentioning

confidence: 73%

Conjunctival bacterial flora in fellow eyes of patients with unilateral nasolacrimal duct obstruction and its changes after successful dacryocystorhinostomy surgery

Eshraghi

Alemzadeh

Abedinifar

2017

Journal of Current Ophthalmology

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“…One common approach is simple thresholding, which is effective when there are differences in the pixel intensity value between biofilm and background. Many studies investigating biofilm removal have used thresholding to segment images of biofilm from photographs and light microscopy images, however typically manual thresholding has been employed81726, which is subjective leading to large errors and making intra-operator reproducibility impossible. Other studies using automatic thresholding have failed to specify the exact method of segmentation or validate its accuracy71621.…”

mentioning

confidence: 99%

A quantitative method to measure biofilm removal efficiency from complex biomaterial surfaces using SEM and image analysis

Vyas

Sammons

Addison

et al. 2016

Sci Rep

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Biofilm accumulation on biomaterial surfaces is a major health concern and significant research efforts are directed towards producing biofilm resistant surfaces and developing biofilm removal techniques. To accurately evaluate biofilm growth and disruption on surfaces, accurate methods which give quantitative information on biofilm area are needed, as current methods are indirect and inaccurate. We demonstrate the use of machine learning algorithms to segment biofilm from scanning electron microscopy images. A case study showing disruption of biofilm from rough dental implant surfaces using cavitation bubbles from an ultrasonic scaler is used to validate the imaging and analysis protocol developed. Streptococcus mutans biofilm was disrupted from sandblasted, acid etched (SLA) Ti discs and polished Ti discs. Significant biofilm removal occurred due to cavitation from ultrasonic scaling (p < 0.001). The mean sensitivity and specificity values for segmentation of the SLA surface images were 0.80 ± 0.18 and 0.62 ± 0.20 respectively and 0.74 ± 0.13 and 0.86 ± 0.09 respectively for polished surfaces. Cavitation has potential to be used as a novel way to clean dental implants. This imaging and analysis method will be of value to other researchers and manufacturers wishing to study biofilm growth and removal.

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“…Once infection occurs, to treat infection and prevent recurrences, the prosthetic medical devices must be removed and antibiotics must be used at stronger doses or more often. It has been reported that if lacrimal stents are left longer than 1 month, biofilms may influence postoperative healing and the ultimate outcome [ 34 ]. Our results also support the use of prophylactic antibiotics after surgery.…”

Section: Discussionmentioning

confidence: 99%

Species-specific characteristics of the biofilm generated in silicone tube: an in vitro study

2018

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BackgroundTo investigate characteristics of biofilm which is usually found in silicone tube for nasolacrimal duct surgery and can be the root of chronic bacterial infections eventually resulted in surgical failure.MethodsTo form a biofilm, sterile silicone tube was placed in culture media of Staphylococcus aureus, Corynebacterium matruchotii, Pseudomonas aeruginosa, or Streptococcus pneumonia. Biofilms formed on these silicone tubes were fixed with 95% ethanol and stained with 0.1% crystal violet. After staining, the optical densities of biofilms were measured using spectrophotometer on a weekly basis for 12 weeks.ResultsStaphylococcus aureus group and Pseudomonas aeruginosa group formed significantly more amounts of biofilms compared to the control group. The maximum optical densities of the two groups were found on week 3–4 followed by a tendency of decrease afterwards. However, the amounts of biofilms formed in other groups of silicone tubes were not statistically significant from that of the control group.ConclusionsBacterial species that could form biofilm on silicone tube included Staphylococcus aureus (week 3) and Pseudomonas aeruginosa (Week 4). It is important to first consider that the cause of infection around 1 month after silicone tube intubation can be Staphylococcus aureus and Pseudomonas aeruginosa.

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Biofilm Quantification on Nasolacrimal Silastic Stents After Dacryocystorhinostomy

Abstract: This is the first study to report the quantification of biomass on lacrimal stents. The presence of biofilms on lacrimal stents after DCR is a common finding but this need not necessarily translate to postoperative clinical infection.

Cited by 35 publications

References 24 publications

Conjunctival bacterial flora in fellow eyes of patients with unilateral nasolacrimal duct obstruction and its changes after successful dacryocystorhinostomy surgery

Conjunctival bacterial flora in fellow eyes of patients with unilateral nasolacrimal duct obstruction and its changes after successful dacryocystorhinostomy surgery

A quantitative method to measure biofilm removal efficiency from complex biomaterial surfaces using SEM and image analysis

Species-specific characteristics of the biofilm generated in silicone tube: an in vitro study

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