Biofilm prevention by dicephalic cationic surfactants and their interactions with DNA

Piecuch, Agata; Lamch, Łukasz; Paluch, Emil; Obłąk, Ewa; Wilk, Kazimiera A.

doi:10.1111/jam.13204

Cited by 21 publications

(16 citation statements)

References 54 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, the dicephalic QASs were tested for adhesion reduction on the surface of polystyrene, silicone, stainless steel and glass. As in current research, the inhibition of S. epidermidis adhesion was not observed on the surface of polystyrene but was significant on the hydrophilic surface of stainless steel (Piecuch et al ). Also dimeric (gemini) cationic surfactants are reported to possess strong antiadhesive properties.…”

Section: Resultssupporting

confidence: 57%

“…The correlation between hydrocarbon chain length and biological activity was demonstrated for other types of surfactants, including dicephalic QASs and dihydroxy quaternary ammonium salts. The biological activity of tested dicephalic quaternary ammonium bromides was the highest for C 14 and C 16 compounds but regarding dihydroxy quaternary ammonium bromide (single‐head) the MICs were the lowest for C 12 (Liu et al ; Piecuch et al ). Generally in homologous series of QASs the antibacterial effect rises with the elongation of the hydrophobic chain and it is the highest for C 12 ‐C 16 .…”

Section: Resultsmentioning

confidence: 99%

“…Not only conventional QASs (monomeric) but also more complex cationic surfactants may have antimicrobial properties. It was suggested that dimeric QASs (gemini) could be used as disinfectant agents because of their strong activity that depends mainly on the type of substituents, alkyl chain length,type and length of the linker (Piecuch et al ; Brycki et al ). Moreover, it was proved that dicephalic surfactants exhibit high, structure‐dependent antimicrobial activity, inhibiting the growth of bacteria and yeast (Piecuch et al ; Paluch et al ).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Biological activity of glycine and alanine derivatives of quaternary ammonium salts (QASs) against micro‐organisms

Rewak‐Soroczyńska

Paluch

Siebert

et al. 2019

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

Quaternary ammonium salts (QASs) are commonly used in medicine, agriculture and industry and their wide usage caused the development of microbial resistance, thus there is still a need for new effective antimicrobial agents. Present work describes the biological activity of alanine‐ (DMALM‐n) and glycine‐derived (DMGM‐n) QASs against planktonic and biofilm forms of micro‐organisms. The antimicrobial activity was dependent mainly on the hydrocarbon chain length and surfactants with 12–16 atoms of carbon in the alkyl chain were the most active ones. The lowest MIC value was determined for DMALM‐14 against Rhodotorula rubra and Saccharomyces cerevisiae (2·5 µmol l−1). Generally, alanine derivatives showed stronger effects against micro‐organisms than glycine‐derived QASs. Alanine‐derived surfactants with 12–16 carbons in the alkyl chain had antiadhesive properties on the polystyrene surface, preventing cell attachment (about 70% of inhibition for C. albicans and 40% for S. epidermidis). Strong adhesion reduction was also observed on the stainless steel surface and the highest reduction was observed for C. albicans cells incubated on surface pretreated with DMGM‐16. Moreover, DMGM‐16 and DMALM‐16 prevented C. albicans filamentation, one of the determinants of cell adhesion. Surfactants with C16 alkyl chain (DMGM‐16 and DMALM‐16) eradicated bacterial and yeast biofilm (from 60 to 90% of reduction observed after incubation of the previously grown biofilm in the presence of the highest tested concentration of the surfactant – 400 µmol l−1) and reduced its viability. Strong antimicrobial activity as well as antiadhesive properties make alanine‐ and glycine‐derived QASs the potential candidates for future application as disinfectants. Significance and Impact of the Study Cationic surfactants are used in many fields, among others in medicine, cosmetic and pharmaceutical industry. Their usage on a large scale caused the development of microbial resistance mechanisms to such compounds. Thus, there is a need to synthesize new surfactants with potential application as effective disinfectants to combat both planktonic and biofilm forms of micro‐organisms. Present work focuses on the antimicrobial activity of chosen quaternary ammonium salts.

