Biofilm formation of two genetically diverse Staphylococcus aureus isolates under beta-lactam antibiotics

Liang, Jie; Huang, Teng Yi; Mao, Yuzhu; Li, Xuejie

doi:10.3389/fmicb.2023.1139753

Cited by 5 publications

(2 citation statements)

References 96 publications

(107 reference statements)

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“…When administered orally, ampicillin concentration in saliva can reach peak concentrations close to or higher than minimum inhibitory concentrations (MIC) for several of the most studied bacteria in the oral cavity 31 , 32 . At concentrations below the MIC, ampicillin and several other antibiotics have shown to stimulate biofilm formation by S. aureus and H. influenzae , as well as by other pathogens found in the oral cavity and at close anatomical sites, such as Streptococcus intermedius and Enterococcus faecalis 13 , 33 – 36 .…”

Section: Introductionmentioning

confidence: 99%

Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome

Brar,

Dhariwal,

Åmdal

et al. 2024

npj Biofilms Microbiomes

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Prolonged exposure to antibiotics at low concentration can promote processes associated with bacterial biofilm formation, virulence and antibiotic resistance. This can be of high relevance in microbial communities like the oral microbiome, where commensals and pathogens share a common habitat and where the total abundance of antibiotic resistance genes surpasses the abundance in the gut. Here, we used an ex vivo model of human oral biofilms to investigate the impact of ampicillin on biofilm viability. The ecological impact on the microbiome and resistome was investigated using shotgun metagenomics. The results showed that low concentrations promoted significant shifts in microbial taxonomic profile and could enhance biofilm viability by up to 1 to 2-log. For the resistome, low concentrations had no significant impact on antibiotic resistance gene (ARG) diversity, while ARG abundance decreased by up to 84%. A positive correlation was observed between reduced microbial diversity and reduced ARG abundance. The WHO priority pathogens Streptococcus pneumoniae and Staphylococcus aureus were identified in some of the samples, but their abundance was not significantly altered by ampicillin. Most of the antibiotic resistance genes that increased in abundance in the ampicillin group were associated with streptococci, including Streptococcus mitis, a well-known potential donor of ARGs to S. pneumoniae. Overall, the results highlight the potential of using the model to further our understanding of ecological and evolutionary forces driving antimicrobial resistance in oral microbiomes.

show abstract

Section: Introductionmentioning

confidence: 99%

Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome

Brar,

Dhariwal,

Åmdal

et al. 2024

npj Biofilms Microbiomes

View full text Add to dashboard Cite

show abstract

“…When administered orally, ampicillin concentration in saliva can reach peak concentrations close to or higher than minimum inhibitory concentrations (MIC) for several of the most studied bacteria in the oral cavity (30,31). At concentrations below the MIC, ampicillin and several other antibiotics have shown to stimulate bio lm formation by S. aureus and H. in uenzae, as well as by other pathogens found in the oral cavity and at close anatomical sites, such as Streptococcus intermedius and Enterococcus faecalis (12,(32)(33)(34)(35).…”

Section: Introductionmentioning

confidence: 99%

Ecological consequences of low ampicillin concentrations on an ex-vivo biofilm model of the human oral microbiome

Petersen,

Brar,

Dhariwal

et al. 2023

Preprint

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Antibiotics at low concentration can promote processes such as biofilm formation, virulence and antibiotic resistance. This can be of high relevance in microbial communities like the oral microbiome, where commensals and pathogens share a common habitat and where the abundance of antibiotic resistance genes surpasses the abundance in the gut. Here, we used an ex vivo model of human oral biofilms to investigate the impact of ampicillin on biofilm viability. Further, the ecological impact on the microbiome and resistomes was investigated using shotgun metagenomics. The results showed that low concentrations promoted significant shifts in microbial taxonomic profile and could enhance biofilm viability by up to 1 to 2-log. For the resistome, low concentrations had no significant impact on antibiotic resistance gene (ARG) diversity, while ARG abundance decreased by up to 84%. A positive correlation was observed between reduced microbial diversity and reduced ARG abundance. The WHO priority pathogens Streptococcus pneumoniae and Staphylococcus aureus were identified in some of the samples, but their abundance was not significantly altered by ampicillin. Yet, most of the antibiotic resistance genes that increased in abundance in the ampicillin group were associated with streptococci, including Streptococcus mitis, a well-known potential donor of ARGs to S. pneumoniae. To our knowledge, this is the first report on antibiotic effects on oral microbial communities using an ex-vivo human microbiome model combining biofilms and shotgun metagenomics. Overall, the results highlight the potential of using the model to further our understanding of ecological and evolutionary forces driving antimicrobial resistance in oral microbiomes.

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Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children

Xiao,

Su,

Chen

et al. 2024

Microbial Pathogenesis

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Biofilm formation of two genetically diverse Staphylococcus aureus isolates under beta-lactam antibiotics

Cited by 5 publications

References 96 publications

Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome

Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome

Ecological consequences of low ampicillin concentrations on an ex-vivo biofilm model of the human oral microbiome

Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children

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