Biofilm modulatory response of arginine-fluoride varnish on multi-species biofilm

Bijle, Mohammed Nadeem Ahmed; Ashraf, Usman; Abdalla, Mohamed Mahmoud; Neelakantan, Prasanna; Yiu, Cynthia Kar Yung

doi:10.1016/j.jdent.2022.104096

Cited by 10 publications

(3 citation statements)

References 38 publications

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“…Although the objective of this work was to evaluate the antimicrobial activity and cytotoxicity of ibuprofen and diclofenac solutions against an endodontic biofilm, we considered it appropriate to include a solution of ibuprofen combined with arginine given its efficacy against different oral biofilms [ 31 , 32 ], as well as the proven synergistic effects when combined with antibiotics in antimicrobial complexes [ 36 , 37 ]. Still, in view of the results obtained here, further investigations are needed, including arginine solutions alone and at different concentrations, to assess their true potential against polymicrobial root canal biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…The antibiofilm effects of arginine on mono-species [ 31 ] and multi-species cariogenic biofilm [ 32 ] are related to polysaccharide reduction and the prebiotic concentration used. To date, however, its antimicrobial activity against polymicrobial biofilms obtained from necrotic root canals of teeth with apical periodontitis is unknown.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Ferrer-Luque

Solana

Aguado³

et al. 2023

Antibiotics

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Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Ferrer-Luque

Solana

Aguado³

et al. 2023

Antibiotics

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“…S. mutans biofilms were incubated on confocal dishes with or without the addition of nanogels at concentrations of 25-200 mg/mL and rinsed with PBS to remove floating bacteria. The biofilms were then stained with 2.5 μM SYTO 9 and propidium iodide (Invitrogen, USA) as described previously 24 and observed by CLSM with a 40× lens objective (Olympus FV3000, Japan), and the percentages of live and dead cells were quantified using COMSTAT image-processing software.…”

mentioning

confidence: 99%

Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

Yuan,

Zhang,

Liao

et al. 2023

IJN

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Background: Dentin caries remains a significant public concern, with no clinically viable material that effectively combines remineralization and antimicrobial properties. To address this issue, this study focused on the development of a bio-inspired multifunctional nanogel with both antibacterial and biomineralization properties. Methods: First, p(NIPAm-co-DMC) (PNPDC) copolymers were synthesized from N-isopropylacrylamide (NIPAm) and 2-methacryloyloxyethyl-trimethyl ammonium chloride (DMC). Subsequently, PNPDC was combined with γ-polyglutamic acid (γ-PGA) through physical cross-linking to form nanogels. These nanogels served as templates for the mineralization of calcium phosphate (Cap), resulting in Cap-loaded PNPDC/PGA nanogels. The nanogels were characterized using various techniques, including TEM, particle tracking analysis, XRD, and FTIR. The release properties of ions were also assessed. In addition, the antibacterial properties of the Cap-loaded PNPDC/PGA nanogels were evaluated using the broth microdilution method and a biofilm formation assay. The remineralization effects were examined on both demineralized dentin and type I collagen in vitro. Results: PNPDC/PGA nanogels were successfully synthesized and loaded with Cap. The diameter of the Cap-loaded PNPDC/PGA nanogels was measured as 196.5 nm at 25°C and 162.3 nm at 37°C. These Cap-loaded nanogels released Ca 2+ and PO 4 3ions quickly, effectively blocking dental tubules with a depth of 10 μm and promoting the remineralization of demineralized dentin within 7 days. Additionally, they facilitated the heavy intrafibrillar mineralization of type I collagen within 3 days. Moreover, the Cap-loaded nanogels exhibited MIC 50 and MIC 90 values of 12.5 and 50 mg/mL against Streptococcus mutans, respectively, with an MBC value of 100 mg/mL. At a concentration of 50 mg/mL, the Cap-loaded nanogels also demonstrated potent inhibitory effects on biofilm formation by Streptococcus mutans while maintaining good biocompatibility. Conclusion:Cap-loaded PNPDC/PGA nanogels are a multifunctional biomimetic system with antibacterial and dentin remineralization effects. This strategy of using antibacterial nanogels as mineral feedstock carriers offered fresh insight into the clinical management of caries.

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Effects of caffeic acid phenethyl ester against multi-species cariogenic biofilms

Lv¹,

Feng²,

Luo³

et al. 2023

Folia Microbiol

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Biofilm modulatory response of arginine-fluoride varnish on multi-species biofilm

Cited by 10 publications

References 38 publications

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

Effects of caffeic acid phenethyl ester against multi-species cariogenic biofilms

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