Biofilm-producing ability of Listeria monocytogenes isolates from Brazilian cheese processing plants

Lee, Sarah Hwa In; Barancelli, Giovana Verginia; Camargo, Tarsila Mendes de; Corassin, Carlos Humberto; Rosim, Roice Eliana; Cruz, Adriano G.; Cappato, Leandro P.; Oliveira, Carlos Augusto Fernandes de

doi:10.1016/j.foodres.2016.11.039

Cited by 47 publications

(16 citation statements)

References 37 publications

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“…-weak (OD NC < OD ≤ 2xOD NC ); -moderate (2xOD NC < OD ≤ 4xOD NC ); -strong (4xOD NC < OD); -no biofilm formation (OD ≤ OD NC ), according to the description Lee et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri

Knysh

2019

Regul. Mech. Biosyst.

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Comprehensive study of the biological activity of structural components and metabolites of “beneficial” microorganisms opens the prospects of efficient and rational use of their biotechnological potential in the correction of microecological and related disorders. The study tested proliferative activity and biofilm formation by Bifidobacterium bifidum probiotic strain under the influence of cell-free extracts containing structural components and metabolites of the probiotic strains of B. bifidum and Lactobacillus reuteri. Cell-free extracts were obtained by disintegrating suspensions of probiotic cells by cyclic freezing-thawing, cultivating probiotic microorganisms in their own disintegrates and subsequent filtration of the obtained disintegrates and cultures. The proliferative activity and biofilm formation of the probiotic test culture were studied by spectrophotometric microtiter plate method with 10%vol, 30%vol and 50%vol content of cell-free extracts in the cultivation medium. All investigated extracts showed a significant concentration-dependent stimulatory effect on the proliferative activity of B. bifidum. According to the degree of stimulatory effect on the B. bifidum proliferation, cell-free extracts arranged in ascending order: MLG (filtrate of L. reuteri culture, grown in L. reuteri disintegrate supplemented with 0.8 M glycerol and 0.4 M glucose) < MB (filtrate of В. bifidum culture, grown in В. bifidum disintegrate) < B (filtrate of В. bifidum disintegrate) < ML (filtrate of L. reuteri culture, grown in L. reuteri disintegrate) < L (filtrate of L. reuteri disintegrate). With the same content in the culture medium, filtrates of disintegrates had a more pronounced stimulatory effect than filtrates of cultures grown in their own disintegrates. Cell-free extracts from L. reuteri (L and ML) exerted a more pronounced stimulatory effect than cell-free extracts from B. bifidum. Not all studied cell-free extracts stimulated the biofilm formation by B. bifidum. The effect of cell-free extracts on this process depended on their type and concentration. Extract L had a predominantly inhibitory effect on biofilm formation by B. bifidum. The most pronounced stimulatory effect on biofilm formation by B. bifidum came from extract MLG. ML, B and MB extracts stimulated this process approximately equally. The detection of significant bifidogenic effect of the studied cell-free extracts may contribute to their pharmaceutical applications. Cell-free extracts can be used as metabiotics or prebiotics for increasing the survival of the injected probiotic, facilitating its inoculation in the gastrointestinal tract when used together. The obtained data encourage further careful study of the biochemical composition of cell-free extracts and efforts to clarify the mechanism of their action.

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“…-weak (OD NC < OD ≤ 2xOD NC ); -moderate (2xOD NC < OD ≤ 4xOD NC ); -strong (4xOD NC < OD); -no biofilm formation (OD ≤ OD NC ), according to the description Lee et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri

Knysh

2019

Regul. Mech. Biosyst.

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“…-weak (OD NC < OD ≤ 2xOD NC ); -moderate (2xOD NC < OD ≤ 4xOD NC ); -strong (4xOD NC < OD); -no biofilm formation (OD ≤ OD NC ), according to the method for quantification of biofilm formation proposed by In Lee S. et al [16].…”

Section: Methodsmentioning

confidence: 99%

The effects of cell-free extracts derived from probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri on the proliferation and biofilm formation by Lactobacillus reuteri in vitro

