Biofilm-Mediated Heavy Metal Removal from Aqueous System by Multi-Metal-Resistant Bacterial Strain Bacillus sp. GH-s29

Maity, Sourav; Sarkar, Debapriya; Poddar, Kasturi; Patil, Pritam Bajirao; Sarkar, Angana

doi:10.1007/s12010-022-04288-7

Cited by 13 publications

(7 citation statements)

References 56 publications

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“…A quantitative proteomic analysis carried out to study the mechanisms of tolerance and resistance to arsenite, in the presence or absence of cyanide, has revealed that in this strain the mechanisms of resistance to arsenic include the extrusion of arsenite through the ArsAB efflux pump and the production of arsenical derivatives, such as arsenophosphoglycerate formed by an arsenic inducible glyceraldehyde-3-phosphate dehydrogenase, or methylarsenite formed by ArsM-type methyltransferases, which are further exported through ArsJ, ArsP and Acr3 permeases. Extruded arsenite may be accumulated in the bacterial biofilm , as it has been also recently reported in other microorganisms (Barral-Fraga et al, 2018;Maity et al, 2022;Mathivanan et al, 2021) (Figure 3). In the proteomic analysis of P. pseudoalcaligenes CECT 5344 it was also observed that not only the response to arsenite was different depending on the nitrogen source, ammonium or cyanide, but also that the presence of cyanide or arsenite, independently, induces proteins related to oxidative stress, biofilm formation, cyanide metabolism and arsenic metabolism .…”

Section: Bacterial Arsenic Detoxificationsupporting

confidence: 74%

Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

Olaya‐Abril,

Biełło,

Rodríguez‐Caballero

et al. 2024

Microbial Biotechnology

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Cyanide is a highly toxic compound that is found in wastewaters generated from different industrial activities, such as mining or jewellery. These residues usually contain high concentrations of other toxic pollutants like arsenic and heavy metals that may form different complexes with cyanide. To develop bioremediation strategies, it is necessary to know the metabolic processes involved in the tolerance and detoxification of these pollutants, but most of the current studies are focused on the characterization of the microbial responses to each one of these environmental hazards individually, and the effect of co‐contaminated wastes on microbial metabolism has been hardly addressed. This work summarizes the main strategies developed by bacteria to alleviate the effects of cyanide, arsenic and heavy metals, analysing interactions among these toxic chemicals. Additionally, it is discussed the role of systems biology and synthetic biology as tools for the development of bioremediation strategies of complex industrial wastes and co‐contaminated sites, emphasizing the importance and progress derived from meta‐omic studies.

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Section: Bacterial Arsenic Detoxificationsupporting

confidence: 74%

Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

Olaya‐Abril,

Biełło,

Rodríguez‐Caballero

et al. 2024

Microbial Biotechnology

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“…It has been recently reported that As may be retained by the bacterial biofilm [ 17 , 55 , 60 ]. In addition, some proteins such as PilJ (BN5_0312), RfbA (BN5_4136), and RfbC (BN5_4137), which are involved in biofilm formation [ 52 , 61 , 62 ] were found to be up-regulated by arsenite in the proteomic analysis ( Table 1 , SDS2).…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Biełło

Cabello

Rodríguez-Caballero

et al. 2023

IJMS

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Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the simultaneous presence of cyanide and arsenite were analyzed by quantitative proteomics, complemented with qRT-PCR analysis and determination of analytes in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes CECT 5344. Several proteins encoded by two ars gene clusters and other Ars-related proteins were up-regulated by arsenite, even during cyanide assimilation. Although some proteins encoded by the cio gene cluster responsible for cyanide-insensitive respiration decreased in the presence of arsenite, the nitrilase NitC required for cyanide assimilation was unaffected, thus allowing bacterial growth with cyanide and arsenic. Two complementary As-resistance mechanisms were developed in this bacterium, the extrusion of As(III) and its extracellular sequestration in biofilm, whose synthesis increased in the presence of arsenite, and the formation of organoarsenicals such as arseno-phosphoglycerate and methyl-As. Tetrahydrofolate metabolism was also stimulated by arsenite. In addition, the ArsH2 protein increased in the presence of arsenite or cyanide, suggesting its role in the protection from oxidative stress caused by both toxics. These results could be useful for the development of bioremediation strategies for industrial wastes co-contaminated with cyanide and arsenic.

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“…For instance, the metal concentration within the LRS1 strain biomass was recorded at 9.23 mg/g. This discrepancy could be attributed to this discrepancy, including adsorption of metals onto the bacterial cell surface or extracellular precipitation ( Maity et al, 2023 ). SEM result provided visual evidence supporting these mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

El-Beltagi,

Halema,

Almutairi

et al. 2024

Front. Bioeng. Biotechnol.

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Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance genes were evaluated. Our findings revealed a statistically significant upregulation in gene expression under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals. The outstanding potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion makes it a valuable candidate for developing safe and effective strategies for heavy metal disposal.

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Biofilm-Mediated Heavy Metal Removal from Aqueous System by Multi-Metal-Resistant Bacterial Strain Bacillus sp. GH-s29

Cited by 13 publications

References 56 publications

Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

Bacterial tolerance and detoxification of cyanide, arsenic and heavy metals: Holistic approaches applied to bioremediation of industrial complex wastes

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

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