Biofilm formation of Listeria monocytogenes and its resistance to quaternary ammonium compounds in a simulated salmon processing environment

Pang, Xinyi; Wong, Chun Hong; Chung, Hyun‐Jung; Yuk, Hyun‐Gyun

doi:10.1016/j.foodcont.2018.11.029

Cited by 58 publications

(39 citation statements)

References 40 publications

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“…An intra-specific variability in biofilm architecture has also been reported by other authors. Among the different structures seen are one-cell-thick layers, flat layers of cells consisting of several layers without a given structure, honeycomb structures, and networks of interwoven clumps [35][36][37][38][39][40][41][42]. The great variability observed in the structure of the biofilms formed by Listeria spp.…”

Section: Discussionmentioning

confidence: 99%

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

2019

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Biofilms are a key factor in the persistence of Listeria in food processing plants, representing a potential source of foodstuff contamination. Nine Listeria strains (eight Listeria monocytogenes and one Listeria ivanovii) were studied by confocal laser scanning microscopy (CLSM) for their ability to form biofilm on glass, polystyrene, graphene and resin after 120 h of incubation at 12 °C. The relationship between cell surface hydrophobicity and biofilm formation was also investigated. On comparing the data for all the strains, similar (P > 0.05) biovolume values were obtained on glass (average 3.39 ± 1.69 µm3/µm2) and graphene (2.93 ± 1.14 µm3/µm2), while higher (P < 0.05) values were observed for polystyrene (4.39 ± 4.14 µm3/µm2). The highest (P < 0.01) biovolume levels were found in the biofilms formed on resin (7.35 ± 1.45 µm3/µm2), which also had the smallest biomass of inactivated cells (0.38 ± 0.37 µm3/µm2 vs. 1.20 ± 1.12 µm3/µm2 on the remaining surfaces; P < 0.001). No relationship was noted between cell surface hydrophobicity and biofilm-forming ability.

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Section: Discussionmentioning

confidence: 99%

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

2019

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“…This study investigated the possibilities of applying UV and/or chemical sanitizers in order to enhance the microbial safety of hydroponic farms. Doses of UV (250 mJ/cm 2 ) and QAC (200 ppm, 15 min) used in this study were recognized as harsh treatments by previous literature (Li et al, 2014; Pang et al, 2019). This study showed that 250 mJ/cm 2 UV treatment induced higher reductions (5 to 6-log reduction) than a QAC treatment of 200 ppm for 15 min (1 to 2-log reduction) for all of the tested S. maltophilia strains in biofilms, indicating that it is a more effective and feasible strategy for adoption by hydroponic farms to treat their nutrient solutions that come into contact with the plants and any relevant surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…In the natural environment, biofilms are usually formed by multiple bacterial species, and mixed-species biofilms are reported to show increased resistance to multiple treatments in comparison with single-species biofilms (Modic et al, 2017; Pang et al, 2019). Therefore, in this study, in order to investigate whether the inactivating effect of UV could be attenuated by the presence of other bacteria species, the UV-resistance of S. maltophilia was not only tested in single-species biofilms but also in dual-species biofilms.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Inactivation of Stenotrophomonas maltophilia From Leafy Green Vegetables and Urban Agriculture Systems

Wong

Seet

et al. 2019

Front. Microbiol.

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Stenotrophomonas maltophilia is an emerging opportunistic pathogen that, on the one hand, causes severe nosocomial infection in immunocompromised populations with a high mortality rate and, on the other hand, is present ubiquitously in the environment. This study, for the first time to the best of our knowledge, isolated and characterized S. maltophilia from leafy green vegetables produced by hydroponic farms and from a hydroponic farming facility in Singapore. Eleven S. maltophilia isolates were obtained from three types of leafy green vegetables (sweet basil, kale, and parsley) and from the nutrient solution used by a hydroponic farm. The antimicrobial resistance (AMR), biofilm-forming ability, and resistance to UV and quaternary ammonium compound (QAC) treatments were investigated, as was the fate of S. maltophilia in a simulated leafy green vegetable environment during a storage period of 6 days at different temperatures. The results showed that high population levels of S. maltophilia could be reached on leafy green vegetables, especially after being stored at abused temperatures (>8-log CFU/ml in basil juice after 6 days storage at 20°C) and on hydroponic farming facilities, probably due to biofilm formation (8 to 9-log CFU/well in biofilms). At 4°C, S. maltophilia was able to survive, but no growth was observed during storage in either bacteria culture media or basil juice for a period of 6 days. UV treatment, which induced substantial reductions in S. maltophilia in both single-species and dual-species biofilms mixed with Salmonella enterica serovar Typhimurium reference strain (ATCC 14028) or self-isolated Pseudomonas fluorescens (>4-log reductions by 250 mJ/cm2 UV), is recommended for employment by hydroponic farms to treat their nutrient solutions and farming facilities so as to enhance microbial safety.

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“…One common interaction among microorganisms in mixed-species biofilms is the competition for nutrient acquisition and space occupation. Pang et al (2019) showed that indigenous microbial cells in the runoff fluids of fresh salmon compete with Listeria monocytogenes for nutrients in mixed-species biofilms, resulting in a remarkable reduction in the number of L. monocytogenes cells compared with that in monospecies biofilms. Toxic substances, such as bacteriocins, hydrogen peroxide, organic acids, and enzymes, which are secreted by some microbial species provide competitive advantages over other species within mixed-species biofilms (Rendueles and Ghigo, 2012).…”

Section: Microbial Interactions In Mixed-species Biofilmsmentioning

confidence: 99%

Fighting Mixed-Species Microbial Biofilms With Cold Atmospheric Plasma

et al. 2020

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Most biofilms in nature are formed by multiple microbial species, and such mixedspecies biofilms represent the actual lifestyles of microbes, including bacteria, fungi, viruses (phages), and/or protozoa. Microorganisms cooperate and compete in mixedspecies biofilms. Mixed-species biofilm formation and environmental resistance are major threats to water supply, food industry, and human health. The methods commonly used for microbial eradication, such as antibiotic or disinfectant treatments, are often ineffective for mixed-species biofilm consortia due to their physical matrix barrier and physiological interactions. For the last decade, an increasing number of investigations have been devoted to the usage of cold atmospheric plasma (CAP), which is produced by dielectric barrier discharges or plasma jets to prevent or eliminate microbial biofilms. Here, we summarized the production of CAP, the inactivation of microorganisms upon CAP treatment, and the microbial factors affecting the efficacy of CAP procedure. The applications of CAP as antibiotic alternative strategies for fighting mixed-species biofilms were also addressed.

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Biofilm formation of Listeria monocytogenes and its resistance to quaternary ammonium compounds in a simulated salmon processing environment

Cited by 58 publications

References 40 publications

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

Isolation, Characterization, and Inactivation of Stenotrophomonas maltophilia From Leafy Green Vegetables and Urban Agriculture Systems

Fighting Mixed-Species Microbial Biofilms With Cold Atmospheric Plasma

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