Biofilm Producing Staphylococcus epidermidis Strains Isolated From Clinical Samples in Tehran, Iran

Rahimi, Forough; Karimi, Saeed

doi:10.5812/archcid.33343

Cited by 6 publications

(8 citation statements)

References 37 publications

(53 reference statements)

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“…Observed results happen to be the same as Delpozo and Patel Filho's findings [8,17]. In 2016, two different studies investigated clinical isolates of S. epidermidis collected from a private hospital in Tehran [18] and clinical isolates from Shiraz city [19]. Zalipour reported that in 81.9% of clinical isolates of S. epidermidis, ica Luci was observed [19].…”

Section: Discussionsupporting

confidence: 74%

“…Zalipour reported that in 81.9% of clinical isolates of S. epidermidis, ica Luci was observed [19]. Rahimi reported that 82% of the isolated strains were biofilm-formers and all of the biofilm former S. epidermidis isolates carried icaA, icaD, aap, and atlE genes [18]. Granting to the previous findings, [20] sdrG and certain genes have a crucial role in biofilm formation of clinically isolated strains.…”

Section: Discussionmentioning

confidence: 70%

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Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

et al. 2020

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Objectives: Staphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible genes in biofilm formation procedure in clinical isolates (icaA, icaB, icaC, icaD, sdrG, and atlE) were assigned as the main objectives in this study. The clinical samples were analyzed via standard biochemical assays for identifying different bacteria which were confirmed using the multiplex colony PCR method. Subsequently, biofilm-formation capability, antibiotic susceptibility testing, and the frequency of genes responsible for biofilm formation in the confirmed strains were checked. Results: Out of 183 clinical specimens 54 S. epidermidis isolates were detected by targeting a housekeeping gene (sesc) taking advantage of the PCR procedure. All of the strains were Biofilm forming producers. The in vitro biofilm formation assays determined that 45 (83.33%), 5 (9.26%), 4 (7.41%) were strong, moderate, and weak biofilm former strains respectively. Among the isolated strains, the specific frequencies of the biofilm-forming genes were specified to be (98%) for sdrG, (84%) for atlE, (80%) for icaC, and (70%) for icaD. Cefamandole and Amikacin are the most effective antibiotics in isolated strains. All strains were ascertained to be methicillin and amoxicillin/clavulanic acid resistant.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 70%

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

et al. 2020

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“…Using a high-quality PFGE typing method, we found the presence of certain clonal groups of MRSE in patients with UTI at a referral hospital in Tehran, Iran. The MRSE strains in this study had the lowest frequency among hospitalized patients in Iran (12,20) that could be partly due to the fact that in this study, we focused on isolating MRSE strains from UTI patients with indwelling medical devices. The combination of two different quantitative and qualitative biofilm assays revealed that 89% of the MRSE strains were slime producers.…”

Section: Discussionmentioning

confidence: 93%

“…In this study, CTs 2 -4 and CT 6 were common among biofilm and non-biofilm producing isolates and none of the biofilm producing strains were present in CT 9. IS256 was found among all strains in 6 CTs (3,7,10,(12)(13)(14) while none of the strains in CTs 5 and 9 harbored this transposon.…”

Section: Typing Of Isolates By Pulsed-field Gel Electrophoresismentioning

confidence: 91%

“…A crystal violet assay was performed to measure the ability of the bacteria to adhere and form biofilms in trypticase soy broth (TSB) medium (Merck KGaA, Darmstadt, Germany) supplemented with sucrose, as described previously (12). The cutoff OD value (ODc) was defined as three standard deviations above the mean OD of the negative control.…”

Section: Quantitative Biofilm Assay Using a Microtiter Platementioning

confidence: 99%

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Molecular Characteristics of Biofilm-Producing Methicillin-Resistant Staphylococcus epidermidis Isolates Causing Urinary Tract Infections

