Biofilm production by clinical strains of<i>Acinetobacter baumannii</i>isolated from patients hospitalized in two tertiary care hospitals

Wróblewska, Marta; Sawicka-Grzelak, A.; Marchel, Halina; Łuczak, M.; Sivan, Alex

doi:10.1111/j.1574-695x.2008.00403.x

Cited by 43 publications

(30 citation statements)

References 13 publications

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“…Infections of hospitalized patients with Acinetobacter spp., often preceded by colonization, are frequently associated with invasive procedures and implantable medical devices. This process may be facilitated by the ability of a strain to form a biofilm (Wroblewska et al, 2008). It has become apparent that biofilm formation is a common trait of A. baumannii clinical isolates (McQueary and Actis, 2011).…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Imane¹,

Hassaïne²,

Bellifa³

et al. 2014

Afr. J. Microbiol. Res.

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Acinetobacter baumannii is an opportunistic pathogen responsible for nosocomial infections due to biofilm formation on the surface of implantable medical devices. Thirty (30) strains of A. baumannii were isolated from medical devices and tested for their ability to form a biofilm. The factors that may influence this process, such as the hydrophobicity of the bacterial wall, temperature, duration of implantation and the nature of the medical device, were also investigated. Strains were able to form a biofilm; however this process was more substantial at 30°C than at 37°C and was maximal after 96 h of incubation. Strains seem to adhere better to silicone and latex than to polyvinylchloride (PVC) and no apparent relationship was found between hydrophobicity and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Imane¹,

Hassaïne²,

Bellifa³

et al. 2014

Afr. J. Microbiol. Res.

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“…Investigation of characteristics (e.g., resistance to disinfectants or drying, biofilm formation, adherence to human cells, presence of iron-uptake systems) that could favor particular lineages as colonizers or pathogens in hospitals has so far failed to distinguish isolates belonging to successful epidemic lineages from other genotypes (29)(30)(31)(32)(33). However, multidrug resistance to antimicrobial agents appears to be strongly associated with the international epidemic lineages (23), and may therefore represent one of the main reasons for their evolutionary success.…”

Section: Reasons For the Success Of The Epidemic Clonal Lineagesmentioning

confidence: 99%

Acinetobacter infection – an emerging threat to human health

2011

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SummaryThe genus Acinetobacter comprises a complex and heterogeneous group of bacteria, many of which are capable of causing a range of opportunistic, often catheter-related, infections in humans. However, Acinetobacter baumannii, as well as its close relatives belonging to genomic species 3 (''Acinetobacter pittii'') and 13TU (''Acinetobacter nosocomialis''), are important nosocomial pathogens, often associated with epidemic outbreaks of infection, that are only rarely found outside of a clinical setting. These organisms are frequently pandrug-resistant and are capable of causing substantial morbidity and mortality in patients with severe underlying disease, both in the hospital and in the community. Several epidemic clonal lineages of A. baumannii have disseminated worldwide and seem to have a selective advantage over non-epidemic strains. The reasons for the success of these epidemic lineages remain to be elucidated, but could be related to the potential of these organisms to achieve very dynamic reorganization and rapid evolution of their genome, including the acquisition and expression of additional antibiotic resistance determinants, under fluctuating environmental and selective conditions.

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“…The effect of light on biofilm and pellicle formation by the A. baumannii strains used in this study could not be evaluated because of the wide variations observed among them, even under a single culture condition. Such a response is not surprising, considering the significant variations in biofilm phenotypes displayed by different A. baumannii clinical isolates (10,36,53).…”

Section: Vol 192 2010mentioning

confidence: 99%

The Opportunistic Human PathogenAcinetobacter baumanniiSenses and Responds to Light

et al. 2010

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Light is a ubiquitous environmental signal that many organisms sense and respond to by modulating their physiological responses accordingly. While this is an expected response among phototrophic microorganisms, the ability of chemotrophic prokaryotes to sense and react to light has become a puzzling and novel issue in bacterial physiology, particularly among bacterial pathogens. In this work, we show that the opportunistic pathogen Acinetobacter baumannii senses and responds to blue light. Motility and formation of biofilms and pellicles were observed only when bacterial cells were incubated in darkness. In contrast, the killing of Candida albicans filaments was enhanced when they were cocultured with bacteria under light. These bacterial responses depend on the expression of the A. baumannii ATCC 17978 A1S_2225 gene, which codes for an 18.6-kDa protein that contains an N-terminal blue-light-sensing-using flavin (BLUF) domain and lacks a detectable output domain(s). Spectral analyses of the purified recombinant protein showed its ability to sense light by a red shift upon illumination. Therefore, the A1S_2225 gene, which is present in several members of the Acinetobacter genus, was named blue-light-sensing A (blsA). Interestingly, temperature plays a role in the ability of A. baumannii to sense and respond to light via the BlsA photoreceptor protein.

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Biofilm production by clinical strains ofAcinetobacter baumanniiisolated from patients hospitalized in two tertiary care hospitals

Cited by 43 publications

References 13 publications

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Acinetobacter infection – an emerging threat to human health

The Opportunistic Human PathogenAcinetobacter baumanniiSenses and Responds to Light

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