show abstract

Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Biological activity of glycine and alanine derivatives of quaternary ammonium salts (QASs) against micro‐organisms

Rewak‐Soroczyńska

Paluch

Siebert

et al. 2019

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…That may be obtained by coating surfaces with antimicrobial agents such as metal-based nanoparticles, surfactants (e.g., quaternary ammonium salts (QAS)), or others. Adhesion reduction was observed for S. epidermidis and C. albicans cells incubated on pre-treated with dicephalic QAS glass, stainless steel, and silicone surfaces; thus, such compounds may be used to produce resistant to bacterial adhesion medical tools (e.g., catheters) what can lower a risk of nosocomial infections (Paluch et al 2018;Piecuch et al 2016). Moreover such compounds are able to decrease the ability to bacterial biofilm production on different metal surfaces, so they may be applied as anti-corrosive and anti-biofilm products (e.g., paints) to protect objects (such as ships, pipes) from degradation (Piecuch et al 2016;Paluch et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

Self Cite

270

151

View full text Add to dashboard Cite

Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

show abstract

“…The coating of titanium with PEG was shown to reduce the adhesion of Streptococcus sanguinis and Lactobacillus salivarius as well as fibroblast adhesion . Cationic dicephalic surfactants differing in hydrocarbon chain length were shown to have an antibacterial effect on planktonic Staphylococcus epidermidis and cause a reduction in its biofilm formation on glass and stainless steel . Pluronic F127 (Poloxamer 407) was reported to resist multiple washes when impregnated into a hydrophobic surface and subsequently could reduce S. epidermidis attachment and biofilm formation …”

Section: Biofilms and Surfactantsmentioning

confidence: 99%

Mode of action of poloxamer‐based surfactants in wound care and efficacy on biofilms

Percival¹,

Chen²,

Mayer

et al. 2018

International Wound Journal

View full text Add to dashboard Cite

Surfactants are widely used as detergents, emulsifiers, wetting agents, foaming agents, and dispersants in both the food and oil industry. Their use in a clinical setting is also common, particularly in wound care. Complicated or chronic wounds show clinical signs of delayed healing, persistent inflammation, and the production of non-viable tissue. These types of wounds also present challenges such as infection and potentially house antimicrobial-tolerant biofilms. The use of wound cleansers to aid cleaning and debridement of the wound is essential. A large proportion of skin and wound cleansers contain surfactants but there is only a small amount of data that shows the effectiveness of them in the enhancement of wound closure. This review paper aims to explore the available literature surrounding the use and mode of action of surfactants in wound healing, in particular Poloxamer 188 (Pluronic F-68) and Poloxamer 407 (Pluronic F-127), and also uncover the potential mechanisms behind the enhancement of wound healing and comparison to other surfactants used in wound care. Furthermore, the presence of a microbial biofilm in the wound is a significant factor in delayed wound healing. Therefore, the effect of clinically used surfactants on biofilms will be discussed, with emphasis on poloxamer-based surfactants.

show abstract

Biofilm prevention by dicephalic cationic surfactants and their interactions with DNA

Abstract: This study points the possibility of application of dicephalic surfactants as the surface-coating agents to prevent biofilm formation. These compounds efficiently condensed DNA and are potential candidates for further studies towards the transfection.

Cited by 21 publications

References 54 publications

Biological activity of glycine and alanine derivatives of quaternary ammonium salts (QASs) against micro‐organisms

Biological activity of glycine and alanine derivatives of quaternary ammonium salts (QASs) against micro‐organisms

Prevention of biofilm formation by quorum quenching

Mode of action of poloxamer‐based surfactants in wound care and efficacy on biofilms

Contact Info

Product

Resources

About