Biofilm inhibition mechanism from extract of Hymenocallis littoralis leaves

Nadaf, Naiem H.; Parulekar, Rishikesh S.; Patil, Rahul; Gade, Trupti K.; Momin, Anjum A.; Waghmare, Shailesh R.; Dhanavade, Maruti J.; Arvindekar, Akalpita U.; Sonawane, Kailas D.

doi:10.1016/j.jep.2018.04.031

Cited by 28 publications

(14 citation statements)

References 63 publications

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“…Moreover, etomidate was very close to residues of the catalytic triad (Ser170) and to the amino acid residue that has an adhesion role (Lys59), while fluconazole presented greater distances from all these residues. Our data corroborate those reported by [31], who demonstrated the binding to Lys59 of eight compounds extracted from Hymenocallis littorali with activity against C. abicans biofilms. Kioshima et al [11] demonstrated the antibiofilm activity of 24 compounds against C. albicans, and the 5 compounds that showed the best in vitro activity against biofilms also showed the best interactions with Lys59 through molecular docking.…”

Section: Discussionsupporting

confidence: 92%

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Sá

Silva

Neto

et al. 2020

Journal of Medical Microbiology

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This study evaluated the effect of etomidate against biofilms of Candida spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: Candida albicans, Candida parapsilosis and Candida tropicalis. Growing biofilms were exposed to etomidate at 31.25–500 µg ml−1. Then, an ALS3 adhesive protein from C. albicans was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifungal activity against growing biofilms of resistant C. albicans, C. parapsilosis and C. tropicalis at all concentrations used in the study. The etomidate coupling analysis revealed three interactions with the residues of interest compared to hepta-threonine, which remained at the ALS3 site. In addition, etomidate decreased the expression of mannoproteins on the surface of C. albicans. These results revealed that etomidate inhibited the growth of biofilms.

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Section: Discussionsupporting

confidence: 92%

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Sá

Silva

Neto

et al. 2020

Journal of Medical Microbiology

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“…Leaves extract of Hymenocallis littoralis contained multiple bioactive constituents (4-methylesculetin, methylisoeugenol, Quercetin 5,7,3′,4′-tetramethyl ether 3-rutinoside, phenols and flavonoids, etc. ), which showed promising antimicrobial properties against pathogenic microorganisms and biofilm formation [57]. Green tea extract (epigallocatechin-3-gallate, EGCG) from Camellia sinensis could not only suppress Stenotrophomonas maltophilia biofilm formation both in vitro but also inhibit microbial infection in lung of C57BL/6 and Cftr mutant mice [58].…”

Section: Othersmentioning

confidence: 99%

Developing natural products as potential anti-biofilm agents

et al. 2019

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Biofilm is a natural form of bacterial growth ubiquitously in environmental niches. The biofilm formation results in increased resistance to negative environmental influences including resistance to antibiotics and antimicrobial agents. Quorum sensing (QS) is cell-to-cell communication mechanism, which plays an important role in biofilm development and balances the environment when the bacteria density becomes high. Due to the prominent points of biofilms implicated in infectious disease and the spread of multi-drug resistance, it is urgent to discover new antibacterial agents that can regulate biofilm formation and development. Accumulated evidences demonstrated that natural products from plants had antimicrobial and chemo-preventive properties in modulation of biofilm formation in the last two decades. This review will summarize recent studies on the discovery of natural anti-biofilm agents from plants with clear-cut mechanisms or identified molecular addresses, as well as some herbs with unknown mechanisms or unidentified bioactive ingredients. We also focus on the progression of techniques on the extraction and identification of natural anti-biofilm substances. Besides, anti-biofilm therapeutics undergoing clinical trials are discussed. These newly discovered natural anti-biofilm agents are promising candidates which could provide novel strategies for biofilm-associated infections.

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“…The grid box is cantered in such a way that it encloses the entire binding site of both the receptors and provides enough space for translation and rotation of ligands. The generated docked conformation was ranked by predicted binding energy and topmost binding energy docked conformation was analyzed using UCSF Chimera (Pettersen et al, 2004) for intermolecular hydrogen bonding of active site amino acid residues from the receptors with docked ligands (Nadaf et al, 2018).…”

Section: Assessment Of Exopolysaccharide (Eps) Production By Ruthenium Red Stainingmentioning

confidence: 99%

Effect of Adiantum philippense Extract on Biofilm Formation, Adhesion With Its Antibacterial Activities Against Foodborne Pathogens, and Characterization of Bioactive Metabolites: An in vitro-in silico Approach

et al. 2020

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Adiantum philippense (A. philippense), an ethnomedicinally important fern, has become an interesting herb in the search for novel bioactive metabolites, which can also be used as therapeutic agents. Primarily, in this study, A. philippense crude extract was screened for its phytochemical constituents, antagonistic potential, and effect on bacterial adhesion and biofilm formation against common food pathogens. Phytochemical profiling of A. philippense was carried out by using High Resolution-Liquid Chromatography and Mass Spectroscopy (HR-LCMS) followed by antibacterial activity via agar cup/well diffusion, broth microdilution susceptibility methods, and growth curve analysis. Antibiofilm potency and efficacy were assessed on the development, formation, and texture of biofilms through light microscopy, fluorescent microscopy, scanning electron microscopy, and the assessment of exopolysaccharide production. Correspondingly, a checkerboard test was performed to evaluate the combinatorial effect of A. philippense and chloramphenicol. Lastly, molecular docking studies of identified phytochemicals with adhesin proteins of tested food pathogens, which helps the bacteria in surface attachment and leads to biofilm formation, were assessed. A. philippense crude extract was found to be active against all tested food pathogens, displaying the rapid time-dependent kinetics of bacterial killing. A. philippense crude extract also impedes the biofilm matrix by reducing the total content of exopolysaccharide, and, likewise, the microscopic images revealed a great extent of disruption in the architecture of biofilms. A synergy was observed between A. philippense crude extract and chloramphenicol for E. coli, S. aureus, and P. aeruginosa, whereas an additive effect was observed for S. flexneri. Various

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Biofilm inhibition mechanism from extract of Hymenocallis littoralis leaves

Cited by 28 publications

References 63 publications

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Developing natural products as potential anti-biofilm agents

Effect of Adiantum philippense Extract on Biofilm Formation, Adhesion With Its Antibacterial Activities Against Foodborne Pathogens, and Characterization of Bioactive Metabolites: An in vitro-in silico Approach

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