Rahimi

2018

Arch Clin Infect Dis

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Background: Coagulase-negative Staphylococcus epidermidis is the most prevalent member of the human skin normal biota that plays an important role as a common cause of catheter and prosthetic device-related infections from, for example, indwelling catheters.Objectives: This study aimed to characterize the clonality of biofilm-producing methicillin-resistant S. epidermidis (MRSE) strains isolated from catheterized patients with urinary tract infection at a referral hospital in Tehran, Iran. Methods: Between 2014 and 2016, a total of 56 methicillin-resistant S. epidermidis (MRSE) strains were recovered from catheterized patients. The MRSE isolates were tested for biofilm formation and different genes involved in this process were detected. Clonal dissemination of MRSE isolates was determined using the combination of pulsed-field gel electrophoresis (PFGE) and SCCmec typing. Results: Out of the 56 MRSE strains, 50 (89%) formed biofilm and were positive for icaA and icaD genes, and 73% (n = 41) harbored IS256. The PFGE patterns revealed a total of 32 different pulsotypes consisting of 16 single types (STs), 16 common types (CTs), and 2 SCCmec types (III and IV) were detected. Moreover, all STs carried SCCmec type IV and classified as community-acquired strains. Four CTs were common among biofilm and non-biofilm producing strains. Conclusions:The presence of icaA and icaD genes with a high prevalence of IS256 element in clonal groups of MRSE strains suggests that ica, IS256, and biofilm forming ability occur simultaneously in specific S. epidermidis clones and spread preferentially in hospitals and community.

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Prevalence and distribution of resistance and enterotoxins/enterotoxin-like genes in different clinical isolates of coagulase-negative Staphylococcus

et al. 2020

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Background Coagulase-negative staphylococcus (CoNS) is considered to be the major reservoirs for genes facilitating the evolution of S. aureus as a successful pathogen. The present study aimed to determine the occurrence of genes conferring resistance to fluoroquinolone, determining of the prevalence of insertion sequence elements IS256, IS257 and different superantigens (SAgs) among CoNS isolates obtained from various clinical sources. Materials and methods The current study conducted on a total of the 91 CoNS species recovered from clinical specimens in Hamadan hospitals in western Iran in 2017–2019. The antimicrobial susceptibility testing was performed using disk diffusion method and the presence of the IS256 and IS257, genes conferring resistance to fluoroquinolone and enterotoxins/enterotoxin-like encoding genes were investigated by polymerase chain reaction (PCR) method. Results Among genes encoding classic enterotoxins, sec was the most frequent which was carried by 48.4% of the 91 isolates, followed by seb in 27.5% of the isolates. None of the CoNS isolates was found to be positive to enterotoxin-like encoding genes. In 11(12%) of all isolates that were phenotypically resistant to levofloxacin, 9 isolates (81.8%) were positive for gyrB, 8 isolates (72.7%) were positive for gyrA, 8 isolates (72.7%) harbored grlB and 7 isolates (63.6%) were found to carry grlA. The IS256 and IS257 were identified in 31.8% and 74.7% of the isolates, respectively. The results of statistical analysis showed a significant association between the occurrence of staphylococcal enterotoxins (SEs) encoding genes and antimicrobial resistance. Conclusion Antimicrobial resistant determinants and SEs are co-present in clinical CoNS isolates that confer selective advantage for colonization and survival in hospital settings. The coexistence of insertion elements and antibiotic resistance indicate their role in pathogenesis and infectious diseases.

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Biofilm Producing Staphylococcus epidermidis Strains Isolated From Clinical Samples in Tehran, Iran

Abstract: Background: Staphylococcus epidermidis is well documented as an opportunistic pathogen causing biofilm in patients and healthy individuals.

Cited by 6 publications

References 37 publications

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

Molecular Characteristics of Biofilm-Producing Methicillin-Resistant Staphylococcus epidermidis Isolates Causing Urinary Tract Infections

Prevalence and distribution of resistance and enterotoxins/enterotoxin-like genes in different clinical isolates of coagulase-negative Staphylococcus

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