Biofilm formation on the surface of polylactide during its biodegradation in different environments

Walczak, Maciej; Brzezinska, Maria Swiontek; Sionkowska, Alina; Michalska, Marta; Jankiewicz, Urszula; Deja-Sikora, Edyta

doi:10.1016/j.colsurfb.2015.09.036

Cited by 43 publications

(16 citation statements)

References 34 publications

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“…In our previous study, we also observed biofilm formation by our isolate P. aeruginosa strain S3 on the surface of PLA during the process of biodegradation [12]. Phenomenon of biofilm formation on the surface of PLA had previously been reported by other authors as well [65–67].…”

Section: Resultssupporting

confidence: 72%

Genome annotation of Poly(lactic acid) degradingPseudomonas aeruginosaandSphingobacterium sp.

Shah

Auras

et al. 2019

Preprint

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25Pseudomonas aeruginosa and Sphinogobacterium sp. are well known for their ability to 26 decontaminate many environmental pollutants like PAHs, dyes, pesticides and plastics. The 27 present study reports the annotation of genomes from P. aeruginosa and Sphinogobacterium sp. 28 that were isolated from compost, based on their ability to degrade poly(lactic acid), PLA, at 29 mesophillic temperatures (~30ºC). Draft genomes of both the strains were assembled from 30 Illumina reads, annotated and viewed with an aim of gaining insight into the genetic elements 31 involved in degradation of PLA. The draft-assembled genome of strain Sphinogobacterium strain 32 S2 was 5,604,691 bp in length with 435 contigs (maximum length of 434,971 bp) and an average 33 G+C content of 43.5%. The assembled genome of P. aeruginosa strain S3 was 6,631,638 bp long 34 with 303 contigs (maximum contig length of 659,181 bp) and an average G+C content 66.17 %. 35A total of 5,385 (60% with annotation) and 6,437 (80% with annotation) protein-coding genes 36 were predicted for strains S2 and S3 respectively. Catabolic genes for biodegradation of xenobiotic 37 and aromatic compounds were identified on both draft genomes. Both strains were found to have 38 the genes attributable to the establishment and regulation of biofilm, with more extensive 39 annotation for this in S3. The genome of P. aeruginosa S3 had the complete cascade of genes 40 involved in the transport and utilization of lactate while Sphinogobacterium strain S2 lacked 41 lactate permease, consistent with its inability to grow on lactate. As a whole, our results reveal and 42 predict the genetic elements providing both strains with the ability to degrade PLA at mesophilic 43 temperature. 44 45 46 47 1. Introduction 48 Poly(lactic acid) (PLA), is a bio-based aliphatic polyester polymer, obtained from sources such as 49 corn sugar, cassava, wheat, rice, potato, and sugar cane, considered renewable [1, 2]. PLA is 50 completely biodegradable under industrial composting conditions [3] as well as under 51 unsupervised environmental conditions where its biodegradation is considered safe [4]. In the last 52 two decades biodegradation of PLA has been extensively studied and many microbial species 53 (actinomycete, bacteria, fungus) have been identified with the ability to degrade PLA [4]. Most of 54 the reported bacterial species are from the family Pseudonocardiaceae, Thermomonosporaceae, 55 Micromonosporaceae, Streptosporangiaceae, Bacillaceae and Thermoactinomycetaceae while 56 the fungal species are mainly from the phylum Basidiomycota (Tremellaceae) and Ascomycota 57 (Trichocomaceae, Hypocreaceae) [5-11]. 58In our previous study we also described four bacterial strains designated as S1, S2, S3 and S4, able 59 to degrade PLA at ambient temperature [12]. Two of the isolated strains, Sphingobacterium sp. 60(S2) and P. aeruginosa (S3), were also evaluated for their PLA degradation in soil microcosms 61 [13]. The genus Sphingobacterium is from Phylum Bacteriodetes, Family Sphingobacteria...

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Section: Resultssupporting

confidence: 72%

Genome annotation of Poly(lactic acid) degradingPseudomonas aeruginosaandSphingobacterium sp.

Shah

Auras

et al. 2019

Preprint

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“…The degradation differs from one microbe to another because different microbes exhibit different characteristics (Balasubramanian et al, 2010). However, Walczak et al (2015) reported that “the abundance of biofilm formed on polyethylene surface by specific bacterial strains does not always correlate with their hydrolytic activity, which is important in the context of biodegradation.” Our results support the theory as indicated by bacteria strains ISJ36 and ISJ38 displaying moderate hydrophobicity and protein content but exhibited considerably less biodegradation (i.e., 0.7% and 0.8%, respectively). The differences among isolates with respect to PE degradation may be due to the variations that exist in metabolic rate as well as the polymer uptake mechanism.…”

Section: Resultsmentioning

confidence: 99%

Biofilm mediated degradation of commercially available LDPE films by bacterial strains isolated from partially degraded plastic

Gupta

Devi

2020

Remediation Journal

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Biodegradation is an attractive approach for the elimination of synthetic polymers, pervasively accumulated in natural environments and generating ecological problems. The present work investigated the degradation of low‐density polyethylene (PE) by three Bacillus sp., that is, ISJ36, ISJ38, and ISJ40. The degree of biodegradation was assessed by measuring hydrophobicity, viability, and total protein content of bacterial biofilm attached to the PE surface. Although all three bacterial strains were able to establish an active biofilm community on the PE surface, ISJ40 showed better affinity toward PE degradation than the other two. Bacterial colonization and physical changes on the PE surface were visualized by scanning electron microscopy. Fourier transform infrared spectroscopy analysis revealed alteration in the intensities of functional groups along with an increase in the carbonyl bond indexes. The study results suggest that the Bacillus strain ISJ40 can be used as a potential degrader for the eco‐friendly treatment of PE waste.

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“…The potential of polymeric materials as good bacterial carriers for industrial applications has been described before. This is the case of PLA surfaces, which have been previously tested as biofilm carriers in bioremediation studies [51][52][53] . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 confirms that the biofilm formation of the selected strains in late colonization stages is not easily affected by the electrospun microfibers diameter.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

Tamayo-Ramos

Rumbo

Caso³

et al. 2018

ACS Appl. Mater. Interfaces

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Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA.

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Biofilm formation on the surface of polylactide during its biodegradation in different environments

Cited by 43 publications

References 34 publications

Genome annotation of Poly(lactic acid) degradingPseudomonas aeruginosaandSphingobacterium sp.

Genome annotation of Poly(lactic acid) degradingPseudomonas aeruginosaandSphingobacterium sp.

Biofilm mediated degradation of commercially available LDPE films by bacterial strains isolated from partially degraded plastic

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

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