Biofilm inactivation by synergistic treatment of atmospheric pressure plasma and chelating agents

Tschang, Chen-Yon Tobias; Thoma, Markus H.

doi:10.1016/j.cpme.2019.100091

Cited by 8 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of nonthermal plasma (NTP) have shown that it can be utilised for a wide range of applications, including food preservation, wound sterilisation, enhanced crop growth, pollution abatement, volatile organic compound removal, polymer functionalisation and water purification. [ 1–5 ] Such a broad variability of applications is due to the large range of gas chemistries that can be generated using NTP systems. By using ambient air, reactive oxygen species (ROS) and reactive nitrogen species (RNS) can be generated to interact with target samples.…”

Section: Introductionmentioning

confidence: 99%

Diagnostics of a large volume pin‐to‐plate atmospheric plasma source for the study of plasma species interactions with cancer cell cultures

Scally

Behan

Carvalho

et al. 2021

Plasma Processes & Polymers

View full text Add to dashboard Cite

A large gap pin‐to‐plate, atmospheric‐pressure plasma reactor is demonstrated as means of in vitro study of plasma species interactions with cell cultures. By employing optical emission and optical absorption spectroscopy, we report that the pin‐to‐pate plasma array had an optimal discharge frequency for cell death of 1000 Hz in ambient air for the target cancer cell line, human glioblastoma multiform (U‐251MG). The detected plasma chemistry contained reactive oxygen and nitrogen species including OH, N2, N2+ and O3. We show that by varying the plasma discharge frequency, the plasma chemistry can be tailored to contain up to 8.85 times higher levels of reactive oxygen species (ROS) as well as a factor increase of up to 2.86 for levels of reactive nitrogen species (RNS). At higher frequencies, ROS are more dominant than RNS, which allows for a more dynamic and controlled environment for sample study without modifying the inducer gas conditions. When used for treatment of culture media and cell cultures, variation of the plasma discharge frequency over the range 1000–2500 Hz demonstrated a clear dependence of the responses, with the highest cytotoxic responses observed for 1000 Hz. We propose that the reactor offers a means of studying plasma–cell interactions and possible cofactors such as pro‐drugs and nanoparticles for a large volume of samples and conditions due to the use of well plates.

show abstract

Section: Introductionmentioning

confidence: 99%

Diagnostics of a large volume pin‐to‐plate atmospheric plasma source for the study of plasma species interactions with cancer cell cultures

Scally

Behan

Carvalho

et al. 2021

Plasma Processes & Polymers

View full text Add to dashboard Cite

show abstract

“…The SMD we applied is a DBD-based plasma source which consists of an aluminum plate (power electrode), quartz plate (dielectric), and stainless steel mesh (grounded electrode) (figure 1) [17,20,40]. The configuration of the power electrode, dielectric, and ground electrode is 125 × 85 × 1 mm, 150 × 110 × 1 mm, and 150 × 110 × 1.2 mm, respectively.…”

Section: Surface Micro-discharge (Smd)mentioning

confidence: 99%

In vitrocomparison of direct plasma treatment and plasma activated water onEscherichia coliusing a surface micro-discharge

Tschang

Thoma

2019

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

In this study, our aim is to compare the inactivation of bacteria by direct plasma exposure of bacterial solution and plasma activated water (PAW) using a surface micro-discharge (SMD). Four potential factors which could affect both treatments were tested, namely the effect of low dose reactive oxygen species (ROS) pretreatment, enzymatic defence mechanism against oxidative stress, extracellular molecules, and mass transfer in liquid. We applied two power modes of the SMD, i.e. low power ozone mode and high power mode, to inactivate () in distilled water either by direct treatment (DT) or PAW. ROS pretreatment was tested by 30 M of , while superoxide dismutase (SOD) and catalase were used to investigate the effect of enzymatic antioxidant defense. Furthermore, the effect of extracellular molecules was examined by centrifuging and mass transfer in liquid by vortexing. In addition, reactive species in the solution were measured by titanium sulfonate, and nitrite/nitrate colorimetric method for , and and , respectively. Results indicate that vortexing and centrifuging significantly influence the bacterial reduction, while pretreatment could not induce resistance of bacteria against plasma treatment. Enzymatic antioxidants of SOD and catalase could potentially effect the inactivation and plasma chemistry yet only if the concentrations are sufficiently high. We assume that the continuous generation of peroxynitrite is crucial in plasma inactivation for both DT and PAW under high power condition, while under low power condition, long-term bactericidal affects are limited due to the decomposition of ozone by nitrite.

show abstract

“…Since the last two decades, high potential has been shown from studies on atmospheric pressure plasma jets (APPJ) and their application in the biomedical field, for instance microbial inactivation, cancer treatment, and wound healing [1][2][3][4][5][6][7][8]. The advantage of applying APPJ are the relative high efficiency, flexibility, and low cost compared to conventional treatment.…”

Section: Introductionmentioning

confidence: 99%

Effect of external axial magnetic field on a helium atmospheric pressure plasma jet and plasma-treated water

Tschang

Bergert

Mitic

et al. 2020

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

Potential in biomedical-related applications by atmospheric pressure plasma-treated water gradually increased recently. In order to enhance the generation of reactive species in atmospheric pressure plasma in regards to liquid treatment, this study aims to investigate the effect of external axial magnetic field on a helium atmospheric pressure plasma jet (APPJ) and plasma-irradiated water. Magnetic field strength up to 2.0 T was generated by an electromagnet in Helmholtz configuration. The dielectric barrier discharge-based APPJ is driven by 4.4 slm helium and sinusoidal 30 kHz, 10 k V p p signals. Plasma was mainly characterized by optical emission where vibrational and rotational temperature was estimated by nitrogen second positive system, and electron temperature and density by emission intensity line ratio method. Representative reactive species of nitrite, nitrate, and hydrogen peroxide concentration in the plasma-treated water were also quantified. Results of vibrational temperature and electron density showed a first drop then rise trend which partially corresponds to the reactive species in the plasma-treated water. No significant changes were found in rotational temperature, while electron temperature monotonically increased with the magnetic field.

show abstract

Biofilm inactivation by synergistic treatment of atmospheric pressure plasma and chelating agents

Cited by 8 publications

References 38 publications

Diagnostics of a large volume pin‐to‐plate atmospheric plasma source for the study of plasma species interactions with cancer cell cultures

Diagnostics of a large volume pin‐to‐plate atmospheric plasma source for the study of plasma species interactions with cancer cell cultures

In vitrocomparison of direct plasma treatment and plasma activated water onEscherichia coliusing a surface micro-discharge

Effect of external axial magnetic field on a helium atmospheric pressure plasma jet and plasma-treated water

Contact Info

Product

Resources

About