Biofilm mediated decontamination of pollutants from the environment

Mitra, Arindam; Mukhopadhyay, Suman

doi:10.3934/bioeng.2016.1.44

Cited by 73 publications

(32 citation statements)

References 104 publications

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“…However, most of these methods are expensive, ineffective, and required high energy and produced large amount of sludge with hazardous by-products ( Ahluwalia and Goyal, 2007 ; Dixit et al, 2015 ). By contrast, microbial-based techniques are eco-friendly, economic, and effectively detoxify the persistent organic pollutants (POPs), petroleum products, explosives, dyes, and metals from wastewater ( Singh et al, 2006 ; Saratale et al, 2011 ; Edwards and Kjellerup, 2013 ; Elekwachi et al, 2014 ; Dixit et al, 2015 ; Mitra and Mukhopadhyay, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

et al. 2018

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Bacterial biofilm plays a pivotal role in bioremediation of heavy metals from wastewaters. In this study, we isolated and identified different biofilm producing bacteria from wastewaters. We also characterized the biofilm matrix [i.e., extracellular polymeric substances (EPS)] produced by different bacteria. Out of 40 isolates from different wastewaters, only 11 (27.5%) isolates (static condition at 28°C) and 9 (22.5%) isolates (agitate and static conditions at 28 and 37°C) produced air–liquid (AL) and solid–air–liquid (SAL) biofilms, respectively, only on salt-optimized broth plus 2% glycerol (SOBG) but not in other media tested. Biomass biofilms and bacteria coupled with AL biofilms were significantly (P ≤ 0.001) varied in these isolates. Escherichia coli (isolate ENSD101 and ENST501), Enterobacter asburiae (ENSD102), Enterobacter ludwigii (ENSH201), Pseudomonas fluorescens (ENSH202 and ENSG304), uncultured Vitreoscilla sp. (ENSG301 and ENSG305), Acinetobacter lwoffii (ENSG302), Klebsiella pneumoniae (ENSG303), and Bacillus thuringiensis (ENSW401) were identified based on 16S rRNA gene sequencing. Scanning electron microscope (SEM) images revealed that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, A. lwoffii ENSG302, and K. pneumoniae ENSG303 are highly fibrous, compact, and nicely interlinked as compared to the biofilm developed by E. ludwigii ENSH201 and B. thuringiensis ENSW401. X-ray diffraction (XRD) results indicated that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 are non-crystalline amorphous nature. Fourier transform infrared (FTIR) spectroscopy showed that proteins and polysaccharides are the main components of the biofilms. Congo red binding results suggested that all these bacteria produced proteinaceous curli fimbriae and cellulose-rich polysaccharide. Production of cellulose was also confirmed by Calcofluor binding- and spectrophotometric assays. E. asburiae ENSD102, Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 were tested for their abilities to form the biofilms exposure to 0 to 2000 mg/L of copper sulfate (for Cu), zinc sulfate (for Zn), lead nitrate (for Pb), nickel chloride (for Ni), and potassium dichromate (for Cr), several concentrations of these metals activated the biofilm formation. The polysaccharides is known to sequester the heavy metals thus, these bacteria might be applied to remove the heavy metals from wastewater.

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Section: Introductionmentioning

confidence: 99%

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

et al. 2018

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“…Application of genetically engineered bacteria (bioremediation) improved biodegradation of pollutants in environments (28). In this study, genetically engineered P. putida that produce dioxygenase enzyme was generated via transferring of pUC18-nahH recombinant vector and were used for biodegradation of oil in the spiked soil.…”

Section: Discussionmentioning

confidence: 99%

Application of Genetically Engineered Dioxygenase Producing Pseudomonas putida on Decomposition of Oil from Spiked Soil

Mardani

Mahvi

Hashemzadeh‐Chaleshtori

et al. 2017

Jundishapur J Nat Pharm Prod

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Background: It is well known that bioremediation or using microorganism enzymes plays an important role in decomposition and changing of oil pollutants and PAH compounds into nonhazardous or less-hazardous substances. The genetically manipulated bacteria like Pseudomonas sp. can enhancement the natural biodegradation activity of polycyclic aromatic hydrocarbons (PAHs) in polluted cities. Pseudomonas putida (P. putida) is a saprotrophic soil bacterium that is found throughout various environments such as soil and freshwater environments. Oil and petroleum industries are important exposure of PAHs in environment that strongly associated with the development of human cancers. Objectives: In the present work, P. putida was genetically manipulated for the biodegradation of oil in spiked soil and its activity was measured using the high-performance liquid chromatography (HPLC) method. Methods: The catechol 2,3-dioxygenase (C23O) encoded gene (nahH) was cloned into pUC18 for generating pUC18-nahH. This recombinant vector was transferred into P. putida, successfully, and both wild type and genetically modified P. putida were inoculated in spiked soil with oil for pilot plan preparation. Then, biodegradation activity of this bacterium on the elimination of phenanthrene and pyrene as an oil indicator in spiked soil were evaluated by the HPLC measurement technique. Results:The results showed the biodegradation of these PAH compounds in oil-spiked soil by genetically manipulated P. putida in both groups (containing autoclaved soil and dish containing natural soil) comparing to the inoculated group by wild type of P. putida were statistically significant (P < 0.05). Finally, confirmatory tests (catalase, oxidase, and PCR) were accomplished on isolated bacteria from spiked soil and were indicated that engineered P. putida was alive and functional by producing the C23O enzyme for biodegradation activity of oil. Conclusions: These findings demonstrated a degradable strain of P. putida that was generated in this study by genetic engineering can be useful to assess biodegradation of PAHs compounds in oil and petrochemical pollutions.

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“…One of the aim of this study was also to determine the biofilm formation abilities of the tested strains. Biofilm is composed of highly structured multispecies bacterial communities embedded in a self-produced polymeric matrix called extracellular polymeric substances (EPS) [20,21]. Biofilms as a mode of the bacterial growth predominate in a different ecosystems.…”

Section: Eko-dok 2017mentioning

confidence: 99%

Norfloxacin degradation byBacillus subtilisstrains able to produce biosurfactants on a bioreactor scale

2017

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Abstract. The discharge of antibiotics into the environment has become a major concern since this group of pharmaceuticals influence on microbial communities not only by its mode of action, but also because of the risk of a worldwide dispersal of antibiotic resistance genes (ARG). Antibiotics residues have been found in various environments such as waters, sediments, and soils. Moreover, most WWTPs are not designed to treat such kind of pollutants, which remain incompletely removed. Currently, biodegradation processes which involved bacterial strains with increased degradation capabilities is one of the most promising technique. The aim of this study was to evaluate the norfloxacin biodegradation potential of the three Bacillus subtilis strains named T-1, T'-1 and I'-1a on a bioreactor scale. The aerobic degradation was conducted in a 5-liter bioreactor on minimal salts medium in co-metabolic culture supplemented with glucose. The degradation rate of norfloxacin was determined with the HPLC technique. The surface tension was determined using ring method in order to observe the changes in biosurfactants production. Also, the biofilm formation abilities of the bacteria with two quantitative methods, crystal violet (CV) method and TTC-based test and enzymes production were evaluated.

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Biofilm mediated decontamination of pollutants from the environment

Cited by 73 publications

References 104 publications

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing Proteinaceous Curli Fimbriae and Cellulose Nanofibers

Application of Genetically Engineered Dioxygenase Producing Pseudomonas putida on Decomposition of Oil from Spiked Soil

Norfloxacin degradation byBacillus subtilisstrains able to produce biosurfactants on a bioreactor scale

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