Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

Kwiecińska-Piróg, Joanna; Przekwas, Jana; Majkut, Michał; Skowron, Krzysztof; Gospodarek‐Komkowska, Eugenia

doi:10.3390/microorganisms8111823

Cited by 16 publications

(15 citation statements)

References 59 publications

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“…A wound is considered chronic if its healing is delayed after two to eight weeks. Chronic wound infections are challenging due to the biofilm formation that provide bacterial resistance to antibiotics by extracellular barrier of polymeric substances [ 25 , 31 ]. Honey is effective in reducing the biofilm of both drug-sensible and drug-resistant strains of Gram-positive and Gram-negative bacteria [ 18 , 22 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Honey dressing: a missed way for orthopaedic wound care

Ahmed

Eltregy

Kandil

2022

International Orthopaedics (SICOT)

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Purpose Orthopaedic-related wounds are critical situations calling for care to avoid deep infections and its consequences. The purpose of this study was to evaluate the efficacy of using honey for care of orthopaedic-related wounds with limited resources. Patients and methods This prospective study included 50 cases with an average age of 38.18 (range 17–63) years with 38 males and 12 females. The most frequent wound location was the leg (41 patients; 82%), then the foot (six patients; 12%), and the ankle in three patients (6%). The aetiologies were open fractures (34 cases; 68%), infected tibial non-unions (nine cases; 18%), and post-operative infections (seven cases; 14%). Exposed tendon was present in three cases. Bone exposure was present in two cases. Deep infection was present in 29 cases (58%). Besides treating the primary cause, a ribbon of gauze soaked with honey was applied to the wounds after thorough saline washing. Results Wound sizes were variable. All cases showed improvement in all parameters with complete wound healing and full coverage of bone and tendons. Recurrence of deep infection occurred in three cases and treated by debridement. One case needed sequestrectomy of a small exposed tibial cortical fragment. Exposed tendon cases showed superficial necrosis which was treated by simple debridement. Initial mild itching occurred in five patients with spontaneous resolution. Conclusion With treating the underlying aetiology and optimising the patient’s general condition, honey was an effective, simple, and affordable method of wound care in different orthopaedic conditions even with exposed bone or tendons.

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Section: Discussionmentioning

confidence: 99%

Honey dressing: a missed way for orthopaedic wound care

Ahmed

Eltregy

Kandil

2022

International Orthopaedics (SICOT)

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“…Due to the constant development of resistance to drugs by bacteria, propolis can be a feasible treatment option because its biological activity is a consequence of the synergism of various chemical compounds present in the extract, and the combination with antimicrobial agents may allow lower doses of antibiotics (7). Propolis can reduce biofilm formation and accelerate healing processes (8), especially in cases of chronic infections that are difficult to control due to biofilm formation in the wound environment (9). This situation was evident in clinical isolates of Proteus mirabilis from patients with chronic wounds, for which ethanolic extracts of propolis were effective in inhibiting the formation of biofilm (10).…”

Section: Introductionmentioning

confidence: 99%

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.

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“…On the other hand, several studies explained the healing power of MH via its various antioxidant, antimicrobial, and anti-inflammatory effects on the inflammatory response [56,57].…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits

Mousa

Allemailem

Alhumaydhi

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Patients treated with cyclophosphamide (CP) usually suffer from severe hemorrhagic cystitis (HC). Our previous study exhibited that mesna + celery cotherapy partially ameliorated HC. Therefore, there is a substantial need to seek alternative regimens to get complete protection against CP-induced HC. The current study investigated the effects of mesna + celery seed oil (MCSO) or mesna + manuka honey (MMH) cotherapy against CP-induced HC in adult male rabbits. The forty rabbits were divided into four equal groups and treated for three weeks. The control group (G1) received distilled water and the second group (G2) received CP (50 mg/kg/week). The third group (G3) received CP + MCSO (CPMCSO regimen), and the fourth group (G4) received CP + MMH (CPMMH regimen). The urinary bladder (UB) specimens were processed to evaluate UB changes through histopathological, immunohistochemical, ultrastructural, and biochemical investigations. In G2, CP provoked HC features (urothelial necrosis, ulceration, and sloughing), UB fibrosis, and TNF-α immunoexpression. Besides, CP reduced the activity of antioxidant enzymes (GPx1, SOD3, and CAT) and elevated the serum levels of NF-κB, TNF-α, IL-1B, and IL-6 cytokines in G2 rabbits. In contrast, the CPMMH regimen caused significant increments of UB protection against HC in G4 rabbits compared to the partial protection by the CPMCSO regimen in G3. Therefore, our study indicated for the first time that the novel CPMMH regimen resulted in complete UB protection against CP-induced HC via combined antioxidant, anti-inflammatory, and antifibrotic properties.

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Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

Cited by 16 publications

References 59 publications

Honey dressing: a missed way for orthopaedic wound care

Honey dressing: a missed way for orthopaedic wound care

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits

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