Biofilm-Forming Ability and Clonality in Acinetobacter baumannii Strains Isolated from Urine Samples and Urinary Catheters in Different European Hospitals

Vuotto, Claudia; Grosso, Filipa; Longo, Francesca; Balice, Maria Pia; Barros, Mariana Carvalho de; Peixe, Luı́sa; Donelli, Gianfranco

doi:10.1007/5584_2017_70

Cited by 5 publications

(4 citation statements)

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“…In this way, A. baumannii might persist in clinical devices and hospital environments, thus causing HAIs and outbreaks worldwide [ 95 , 114 ]. Supporting this, several studies have reported biofilm formation ability in A. baumannii strains causing various types of infection: surgical site and wound infections [ 115 , 116 ], urinary tract infections [ 117 , 118 ], blood and respiratory infections, and skin and soft infections [ 119 , 120 , 121 ].…”

Section: Clinical Impact Of a Baumannii Biofilmsmentioning

confidence: 79%

Gram-Negative Bacteria Holding Together in a Biofilm: The Acinetobacter baumannii Way

et al. 2021

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Bacterial biofilms are a serious public-health problem worldwide. In recent years, the rates of antibiotic-resistant Gram-negative bacteria associated with biofilm-forming activity have increased worrisomely, particularly among healthcare-associated pathogens. Acinetobacter baumannii is a critically opportunistic pathogen, due to the high rates of antibiotic resistant strains causing healthcare-acquired infections (HAIs). The clinical isolates of A. baumannii can form biofilms on both biotic and abiotic surfaces; hospital settings and medical devices are the ideal environments for A. baumannii biofilms, thereby representing the main source of patient infections. However, the paucity of therapeutic options poses major concerns for human health infections caused by A. baumannii strains. The increasing number of multidrug-resistant A. baumannii biofilm-forming isolates in association with the limited number of biofilm-eradicating treatments intensify the need for effective antibiofilm approaches. This review discusses the mechanisms used by this opportunistic pathogen to form biofilms, describes their clinical impact, and summarizes the current and emerging treatment options available, both to prevent their formation and to disrupt preformed A. baumannii biofilms.

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Section: Clinical Impact Of a Baumannii Biofilmsmentioning

confidence: 79%

Gram-Negative Bacteria Holding Together in a Biofilm: The Acinetobacter baumannii Way

et al. 2021

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“…Except for sputum samples, AB strains isolated from urine or urinary catheters samples have been previously reported [ 35 , 36 ]. Only 8 of 109 CR-AB strains in our study were proved to be strong or moderate biofilm producers, suggesting that it may be related to the nature of the A. baumannii clinical isolates [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of carbapenem-resistant hypervirulent Acinetobacter baumannii strains isolated from hospitalized patients in the mid-south region of China

Luo

et al. 2020

BMC Microbiol

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Background Acinetobacter baumannii has traditionally been considered an opportunistic pathogen with low virulence. In this study, we characterized the carbapenem-resistant hypervirulent A. baumannii (CR-hvAB) stains isolated from our hospital in mid-south region of China. Results Blood samples collected between January 2017 and May 2019 were used for virulence experiments and biofilm assays of individual carbapenem-resistant A. baumannii (CR-AB) strains, performed using a Galleria mellonella infection model and crystal violet staining method, respectively. CR-AB isolates that induced high mortality in the G. mellonella infection model were subjected to genotyping, susceptibility testing, and clinical data analysis, and the genetic characterization of these isolates was performed by whole-genome sequencing (WGS). Among the 109 CR-AB clinical strains, the survival rate of G. mellonella larvae infected with 7 (6.4%) CR-AB isolates (number of strains with mortality of 0, 10 and 20% was 4, 1, and 2, respectively), was significantly lower than that of A. baumannii ATCC 19606 (100.0%) and the remaining CR-AB isolates (> 80.0%). Consistent with these results, patients infected with these seven isolates had an average 7-day mortality rate of 42.9%, suggesting that the isolates were CR-hvAB. These seven isolates belonged to four sequence types (STs): ST457, ST195, ST369, and ST2088 (a new ST), and mainly ST457 (n = 4). The results of the biofilm study showed that eight strains had powerful biofilm ability (strong [n = 1] and moderate [n = 7] biofilm producers) including these seven CR-hvAB isolates. Conclusions CR-hvAB isolates that induced a high mortality rate were cloned in our hospital, most of which belonged to ST457; thus, monitoring of these strains, particularly ST457, should be strengthened in the future. Meanwhile, A. baumannii, which was isolated from blood specimens and found to powerful biofilm-forming ability, is a probable hvAB isolate.

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“…Ec5FSL and Ec9FSL isolates were grown overnight at 37 • C in Luria-Bertani (LB) broth. Quantitative biofilm production assay was performed as described in Vuotto et al [11]. Each plate was incubated for 20 h at 37 • C, and experiments were repeated three times.…”

Section: Quantitative Biofilm Production Assaymentioning

confidence: 99%

“…[8]. It is increasingly common, especially in hospitalized patients, to isolate from their urines multidrug-and extensively drug-resistant microorganisms, such as extended-spectrum beta-lactamase (ESBL)-producing bacteria, AmpC β-lactamases producers, and carbapenem-resistant Enterobacteriaceae [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

et al. 2020

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Poloxamers are nontoxic, amphiphilic copolymers used in different formulations. Due to its surfactant properties, Poloxamer 338 (P388) is herein proposed as a strategy to avoid biofilm formation often causing recalcitrant catheter-associated urinary tract infections (CAUTI). The aim is to evaluate the ability of P388 coatings to affect the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. Attenuated total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle measurement were employed to characterize the P388-coated silicone catheter in terms of amount of P388 layered, coating thickness, homogeneity, and hydrophilicity. In static conditions, the antifouling power of P388 was defined by comparing the E. coli cells adherent on a hydrophilic P388-adsorbed catheter segment with those on an uncoated one. A P388-coated catheter, having a homogeneous coverage of 35 nm in thickness, reduced of 0.83 log10 and 0.51 log10 the biofilm of Ec5FSL and Ec9FSL, respectively. In dynamic conditions, the percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic system, simulating the in vivo conditions of catheterized patients. As a result, both E. coli isolates were undetected. The strong and stable antifouling property against E. coli biofilm lead us to consider P388 as a promising anti-biofilm agent for CAUTIs control.

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Biofilm-Forming Ability and Clonality in Acinetobacter baumannii Strains Isolated from Urine Samples and Urinary Catheters in Different European Hospitals

Abstract: An evolution towards a decrease in adhesion ability and a CHDL content change was observed along the years in several European countries.

Cited by 5 publications

References 35 publications

Gram-Negative Bacteria Holding Together in a Biofilm: The Acinetobacter baumannii Way

Gram-Negative Bacteria Holding Together in a Biofilm: The Acinetobacter baumannii Way

Characterization of carbapenem-resistant hypervirulent Acinetobacter baumannii strains isolated from hospitalized patients in the mid-south region of China

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

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