Knysh

2019

ZMJ

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The aim of the research was to investigate the ability of cell-free extracts, containing derivatives of probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri, to influence the proliferation and biofilm formation by Lactobacillus reuteri in vitro; to evaluate the prospects for the creation of new metabiotics and means of increasing the overall productivity of probiotic cell biomass based on them.Materials and methods. Cell-free extracts were obtained from probiotic strains B. bifidum 1 and L. reuteri DSM 17938 by the authors' method. Probiotic strain L. reuteri DSM 17938 used as a test culture. The investigation of the proliferation and biofilm formation by L. reuteri was carried out by spectrophotometric method using a microtiter-plate reader "Lisa Scan EM" (Erba Lachema s.r.o., Czech Republic). Results.It has been established that the cell-free extract obtained from L. reuteri culture grown in its own disintegrate, supplemented with glycerol and glucose, is the only one of all studied, which stimulates both proliferation and biofilm formation by L. reuteri. The cell-free extracts, obtained from L. reuteri and B. bifidum disintegrates and from L. reuteri culture, grown in its own disintegrate, stimulate proliferation of test-culture to varying degrees (depending on the type of extract and its content in the culture medium), but have a significant inhibitory effect on the biofilm formation by L. reuteri. The extract, obtained from B. bifidum culture, grown in its own disintegrate, does not have a significant effect on proliferation and greatly suppresses the biofilm formation by L. reuteri. Conclusions.The results of the study allow us to positively evaluate the prospects for the creation of new metabiotics based on probiotic derivatives. Cell-free extract, obtained from L. reuteri culture, grown in its own disintegrate supplemented with glycerol and glucose can contribute to the survival and facilitate inoculation of the introduced probiotic in the gastrointestinal tract when used together.Derivative-containing extracts with a pronounced growth-stimulating effect can be the basis for creating the means of increasing the overall productivity of the probiotic cell biomass. The use of such means will increase the economic efficiency of probiotic cultures production. Obtained data induce further study of the biochemical composition, elucidation of the mechanism of cellfree extracts action and confirmation of their efficacy in vivo. Вплив безклітинних екстрактів, що отримані з пробіотичних штамів Bifidobacterium bifidum та Lаctobacillus reuteri, на проліферацію та біоплівкоутворення Lactobacillus reuteri in vitro О. В. Книш Мета роботи -дослідити здатність безклітинних екстрактів, що містять деривати пробіотичних штамів Bifidobacterium bifidum і Lactobacillus reuteri, впливати на проліферацію та біоплівкоутворення Lactobacillus reuteri in vitro; оцінити перспективи створення на їхній основі нових метабіотиків, засобів підвищення загальної продуктивності клітинної біомаси пробіотика. Матеріали та методи. ...

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“…Biofilms are considered as highly organised forms, allowing the exchange of nutrients and metabolics in the same ecosystem in order to guarantee survival (Shiand Zhu, 2009). Among foodborne microorganisms that form biofilms on surfaces (Figure 1), Staphylococcus aureus (Lee et al, 2014), Bacillus cereus (Ehling-Schulz et al, 2019;Gopal et al, 2015), Listeria monocytogenes (Lee et al, 2017a), Escherichia coli (Cherif-Antar et al, 2016), Salmonella spp. (Wang et al, 2016) and Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

“…L. monocytogenes is another pathogenic bacterium whose biofilms represent a significant problem in dairy processing areas, mainly because of the contamination of food handling surfaces, equipment and pipes (Kadam et al, 2013). Formation of biofilms of L. monocytogenes depends on several factors, such as pH, surface material for adhesion, availability of inorganic or organic matter, resistance to adhesion and temperature (Lee et al, 2017a(Lee et al, , 2017b. According to Silva and De Martinis (2013), L. monocytogenes can adhere and form biofilms at a cell concentration of 10 4 -10 7 UFC/cm 2 .…”

Section: Introductionmentioning

confidence: 99%

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Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

Gonçalves

Lee

Khaneghah

et al. 2020

qas

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In this review, formation of biofilms and the available data on enzyme-based preparations to control microbial biofilms in dairy processing environments are presented. Mature biofilms, especially those formed by pathogenic bacteria, have increased resistance to biocides, hence stressing the importance of stringent hygienic procedures. Proteases, amylases, cellulases and DNAses are among the most recently studied enzymes that could be associated with the main biocides used in the dairy industry to increase the effect of removal of biofilm. However, addi-tional studies should be conducted to select the best cost-benefit combinations of selected enzymes and biocides to remove efficiently biofilms in dairy processing environments.

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Biofilm-producing ability of Listeria monocytogenes isolates from Brazilian cheese processing plants

Cited by 47 publications

References 37 publications

Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri

Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri

The effects of cell-free extracts derived from probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri on the proliferation and biofilm formation by Lactobacillus reuteri in vitro

Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

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