Biofilm Models to Study the Etiology and Pathogenesis of Oral Diseases

Thurnheer, Thomas; Paqué, Pune Nina

doi:10.1159/000510197

Cited by 9 publications

(8 citation statements)

References 36 publications

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“…This well-established ecological succession model describes that early colonizing species first bind to the pellicle and form the basis for subsequent attachment of intermediate and late colonizers ( 7 ). In vitro systems using pure cultures of selected bacterial species in a planktonic state have been used with or without saliva, providing support for the sequential biofilm community assembly concept ( 16 , 32 ). While such interactions have been demonstrated experimentally, we propose that, in addition to single-cell events, natural aggregated mixed communities can also colonize tooth surfaces in active form, and these preformed consortia have advantages over the single-cell counterparts under our experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm

Simón-Soro

Ren

Krom

et al. 2022

mBio

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Section: Discussionmentioning

confidence: 99%

Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm

Simón-Soro

Ren

Krom

et al. 2022

mBio

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“…While an S. aureus monoculture was used in Test series I, a heterogeneous (thus natural and complex) biofilm was used in Test series II. As described in literature, the monoculture is an artificial circumstance, as microorganisms normally grow in complex ecological systems in natural habitats [ 54 ]. It is possible that as the complexity of the biofilm increases, the laser radiation can penetrate less deeply into the biofilm structure.…”

Section: Discussionmentioning

confidence: 99%

Effect of Er,Cr:YSGG laser with a side-firing tip on decontamination of titanium disc surface: an in vitro and in vivo study

et al. 2023

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Purpose To evaluate the effectiveness of an erbium, chromium:yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser with side-firing tip in decontamination of titanium (Ti) disc. Methods In the first test series, 29 Ti-discs were contaminated with Staphylococcus aureus and treated as follows: positive control (no treatment); Perioflow; Laser A (0.75 W, 100 Hz), Laser B (1.5 W, 30 Hz); Laser C (no radiation, 60% water); and Laser D (no radiation, 50% water). For bacterial quantification, colony forming units (CFU, vital cells only) and quantitative PCR (qPCR, vital and devital cells) were performed. In a second test series, 92 Ti-discs were used, contaminated with in vivo-grown biofilm and treated as follows: positive control (no treatment); Perioflow; Laser E (1.5 W, 30 Hz), and Laser F (no radiation, 50% water). Considering the different and unknown culture conditions, quantification of bacteria was performed by broad-spectrum bacterial qPCR only. Based on the assumption that all cells of an organism contain an equivalent complement of genetic information, genome equivalent (GE) determination ensured the detection of the different intact and semi-intact genomes, regardless of type of bacterial species and vitality, circumvent the inherent bias of cultures. Results The GE values were significantly reduced by all interventions in both test series, compared to the positive control group (p < 0.001). In the first test series with S. aureus as model organism, Perioflow yielded a lower GE than the Laser groups A–D (all p < 0.025). The number of CFUs was significantly reduced in the intervention groups compared to the positive control (p < 0.001), except for Laser A (p = 0.157) and Laser D (p = 0.393). In the second test series, none of the pairwise comparisons of the intervention conditions showed a significant difference (Perioflow vs. Laser E: p = 0.732; Perioflow vs. Laser F: p = 0.590; Laser E vs. Laser F: p = 0.379). Conclusion The Er,Cr:YSGG laser with side-firing tip and Perioflow were equally capable of effectively decontaminating a Ti-disc surface. It is assumed that the bacterial reduction was largely due to the mechanical effect of the air and water stream. Graphical Abstract

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“…The etiology and pathogenesis of biofilms (plaque) are best studied in sophisticated in vitro models [16]. The virulent bacteria in plaque are primarily Streptococcus mutans (S. Mutans) as well as numerous Lactobacillus species that metabolize dietary sugar to produce lactic acid.…”

Section: Infectionsmentioning

confidence: 99%

Pathophysiology of Demineralization, Part II: Enamel White Spots, Cavitated Caries, and Bone Infection

Roberts

Mangum

Schneider

2022

Curr Osteoporos Rep

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Purpose of Review Compare noninfectious (part I) to infectious (part II) demineralization of bones and teeth. Evaluate similarities and differences in the expression of hard tissue degradation for the two most common chronic demineralization diseases: osteoporosis and dental caries. Recent Findings The physiology of demineralization is similar for the sterile skeleton compared to the septic dentition. Superimposing the pathologic variable of infection reveals a unique pathophysiology for dental caries. Summary Mineralized tissues are compromised by microdamage, demineralization, and infection. Osseous tissues remodel (turnover) to maintain structural integrity, but the heavily loaded dentition does not turnover so it is ultimately at risk of collapse. A carious tooth is a potential vector for periapical infection that may be life-threatening. Insipient caries is initiated as a subsurface decalcification in enamel that is not detectable until a depth of ~400μm when it becomes visible as a white spot. Reliable detection and remineralization of invisible caries would advance cost-effective wellness worldwide.

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Biofilm Models to Study the Etiology and Pathogenesis of Oral Diseases

Cited by 9 publications

References 36 publications

Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm

Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm

Effect of Er,Cr:YSGG laser with a side-firing tip on decontamination of titanium disc surface: an in vitro and in vivo study

Pathophysiology of Demineralization, Part II: Enamel White Spots, Cavitated Caries, and Bone Infection

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